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Next Article 
Journal of Neuroscience, Vol 16, 2157-2163, Copyright © 1996 by Society for Neuroscience
Interaction between the C terminus of NMDA receptor subunits and multiple members of the PSD-95 family of membrane-associated guanylate kinases
M Niethammer, E Kim and M Sheng
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02114, USA.
Selective concentration and anchoring of ionotropic receptors at the
synapse is essential for neuronal signaling. Little is known about the
molecules that mediate receptor clustering in the CNS. With use of the
yeast two-hybrid system to screen a rat brain cDNA library and by in vitro
binding assays, we have identified an interaction between NMDA receptor
subunits 2A and 2B (NR2A and NR2B) and three distinct members of the
PSD-95/SAP90 family of membrane-associated putative guanylate kinases. The
interaction is mediated by binding of the C terminus of the NMDA receptor
subunits to the first two PDZ (also known as GLGF or DHR) domains of
PSD-95/SAP90, an abundant synaptic protein associated with the membrane
cytoskeleton. PSD-95 is also known to bind and cluster Shaker-type
voltage-gated K+ channels. Similarities between the C-termini of NR2
subunits and K+ channels suggest a common C-terminal binding motif for PDZ
domains. These data suggest that PDZ domains can function as modules for
protein-protein interactions. Members of the PSD-95 family might serve to
anchor NMDA receptors to the submembrane cytoskeleton and aid in the
assembly of signal transduction complexes at postsynaptic sites.
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|
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|
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|
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281(31):
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|
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|
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103(12):
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|
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11061 - 11070.
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|
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25(41):
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|
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280(29):
26992 - 26996.
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|
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|
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|
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|
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11(1):
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|
|
|
|
|
|
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J. Cao, J. I. Viholainen, C. Dart, H. K. Warwick, M. L. Leyland, and M. J. Courtney
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168(1):
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|
|
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|
|
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24(49):
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[Full Text]
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|
|
|
|
|
|
|
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279(46):
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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24(45):
10138 - 10148.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Chung, Y. H. Huang, L.-F. Lau, and R. L. Huganir
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J. Neurosci.,
November 10, 2004;
24(45):
10248 - 10259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gupta, R. Singh, P. Datta, Z. Zhang, C. Orr, Z. Lu, G. DuBois, A. S. Zervos, M. H. Meisler, S. M. Srinivasula, et al.
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279(44):
45844 - 45854.
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[Full Text]
[PDF]
|
|
|
|
|
|
|
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24(33):
7292 - 7304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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10(4):
325 - 336.
[Abstract]
[PDF]
|
|
|
|
|
|
|
D. Leonoudakis, L. R. Conti, S. Anderson, C. M. Radeke, L. M. M. McGuire, M. E. Adams, S. C. Froehner, J. R. Yates III, and C. A. Vandenberg
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279(21):
22331 - 22346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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279(20):
21003 - 21011.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Seeber, A. Humeny, M. Herkert, T. Rau, T. Eschenhagen, and C.-M. Becker
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J. Biol. Chem.,
May 14, 2004;
279(20):
21062 - 21068.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. McFEETERS and R. E. OSWALD
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March 1, 2004;
18(3):
428 - 438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Bresler, M. Shapira, T. Boeckers, T. Dresbach, M. Futter, C. C. Garner, K. Rosenblum, E. D. Gundelfinger, and N. E. Ziv
Postsynaptic Density Assembly Is Fundamentally Different from Presynaptic Active Zone Assembly
J. Neurosci.,
February 11, 2004;
24(6):
1507 - 1520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. A. Morabito, M. Sheng, and L.-H. Tsai
Cyclin-Dependent Kinase 5 Phosphorylates the N-Terminal Domain of the Postsynaptic Density Protein PSD-95 in Neurons
J. Neurosci.,
January 28, 2004;
24(4):
865 - 876.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Ehrlich and R. Malinow
Postsynaptic Density 95 controls AMPA Receptor Incorporation during Long-Term Potentiation and Experience-Driven Synaptic Plasticity
J. Neurosci.,
January 28, 2004;
24(4):
916 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bhattacharjee, W. J. Joiner, M. Wu, Y. Yang, F. J. Sigworth, and L. K. Kaczmarek
Slick (Slo2.1), a Rapidly-Gating Sodium-Activated Potassium Channel Inhibited by ATP
J. Neurosci.,
December 17, 2003;
23(37):
11681 - 11691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Li, Y. Otsu, T. H. Murphy, and L. A. Raymond
Developmental Decrease in NMDA Receptor Desensitization Associated with Shift to Synapse and Interaction with Postsynaptic Density-95
J. Neurosci.,
December 3, 2003;
23(35):
11244 - 11254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Jiang, D. Mu, R. A. Sheldon, D. V. Glidden, and D. M. Ferriero
Neonatal Hypoxia-Ischemia Differentially Upregulates MAGUKs and Associated Proteins in PSD-93-Deficient Mouse Brain
Stroke,
December 1, 2003;
34(12):
2958 - 2963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Gardoni, D. Mauceri, C. Fiorentini, C. Bellone, C. Missale, F. Cattabeni, and M. D. Luca
CaMKII-dependent Phosphorylation Regulates SAP97/NR2A Interaction
J. Biol. Chem.,
November 7, 2003;
278(45):
44745 - 44752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Matsuda, M. Fletcher, Y. Kamiya, and M. Yuzaki
Specific Assembly with the NMDA Receptor 3B Subunit Controls Surface Expression and Calcium Permeability of NMDA Receptors
J. Neurosci.,
November 5, 2003;
23(31):
10064 - 10073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fang, Y.-X. Tao, F. He, M. Zhang, C. F. Levine, P. Mao, F. Tao, C.-L. Chou, S. Sadegh-Nasseri, and R. A. Johns
Synaptic PDZ Domain-mediated Protein Interactions Are Disrupted by Inhalational Anesthetics
J. Biol. Chem.,
September 19, 2003;
278(38):
36669 - 36675.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Nourry, S. G. N. Grant, and J.-P. Borg
PDZ Domain Proteins: Plug and Play!
