QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (49) Citing Articles via Google Scholar Google Scholar Articles by Hajihosseini, M. Articles by Dubois-Dalcq, M. Search for Related Content PubMed PubMed Citation Articles by Hajihosseini, M. Articles by Dubois-Dalcq, M. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

Volume 16, Number 24, Issue of December 15, 1996 pp. 7981-7994
Copyright ©1996 Society for Neuroscience

Origin of Oligodendrocytes within the Human Spinal Cord

Received Aug. 5, 1996; revised Sept. 26, 1996; accepted Sept. 30, 1996.

Mohammad Hajihosseini, To Nam Tham, and Monique Dubois-Dalcq

Unité de Neurovirologie et Régénération du Système Nerveux, Institut Pasteur, 75015 Paris, France

To determine the time and site of origin of the oligodendrocyte lineage in the developing human spinal cord, we have examined tissues from 45 to 83 d postconception (dpc) using sets of probes and antibodies recognizing oligodendrocyte-specific glycolipids, transcripts, and proteins. We found that two clusters of oligodendrocyte precursors appear on or before 45 dpc on each side of the cord ventral ependyma above the floor plate. These precursors express glycolipids recognized by the O4 and Rmab antibodies, platelet-derived growth factor -receptor, myelin basic protein (MBP), and 2, 3-cyclic nucleotide 3 phosphodiesterase as well as MBP and proteolipid transcripts. Expression of the morphogen sonic hedgehog was detected in the floor plate at 45 dpc and decreased at 58 dpc. During this period, oligodendrocyte precursors emerged in the ventral and lateral region of the forming white matter, a process occurring first in cervical and later in lumbar cord. The majority of O4⁺ cells express the proliferating cell nuclear antigen (PCNA), and their pattern of dispersion suggests that these cells progressively populate the lateral and dorsal cord regions. Oligodendrocytes expressing galactocerebroside appeared at 53 dpc and did not express PCNA. Oligodendrocyte precursors were detected in dorsal cord regions at 74 dpc and at 83 dpc when myelination started in the ventral roots. Thus, oligodendrocyte precursors expressing myelin transcripts and proteins emerge in the ventral region of the embryonic cord several weeks before myelination.

Key words: oligodendrocyte origin; developing human spinal cord; myelin gene transcripts; in situ hybridization; immunolabeling of myelin specific glycolipids and proteins; platelet-derived growth factor  receptor

This article has been cited by other articles:

				J. Samanta and J. A. Kessler Interactions between ID and OLIG proteins mediate the inhibitory effects of BMP4 on oligodendroglial differentiation Development, September 1, 2004; 131(17): 4131 - 4142. [Abstract] [Full Text] [PDF]

				A. A. Jarjour, C. Manitt, S. W. Moore, K. M. Thompson, S.-J. Yuh, and T. E. Kennedy Netrin-1 Is a Chemorepellent for Oligodendrocyte Precursor Cells in the Embryonic Spinal Cord J. Neurosci., May 1, 2003; 23(9): 3735 - 3744. [Abstract] [Full Text] [PDF]

				S. Mekki-Dauriac, E. Agius, P. Kan, and P. Cochard Bone morphogenetic proteins negatively control oligodendrocyte precursor specification in the chick spinal cord Development, March 13, 2003; 129(22): 5117 - 5130. [Abstract] [Full Text] [PDF]

				N. Spassky, K. Heydon, A. Mangatal, A. Jankovski, C. Olivier, F. Queraud-Lesaux, C. Goujet-Zalc, J. L. Thomas, and B. Zalc Sonic hedgehog-dependent emergence of oligodendrocytes in the telencephalon: evidence for a source of oligodendrocytes in the olfactory bulb that is independent of PDGFR{alpha} signaling Development, December 15, 2001; 128(24): 4993 - 5004. [Abstract] [Full Text] [PDF]

				S. A. Back, N. L. Luo, N. S. Borenstein, J. M. Levine, J. J. Volpe, and H. C. Kinney Late Oligodendrocyte Progenitors Coincide with the Developmental Window of Vulnerability for Human Perinatal White Matter Injury J. Neurosci., February 15, 2001; 21(4): 1302 - 1312. [Abstract] [Full Text] [PDF]

				K. J. Chandross, R. I. Cohen, P. Paras Jr, M. Gravel, P. E. Braun, and L. D. Hudson Identification and Characterization of Early Glial Progenitors Using a Transgenic Selection Strategy J. Neurosci., January 15, 1999; 19(2): 759 - 774. [Abstract] [Full Text] [PDF]

				G. Wolswijk Chronic Stage Multiple Sclerosis Lesions Contain a Relatively Quiescent Population of Oligodendrocyte Precursor Cells J. Neurosci., January 15, 1998; 18(2): 601 - 609. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help