QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (46) Citing Articles via Google Scholar Google Scholar Articles by Shitaka, Y. Articles by Katsuki, H. Search for Related Content PubMed PubMed Citation Articles by Shitaka, Y. Articles by Katsuki, H.

 Previous Article  |  Next Article 

Volume 16, Number 20, Issue of October 15, 1996 pp. 6476-6489
Copyright ©1996 Society for Neuroscience

Basic Fibroblast Growth Factor Increases Functional L-Type Ca²⁺ Channels in Fetal Rat Hippocampal Neurons: Implications for Neurite Morphogenesis In Vitro

Received April 22, 1996; revised July 25, 1996; accepted July 30, 1996.

Yoshitsugu Shitaka, Norio Matsuki, Hiroshi Saito, and Hiroshi Katsuki

Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

Basic fibroblast growth factor (bFGF) is a potent neurotrophic factor that regulates cell proliferation and differentiation during neuronal development. Here we report that fetal hippocampal neurons chronically treated with bFGF displayed larger [Ca²⁺]_i increases than nontreated neurons in response to high K⁺-induced depolarization. This [Ca²⁺]_i response was abolished by nicardipine and was little affected by treatments that depleted intracellular Ca²⁺ stores, thus reflecting the activities of L-type voltage-dependent Ca²⁺ channels. Whole-cell recordings also demonstrated increased high-voltage-activated Ca²⁺ currents in bFGF-treated neurons, whereas low-voltage-activated Ca²⁺ currents remained unchanged. bFGF-stimulated increase in Ca²⁺ response was not observed in neurons treated with cycloheximide or actinomycin D, indicating that protein and RNA synthesis were required for this effect. Visualization using a fluorescent dihydropyridine analog revealed that bFGF-treated neurons expressed increased amounts of L-type Ca²⁺ channels on the cell body. In addition, bFGF-treated neurons acquired distinctive morphology of neurites that was characterized by markedly increased neuritic branching. The branching points in neurites were associated with clusters of L-type Ca²⁺ channels and resultant ``Ca²⁺ hotspots'' that showed large [Ca²⁺]_i increases in response to membrane depolarization. Concurrent application of nicardipine completely blocked the bFGF-stimulated increase in neuritic branching. Therefore, bFGF enhances the expression of functional L-type Ca²⁺ channels on the cell body and neurites of fetal hippocampal neurons, which may play an important role in the regulation of their differentiation and the establishment of their neurite morphology.

Key words: basic fibroblast growth factor; hippocampal neurons; neuronal development; calcium; voltage-dependent Ca²⁺ channel; expression; channel distribution; neurite branching

This article has been cited by other articles:

				S. Ohba, T. Ikeda, Y. Ikegaya, N. Nishiyama, N. Matsuki, and M. K. Yamada BDNF Locally Potentiates GABAergic Presynaptic Machineries: Target-selective Circuit Inhibition Cereb Cortex, March 1, 2005; 15(3): 291 - 298. [Abstract] [Full Text] [PDF]

				E. W. Dent, F. Tang, and K. Kalil Axon Guidance by Growth Cones and Branches: Common Cytoskeletal and Signaling Mechanisms Neuroscientist, October 1, 2003; 9(5): 343 - 353. [Abstract] [PDF]

				M. K. Yamada, K. Nakanishi, S. Ohba, T. Nakamura, Y. Ikegaya, N. Nishiyama, and N. Matsuki Brain-Derived Neurotrophic Factor Promotes the Maturation of GABAergic Mechanisms in Cultured Hippocampal Neurons J. Neurosci., September 1, 2002; 22(17): 7580 - 7585. [Abstract] [Full Text] [PDF]

				Y. Ikegaya, S. Matsuura, S. Ueno, A. Baba, M. K. Yamada, N. Nishiyama, and N. Matsuki beta -Amyloid Enhances Glial Glutamate Uptake Activity and Attenuates Synaptic Efficacy J. Biol. Chem., August 23, 2002; 277(35): 32180 - 32186. [Abstract] [Full Text] [PDF]

				N. Zhang and E. Townes-Anderson Regulation of Structural Plasticity by Different Channel Types in Rod and Cone Photoreceptors J. Neurosci., August 15, 2002; 22(16): 7065 - 7079. [Abstract] [Full Text] [PDF]

				P. Mariot, K. Vanoverberghe, N. Lalevee, M. F. Rossier, and N. Prevarskaya Overexpression of an alpha 1H (Cav3.2) T-type Calcium Channel during Neuroendocrine Differentiation of Human Prostate Cancer Cells J. Biol. Chem., March 22, 2002; 277(13): 10824 - 10833. [Abstract] [Full Text] [PDF]

				G. Szebenyi, E. W. Dent, J. L. Callaway, C. Seys, H. Lueth, and K. Kalil Fibroblast Growth Factor-2 Promotes Axon Branching of Cortical Neurons by Influencing Morphology and Behavior of the Primary Growth Cone J. Neurosci., June 1, 2001; 21(11): 3932 - 3941. [Abstract] [Full Text] [PDF]

				J. M. Schjott and M. R. Plummer Sustained Activation of Hippocampal Lp-Type Voltage-Gated Calcium Channels by Tetanic Stimulation J. Neurosci., July 1, 2000; 20(13): 4786 - 4797. [Abstract] [Full Text] [PDF]

				A. L. Boxer, H. Moreno, B. Rudy, and E. B. Ziff FGF-2 Potentiates Ca2+-Dependent Inactivation of NMDA Receptor Currents in Hippocampal Neurons J Neurophysiol, December 1, 1999; 82(6): 3367 - 3377. [Abstract] [Full Text] [PDF]

				B. Hivert, S. Luvisetto, A. Navangione, A. Tottene, and D. Pietrobon Anomalous L-Type Calcium Channels of Rat Spinal Motoneurons J. Gen. Physiol., May 1, 1999; 113(5): 679 - 694. [Abstract] [Full Text] [PDF]

				H Meinhardt Orientation of chemotactic cells and growth cones: models and mechanisms J. Cell Sci., January 9, 1999; 112(17): 2867 - 2874. [Abstract] [PDF]

				J. R. McLeod Jr., M. Shen, D. J. Kim, and S. A. Thayer Neurotoxicity Mediated by Aberrant Patterns of Synaptic Activity Between Rat Hippocampal Neurons in Culture J Neurophysiol, November 1, 1998; 80(5): 2688 - 2698. [Abstract] [Full Text] [PDF]

				R Chauhan-Patel and A E Spruce Differential regulation of potassium currents by FGF-1 and FGF-2 in embryonic Xenopus laevis myocytes J. Physiol., October 1, 1998; 512(1): 109 - 118. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help