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Journal of Neuroscience, Vol 16, 1163-1172, Copyright © 1996 by Society for Neuroscience
Analysis of integrin expression on oligodendrocytes during axo-glial interaction by using rat-mouse xenocultures
CE Shaw, R Milner, AS Compston and C ffrench-Constant
University of Cambridge Neurology Unit, Addenbrookes Hospital, United Kingdom.
To analyze the expression of cell-surface molecules on neurons or glia
during myelination, we have developed a xenotypic coculture system in which
mouse oligodendrocytes interact with rat dorsal root ganglion neurons. The
axo-glial interactions in these cultures promote oligodendrocyte precursor
cell proliferation, survival, and differentiation as in vivo, thus
supporting the validity of the xenocultures as a model system to study
myelination in which the molecules on the neurons or the glia can be
distinguished using species- specific antibodies. We examined the
expression of integrins, the major family of cell-surface extracellular
matrix receptors, on oligodendrocytes during the early stages of
myelination and found that, unlike Schwann cells, oligodendrocytes do not
express alpha 6 beta 4 in association with myelin sheath formation. The
pattern of integrin expression observed on oligodendrocytes in these
cultures is similar to that seen in oligodendrocytes that differentiate in
purified cultures, and it comprises alpha 6 beta 1, alpha v beta 5, and an
as yet uncharacterized alpha v-associated beta subunit of 80 kDa. Changes
in integrin expression associated with differentiation, therefore, do not
depend on axonal contact, and beta 4 is not required for myelin sheath
formation, although its expression may contribute to the individual
properties of oligodendrocytes and Schwann cells.
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