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Journal of Neuroscience, Vol 16, 1827-1835, Copyright © 1996 by Society for Neuroscience
Tissue-specific expression of a Ca(2+)-activated K+ channel is controlled by multiple upstream regulatory elements
R Brenner, TO Thomas, MN Becker and NS Atkinson
Department of Zoology, University of Texas at Austin 78712-1064, USA.
The electrical properties of a cell are produced by the complement of ion
channels that it expresses. To understand how ion-channel gene expression
is regulated, we are studying the tissue-specific regulation of the
slowpoke (slo) Ca(2+)-activated K+ channel gene. This gene is expressed in
the central and peripheral nervous system, in midgut and tracheal cells,
and in the musculature of Drosophila melanogaster. The entire
transcriptional control region has been cloned previously and shown to
reproduce the tissue and developmental expression pattern of the endogenous
gene. Here we demonstrate that s/o has at least four promoters distributed
over approximately 4.5 kb of DNA. Promoter C1 and C1c display a TATA
box-like sequence at the appropriate distance from the transcription start
site. Promoters C1b and C2, however, are TATA- less promoters. C1, C1b, and
C1c transcripts differ in their leader sequence but share a common
translation start site. C2 transcripts incorporate a new translation start
site that appends 17 amino acids to the N terminus of the encoded protein.
Deletion analysis was used to identify sequences important for
tissue-specific expression. We used a transgenic in vivo expression system
in which all tissues and developmental stages can be assayed easily. Six
nested deletions were transformed into Drosophila, and the expression
pattern was determined using a lacZ reporter in both dissected tissues and
sectioned animals. We have identified different sequences required for
expression in the CNS, midgut, tracheal cells, and muscle.
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