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Journal of Neuroscience, Vol 16, 1689-1701, Copyright © 1996 by Society for Neuroscience
Lobster shal: comparison with Drosophila shal and native potassium currents in identified neurons
DJ Baro, LM Coniglio, CL Cole, HE Rodriguez, JK Lubell, MT Kim and RM Harris- Warrick
Section of Neurobiology and Behavior, Cornell University, Ithaca, New York 14853, USA.
The transient potassium (K+) current, or A-current (IA), plays an essential
role in shaping the firing properties of identified neurons in the 14-cell
pyloric network in the stomatogastric ganglion of the spiny lobster,
Panulirus interruptus. The different cells in the pyloric network have
distinct IAs. To begin to understand the molecular basis for IA
heterogeneity, we examined the relationship between the Panulirus shal
current, the IAs in the lateral pyloric (LP) and pyloric dilator (PY)
cells, and the Drosophila shal current. After isolating a complete open
reading frame for lobster shal 1, which shows significant sequence homology
to the fly, mouse, and rat shal homologs, we used a single-cell reverse
transcription polymerase chain reaction method to demonstrate that the shal
1 gene was expressed in the LP and PY cells. Next, we compared the lobster
shal 1 current generated in a Xenopus oocyte expression system to the IAs
in the LP and PY neurons as well as to the Drosophila shal current in
Xenopus oocytes. While the transient K+ lobster shal 1 current was similar
to the IAs in pyloric neurons, a detailed comparison shows that they are
not identical and differ in kinetic and voltage-dependent parameters. The
highly homologous lobster and fly shal genes also produce currents with
some significant similarities and differences in an oocyte expression
system.
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