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Journal of Neuroscience, Vol 16, 2127-2140, Copyright © 1996 by Society for Neuroscience
Selective clustering of GABA(A) and glycine receptors in the mammalian retina
P Koulen, M Sassoe-Pognetto, U Grunert and H Wassle
Max-Planck-Institut fur Hirnforschung, Frankfurt, Germany.
Molecular cloning has revealed a multiplicity of neurotransmitter receptor
isoforms with different subunit compositions. Additionally, there is
growing evidence that such receptors are clustered at postsynaptic sites of
neurons. Thus, the questions arise whether individual neurons express
different receptor isoforms and, if so, whether different isoforms are
present within the same cluster or are aggregated at distinct postsynaptic
sites. We have studied with immunofluorescence methods and antibodies that
recognize specific subunits the distribution of glycine and GABA(A)
receptors in mammalian retinae. Alpha ganglion cells were injected in rat
or rabbit retinae with a fluorescent marker and then immunostained for
receptor localization. Clusters of glycine receptors and clusters of the
alpha1, and alpha2, alpha3, and gamma2 subunits of the GABA(A) receptor
were found on the somatodendritic membranes of Alpha ganglion cells.
Double- immunofluorescence experiments with different combinations of the
subunit-specific antibodies showed that the alpha1, alpha2, and alpha3
subunits of the GABA(A) receptor are not colocalized within the same
clusters. These results indicate that an individual neuron can express
several isoforms of the GABA(A) receptor and that these different isoforms
are aggregated at distinct postsynaptic sites. This suggests individual
sorting mechanisms of GABAa receptors at GABAergic synapses.
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