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Volume 16, Number 21,
Issue of November 1, 1996
pp. 6703-6712
Copyright ©1996 Society for Neuroscience
Ryanodine-Sensitive Component of Calcium Transients Evoked by
Nerve Firing at Presynaptic Nerve Terminals
Received June 14, 1996; revised Aug. 5, 1996; accepted Aug. 13, 1996.
Yan-yi Peng
Department of Pharmacological and Physiological Sciences,
Committees on Neurobiology and Cell Physiology, The University of
Chicago, Chicago, Illinois 60637
Whether Ca2+ released from stores within the
presynaptic nerve terminals also contributes to the Ca2+
elevation evoked by action potentials was tested in intact bullfrog
sympathetic ganglia. Intraterminal Ca2+ transients
( [Ca2+]i) were evoked by electrical shocks
to the presynaptic nerves at 20 Hz and were monitored by fura-2
fluorimetry. Ca2+ released through intraterminal
ryanodine-sensitive channels accounted for 46% of the peak
Ca2+ elevation. Moreover, in half of the terminals when
intraterminal release was blocked by ryanodine,
[Ca2+]i reached a plateau at 200 ± 24 nM. Because 20 Hz is a frequency favorable for the
release of a neuropeptide, luteinizing hormone releasing hormone (LHRH)
from these presynaptic nerve terminals, and because the threshold level
for LHRH release is 186 nM, intraterminal Ca2+
release during nerve firing is likely to play a major role in
regulating LHRH release. The intraterminal ryanodine channels were
facilitated by caffeine as in other tissue. The releasable
ryanodine-sensitive store could elevate the intraterminal
[Ca2+] by an amount as high as 1.6 µM at a
rate as fast as 250 nM/sec. The store could be refilled
within 100 sec after a maximal discharge of its content by 20 Hz
firing. Oscillation of [Ca2+]i evoked by 20 Hz nerve firing occurred in normal Ringer solution, in ryanodine, and
in caffeine with a periodicity of ~10 sec. Besides the facilitatory
effects on the ryanodine-sensitive channels, caffeine also had
inhibitory effects on [Ca2+]i via its
action on a different process.
Key words:
synaptic transmission;
presynaptic calcium dynamics;
fura-2 fluorimetry;
neuropeptide release;
ryanodine channel;
intraterminal Ca2+ store;
ryanodine;
caffeine
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