QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schlamp, C. L. Articles by Nickells, R. W. Search for Related Content PubMed PubMed Citation Articles by Schlamp, C. L. Articles by Nickells, R. W.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 16, 2164-2171, Copyright © 1996 by Society for Neuroscience

ARTICLE

Light and dark cause a shift in the spatial expression of a neuropeptide-processing enzyme in the rat retina

CL Schlamp and RW Nickells
Department of Ophthalmology, University of Wisconsin, Madison, 53792, USA.

Carboxypeptidase E (CPE, EC 3.4.17.10) is an essential enzyme in the post-translational processing of most neuroactive peptides. We have studied how the expression of CPE in the rat retina is modified in response to the light/dark cycle. Retinal CPE mRNA levels increase immediately after a change in lighting condition. After prolonged exposure to the dark or the light, however, CPE mRNA levels drop to comparable amounts. The increase CPE mRNA is most dramatic during the dark-to-light transition. Interestingly, during the same interval, CPE protein levels transiently decrease. This apparently contradictory change in mRNA and protein levels is attributable to a change in the spatial pattern of CPE expression in the retina in response to light and dark. Both in situ hybridization and indirect immunofluorescence studies indicate that CPE is expressed in photoreceptors in the dark. During light onset, CPE expression is rapidly induced in retinal ganglion cells, whereas expression in photoreceptors is reduced. This pattern is reversed when the animals are exposed to the dark. In contrast, CPE is apparently constitutively expressed in a subpopulation of cells in the inner nuclear layer in both the light and the dark. These changes in CPE expression are similar to light-induced changes in c-fos expression, suggesting that CPE may be a downstream target for Fos activation.

This article has been cited by other articles:

				X. Zhong, J. Ge, E. L. Smith III, and W. K. Stell Image Defocus Modulates Activity of Bipolar and Amacrine Cells in Macaque Retina Invest. Ophthalmol. Vis. Sci., July 1, 2004; 45(7): 2065 - 2074. [Abstract] [Full Text] [PDF]

				P. R. van Ginkel, R. L. Gee, R. L. Shearer, L. Subramanian, T. M. Walker, D. M. Albert, L. F. Meisner, B. C. Varnum, and A. S. Polans Expression of the Receptor Tyrosine Kinase Axl Promotes Ocular Melanoma Cell Survival Cancer Res., January 1, 2004; 64(1): 128 - 134. [Abstract] [Full Text] [PDF]

				C. Helfrich-Forster, M. Tauber, J. H. Park, M. Muhlig-Versen, S. Schneuwly, and A. Hofbauer Ectopic Expression of the Neuropeptide Pigment-Dispersing Factor Alters Behavioral Rhythms in Drosophila melanogaster J. Neurosci., May 1, 2000; 20(9): 3339 - 3353. [Abstract] [Full Text] [PDF]

				R. S. Smith, S. W. M. John, A. Zabeleta, M. T. Davisson, N. L. Hawes, and B. Chang The Bst locus on mouse chromosome 16 is associated with age-related subretinal neovascularization PNAS, February 29, 2000; 97(5): 2191 - 2195. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help