 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 16, Number 21, Issue of November 1, 1996 pp. 6896-6907
Copyright ©1996 Society for NeuroscienceDifferential Survival of Cajal-Retzius Cells in Organotypic Cultures of Hippocampus and Neocortex
Received March 18, 1996; revised Aug. 1, 1996; accepted Aug. 12, 1996.
José A. Del Río1, 2, Bernd Heimrich2, Hans Supèr1, Víctor Borrell1, Michael Frotscher2, and Eduardo Soriano1 1 Department of Animal and Plant Cell Biology, Faculty of Biology, University of Barcelona, 08028 Barcelona, Spain, and 2 Institute of Anatomy, University of Freiburg, D-79001 Freiburg, Germany
Cajal-Retzius (CR) cells are transient, pioneer neurons of layer I of the cortex that are believed to play essential roles in corticogenesis, e.g., in neuronal migration and synaptogenesis. Here we have used calretinin immunostaining to study the characteristics, survival, and fate of CR cells in single organotypic slice cultures of mouse neocortex and hippocampus deprived of their extrinsic afferents. In neocortical explants, CR cells were observed after 1-3 d in vitro (DIV), but they disappeared after 5-7 DIV, which is similar to their time of degeneration in vivo. The disappearance of CR cells in neocortical slices was prevented by incubation with tetrodotoxin and the glutamate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3,-dione but not by 2-amino-5-phosphonopentanoic acid, suggesting that neuronal activity and non-NMDA glutamate receptors may trigger CR cell death in the neocortex.
In contrast to the situation in vivo, in which many hippocampal CR cells disappear at approximately the third postnatal week, CR cells survived in single hippocampal cultures after long incubation times (31 DIV), with their morphology essentially unaltered. In contrast, fewer CR cells were found when hippocampal slices were cocultured with explants from the entorhinal cortex. Because CR cells are transient synaptic targets for entorhinohippocampal afferents, these findings suggest a role for entorhinal afferents in the degeneration of CR cells in the hippocampus. In conclusion, this study shows different survival properties of CR cells in organotypic slice cultures of hippocampus and neocortex, and it suggests that different mechanisms are involved in the regulation of the process of naturally occurring CR cell death in the two cortical regions.
Key words: Cajal-Retzius cells; cortical development; neuronal death; neuronal activity; calretinin; organotypic cultures; mouse
This article has been cited by other articles:
V. Beglopoulos, M. Montag-Sallaz, A. Rohlmann, K. Piechotta, M. Ahmad, D. Montag, and M. Missler
Neurexophilin 3 Is Highly Localized in Cortical and Cerebellar Regions and Is Functionally Important for Sensorimotor Gating and Motor Coordination
Mol. Cell. Biol., August 15, 2005; 25(16): 7278 - 7288.
[Abstract] [Full Text] [PDF]
W. J. Moody and M. M. Bosma
Ion Channel Development, Spontaneous Activity, and Activity-Dependent Development in Nerve and Muscle Cells
Physiol Rev, July 1, 2005; 85(3): 883 - 941.
[Abstract] [Full Text] [PDF]
V. Martinez-Cerdeno, M. J. Galazo, C. Cavada, and F. Clasca
Reelin Immunoreactivity in the Adult Primate Brain: Intracellular Localization in Projecting and Local Circuit Neurons of the Cerebral Cortex, Hippocampus and Subcortical Regions
Cereb Cortex, December 1, 2002; 12(12): 1298 - 1311.
[Abstract] [Full Text] [PDF]
S. Nishikawa, S. Goto, T. Hamasaki, K. Yamada, and Y. Ushio
Involvement of Reelin and Cajal-Retzius Cells in the Developmental Formation of Vertical Columnar Structures in the Cerebral Cortex: Evidence from the Study of Mouse Presubicular Cortex
Cereb Cortex, October 1, 2002; 12(10): 1024 - 1030.
[Abstract] [Full Text] [PDF]
G. Radnikow, D. Feldmeyer, and J. Lubke
Axonal Projection, Input and Output Synapses, and Synaptic Physiology of Cajal-Retzius Cells in the Developing Rat Neocortex
J. Neurosci., August 15, 2002; 22(16): 6908 - 6919.
[Abstract] [Full Text] [PDF]
Y. Chen, R. A. Bender, M. Frotscher, and T. Z. Baram
Novel and Transient Populations of Corticotropin-Releasing Hormone-Expressing Neurons in Developing Hippocampus Suggest Unique Functional Roles: A Quantitative Spatiotemporal Analysis
J. Neurosci., September 15, 2001; 21(18): 7171 - 7181.
[Abstract] [Full Text] [PDF]
E. Forster, S. Zhao, and M. Frotscher
Hyaluronan-associated adhesive cues control fiber segregation in the hippocampus
Development, August 1, 2001; 128(15): 3029 - 3039.