Sci. Signal.,
April 22, 2003;
2003(179):
re7 - re7.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kitano, Y. Yamazaki, K. Kimura, T. Masukado, Y. Nakajima, and S. Nakanishi
Tamalin Is a Scaffold Protein That Interacts with Multiple Neuronal Proteins in Distinct Modes of Protein-Protein Association
J. Biol. Chem.,
April 18, 2003;
278(17):
14762 - 14768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Fukaya, A. Kato, C. Lovett, S. Tonegawa, and M. Watanabe
Retention of NMDA receptor NR2 subunits in the lumen of endoplasmic reticulum in targeted NR1 knockout mice
PNAS,
April 15, 2003;
100(8):
4855 - 4860.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Beumer, H. J. G. Matthies, A. Bradshaw, and K. Broadie
Integrins regulate DLG/FAS2 via a CaM kinase II-dependent pathway to mediate synapse elaboration and stabilization during postembryonic development
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March 9, 2003;
129(14):
3381 - 3391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Okabe, T. Nakamura, Y. N. Nishimura, K. Kohu, S. Ohwada, Y. Morishita, and T. Akiyama
RICS, a Novel GTPase-activating Protein for Cdc42 and Rac1, Is Involved in the beta -Catenin-N-cadherin and N-Methyl-D-aspartate Receptor Signaling
J. Biol. Chem.,
March 7, 2003;
278(11):
9920 - 9927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. J. Skelton, M. F. T. Koehler, K. Zobel, W. L. Wong, S. Yeh, M. T. Pisabarro, J. P. Yin, L. A. Lasky, and S. S. Sidhu
Origins of PDZ Domain Ligand Specificity. STRUCTURE DETERMINATION AND MUTAGENESIS OF THE ERBIN PDZ DOMAIN
J. Biol. Chem.,
February 21, 2003;
278(9):
7645 - 7654.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Harvey-Girard and R. J. Dunn
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J Neurophysiol,
February 1, 2003;
89(2):
822 - 832.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. P. Guttmann, S. Sokol, D. L. Baker, K. L. Simpkins, Y. Dong, and D. R. Lynch
Proteolysis of the N-Methyl-D-Aspartate Receptor by Calpain in Situ
J. Pharmacol. Exp. Ther.,
September 1, 2002;
302(3):
1023 - 1030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Cai, S. K Coleman, K. Niemi, and K. Keinanen
Selective Binding of Synapse-associated Protein 97 to GluR-A alpha -Amino-5-hydroxy-3-methyl-4-isoxazole Propionate Receptor Subunit Is Determined by a Novel Sequence Motif
J. Biol. Chem.,
August 23, 2002;
277(35):
31484 - 31490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Roche, M. C. Packard, S. Moeckel-Cole, and V. Budnik
Regulation of Synaptic Plasticity and Synaptic Vesicle Dynamics by the PDZ Protein Scribble
J. Neurosci.,
August 1, 2002;
22(15):
6471 - 6479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Lim, D. D. Hall, and J. W. Hell
Selectivity and Promiscuity of the First and Second PDZ Domains of PSD-95 and Synapse-associated Protein 102
J. Biol. Chem.,
June 7, 2002;
277(24):
21697 - 21711.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Wong, E. W. Newell, D. G. M. Jugloff, O. T. Jones, and L. C. Schlichter
Cell Surface Targeting and Clustering Interactions between Heterologously Expressed PSD-95 and the Shal Voltage-gated Potassium Channel, Kv4.2
J. Biol. Chem.,
May 31, 2002;
277(23):
20423 - 20430.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. DeSouza, J. Fu, B. A. States, and E. B. Ziff
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J. Neurosci.,
May 1, 2002;
22(9):
3493 - 3503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. F. Sasaki, T. Rothe, L. S. Premkumar, S. Das, J. Cui, M. V. Talantova, H.-K. Wong, X. Gong, S. F. Chan, D. Zhang, et al.
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J Neurophysiol,
April 1, 2002;
87(4):
2052 - 2063.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Chen, C. Aoki, V. Mahadomrongkul, C. E. Gruber, G. J. Wang, R. Blitzblau, N. Irwin, and P. A. Rosenberg
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March 15, 2002;
22(6):
2142 - 2152.
[Abstract]
[Full Text]
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|
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J. Cell Sci.,
March 11, 2002;
114(18):
3219 - 3231.
[Abstract]
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|
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|
|
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|
D. R. Lynch and R. P. Guttmann
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