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Volume 16, Number 24, Issue of December 15, 1996 pp. 7868-7879
Copyright ©1996 Society for Neuroscience

A Drosophila Calcium Channel 1 Subunit Gene Maps to a Genetic Locus Associated with Behavioral and Visual Defects

Received July 15, 1996; revised Sept. 11, 1996; accepted Sept. 30, 1996.

Lee A. Smith¹, XinJing Wang², Alexandre A. Peixoto¹, Eric K. Neumann¹, Linda M. Hall², and Jeffrey C. Hall¹

¹ Department of Biology, Brandeis University, Waltham, Massachusetts 02254, and ² Department of Biochemical Pharmacology, State University of New York at Buffalo, Buffalo, New York 14260

We have cloned cDNAs that encode a complete open reading frame for a calcium channel 1 subunit from Drosophila melanogaster. The deduced 1851 amino acid protein belongs to the superfamily of voltage-gated sodium and calcium channels. Phylogenetic analysis shows that the sequence of this subunit is relatively distant from sodium channel  subunits and most similar to genes encoding the A, B, and E isoforms of calcium channel 1 subunits. To indicate its similarity to this subfamily of vertebrate isoforms, we name this protein Dmca1A, for Drosophila melanogaster calcium channel 1 subunit, type A. Northern blot analysis detected a single 10.5 kb transcript class that is regulated developmentally, with expression peaks in the first larval instar, midpupal, and late pupal stages. In late-stage embryos, Dmca1A is expressed preferentially in the nervous system. Variant transcripts are generated by alternative splicing. In addition, single nucleotide variations between cDNAs and genomic sequence are consistent with RNA editing. Dmca1A maps to a chromosomal region implicated in, and is the likely candidate for, the gene involved in the generation of behavioral, physiological, and lethal phenotypes of the cacophony, nightblind-A, and lethal(1)L13 mutants.

Key words: cDNA sequence; RNA editing; alternative splicing; phenylalkylamine binding site; chromosome aberrations; vital gene

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