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Previous Article | Next Article
Volume 16, Number 22, Issue of November 15, 1996 pp. 7171-7181
Copyright ©1996 Society for NeuroscienceTranslational Machinery in Dendrites of Hippocampal Neurons in Culture
Received June 14, 1996; revised Aug. 30, 1996; accepted Sept. 4, 1996.
Henri Tiedge1 and Jürgen Brosius2 1 Departments of Pharmacology and Neurology, State University of New York Health Science Center at Brooklyn, Brooklyn, New York 11203, and 2 Institute for Experimental Pathology, Center for Molecular Biology of Inflammation, University of Münster, D-48129 Münster, Germany
In neurons, several mRNAs are selectively delivered to dendritic domains where they are presumably translated by local protein synthetic machinery. Although electron microscopy has identified polyribosomes in dendrites, in particular in postsynaptic dendritic compartments, the functional composition of the local protein synthetic apparatus and the scope of its translational capacity have not been analyzed. To ascertain the translational competence of dendrites, we have probed hippocampal neurons in primary culture for various integral and associated factors of the translational apparatus. We report here that dendrites of such neurons are equipped with a spectrum of translational machinery components, including ribosomes, tRNAs, initiation and elongation factors, and elements of the cotranslational signal recognition mechanism. These components are differentially and nonuniformly distributed in dendritic arbors. Their dendritic location illustrates the soma-independent potential of dendrites to synthesize selected proteins in local domains.
Key words: dendrite; dendritic RNA; hippocampal neurons; local protein synthesis; ribosomes; translational apparatus; immunocytochemistry; in situ hybridization
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