[Abstract] [Full Text] [PDF]
T. Hamasaki, S. Goto, S. Nishikawa, and Y. Ushio
Early-generated Preplate Neurons in the Developing Telencephalon: Inward Migration into the Developing Striatum
Cereb Cortex, May 1, 2001; 11(5): 474 - 484.
[Abstract] [Full Text] [PDF]
T. N. Behar, A. E. Schaffner, C. A. Scott, C. L. Greene, and J. L. Barker
GABA Receptor Antagonists Modulate Postmitotic Cell Migration in Slice Cultures of Embryonic Rat Cortex
Cereb Cortex, September 1, 2000; 10(9): 899 - 909.
[Abstract] [Full Text] [PDF]
H. Super, J. A. Del Rio, A. Martinez, P. Perez-Sust, and E. Soriano
Disruption of Neuronal Migration and Radial Glia in the Developing Cerebral Cortex Following Ablation of Cajal-Retzius Cells
Cereb Cortex, June 1, 2000; 10(6): 602 - 613.
[Abstract] [Full Text] [PDF]
J.-M. Mienville
Feature Article: Cajal-Retzius Cell Physiology: Just in Time to Bridge the 20th Century
Cereb Cortex, December 1, 1999; 9(8): 776 - 782.
[Abstract] [Full Text] [PDF]
M. Alvarez-Dolado, M. Ruiz, J. A. Del Rio, S. Alcantara, F. Burgaya, M. Sheldon, K. Nakajima, J. Bernal, B. W. Howell, T. Curran, et al.
Thyroid Hormone Regulates reelin and dab1 Expression During Brain Development
J. Neurosci., August 15, 1999; 19(16): 6979 - 6993.
[Abstract] [Full Text] [PDF]
J.-M. Mienville and C. Pesold
Low Resting Potential and Postnatal Upregulation of NMDA Receptors May Cause Cajal-Retzius Cell Death
J. Neurosci., March 1, 1999; 19(5): 1636 - 1646.
[Abstract] [Full Text] [PDF]
V. Borrell, J. A. Del Rio, S. Alcantara, M. Derer, A. Martinez, G. D'Arcangelo, K. Nakajima, K. Mikoshiba, P. Derer, T. Curran, et al.
Reelin Regulates the Development and Synaptogenesis of the Layer-Specific Entorhino-Hippocampal Connections
J. Neurosci., February 15, 1999; 19(4): 1345 - 1358.
[Abstract] [Full Text] [PDF]
S. Alcantara, M. Ruiz, G. D'Arcangelo, F. Ezan, L. de Lecea, T. Curran, C. Sotelo, and E. Soriano
Regional and Cellular Patterns of reelin mRNA Expression in the Forebrain of the Developing and Adult Mouse
J. Neurosci., October 1, 1998; 18(19): 7779 - 7799.
[Abstract] [Full Text] [PDF]
C. Pesold, F. Impagnatiello, M. G. Pisu, D. P. Uzunov, E. Costa, A. Guidotti, and H. J. Caruncho
Reelin is preferentially expressed in neurons synthesizing gamma -aminobutyric acid in cortex and hippocampus of adult rats
PNAS, March 17, 1998; 95(6): 3221 - 3226.
[Abstract] [Full Text] [PDF]
E Forster, C Kaltschmidt, J Deng, H Cremer, T Deller, and M Frotscher
Lamina-specific cell adhesion on living slices of hippocampus
Development, January 9, 1998; 125(17): 3399 - 3410.
[Abstract] [PDF]
K. Ceranik, R. Bender, J. R. P. Geiger, H. Monyer, P. Jonas, M. Frotscher, and J. Lubke
A Novel Type of GABAergic Interneuron Connecting the Input and the Output Regions of the Hippocampus
J. Neurosci., July 15, 1997; 17(14): 5380 - 5394.
[Abstract] [Full Text] [PDF]
S. Alcantara, J. Frisen, J. A. del Rio, E. Soriano, M. Barbacid, and I. Silos-Santiago
TrkB Signaling Is Required for Postnatal Survival of CNS Neurons and Protects Hippocampal and Motor Neurons from Axotomy-Induced Cell Death
J. Neurosci., May 15, 1997; 17(10): 3623 - 3633.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|