 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 2563-2573, Copyright © 1996 by Society for Neuroscience
Role of glutamic acid decarboxylase in the prepubertal inhibition of the luteinizing hormone releasing hormone release in female rhesus monkeys
D Mitsushima, F Marzban, LL Luchansky, AJ Burich, KL Keen, M Durning, TG Golos and E Terasawa
Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, 53715, USA.
To investigate further the role of GABA in the onset of puberty, this study
examines whether glutamic acid decarboxylase (GAD), the catalytic enzyme
for GABA synthesis, is involved in the suppression of luteinizing hormone
releasing hormone (LHRH) before puberty in rhesus monkeys. First, both
GAD67 and GAD65 mRNAs were detectable by reverse transcription-PCR analysis
in the preoptic area, medio-basal hypothalamus, posterior hypothalamic
area, and hippocampus of the monkey brain. Second, effects of antisense
oligodeoxynucleotides (D- oligos) for GAD67 and GAD65 mRNAs on LHRH release
were examined in conscious female rhesus monkeys at the prepubertal stage
using a push- pull perfusion method. The GAD67 or GAD65 antisense D-oligos
or scrambled D-oligos were infused directly into the stalk-median eminence.
Both the GAD67 and the GAD65 antisense D-oligos induced a large and prompt
increase in LHRH release, whereas the scrambled D- oligos did not induce
any significant effect. The results suggest that the removal of GABA
inhibition by interfering with GAD synthesis is effective in increasing
LHRH release in prepubertal monkeys. Third, the specificity of the
antisense D-oligos on GAD levels was examined by incubating basal
hypothalami with D-oligos in vitro and subsequent Western blot analysis.
The antisense D-oligos consistently decreased the proteins GAD67 and GAD65
compared with respective control D-oligos. We conclude that the decrease of
tonic GABAergic inhibition and maturational changes in GAD synthesis may be
critical factors for the onset of puberty in nonhuman primates.
This article has been cited by other articles:
|
|
|
|
 
S. Constantin and S. Wray
Gonadotropin-Releasing Hormone-1 Neuronal Activity Is Independent of Cyclic Nucleotide-Gated Channels
Endocrinology,
January 1, 2008;
149(1):
279 - 290.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Ojeda, A. Lomniczi, C. Mastronardi, S. Heger, C. Roth, A.-S. Parent, V. Matagne, and A. E. Mungenast
Minireview: The Neuroendocrine Regulation of Puberty: Is the Time Ripe for a Systems Biology Approach?
Endocrinology,
March 1, 2006;
147(3):
1166 - 1174.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A.-S. Parent, M.-C. Lebrethon, A. Gerard, and J.-P. Bourguignon
Factors Accounting for Perinatal Occurrence of Pulsatile Gonadotropin-Releasing Hormone Secretion In Vitro in Rats
Biol Reprod,
January 1, 2005;
72(1):
143 - 149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Suter
The Ontogeny of Pulsatile Growth Hormone Secretion and Its Temporal Relationship to the Onset of Puberty in the Agonadal Male Rhesus Monkey (Macaca mulatta)
J. Clin. Endocrinol. Metab.,
May 1, 2004;
89(5):
2275 - 2280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. M. Plant and M. L. Barker-Gibb
Neurobiological mechanisms of puberty in higher primates
Hum. Reprod. Update,
January 1, 2004;
10(1):
67 - 77.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Qiu, M. A. Bosch, S. C. Tobias, D. K. Grandy, T. S. Scanlan, O. K. Ronnekleiv, and M. J. Kelly
Rapid Signaling of Estrogen in Hypothalamic Neurons Involves a Novel G-Protein-Coupled Estrogen Receptor that Activates Protein Kinase C
J. Neurosci.,
October 22, 2003;
23(29):
9529 - 9540.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Heger, M. Seney, E. Bless, G. A. Schwarting, M. Bilger, A. Mungenast, S. R. Ojeda, and S. A. Tobet
Overexpression of Glutamic Acid Decarboxylase-67 (GAD-67) in Gonadotropin-Releasing Hormone Neurons Disrupts Migratory Fate and Female Reproductive Function in Mice
Endocrinology,
June 1, 2003;
144(6):
2566 - 2579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bilger, S. Heger, D. W. Brann, A. Paredes, and S. R. Ojeda
A Conditional Tetracycline-Regulated Increase in Gamma Amino Butyric Acid Production near Luteinizing Hormone-Releasing Hormone Nerve Terminals Disrupts Estrous Cyclicity in the Rat
Endocrinology,
May 1, 2001;
142(5):
2102 - 2114.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Terasawa and D. L. Fernandez
Neurobiological Mechanisms of the Onset of Puberty in Primates
Endocr. Rev.,
February 1, 2001;
22(1):
111 - 151.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. E. Majdoubi, A. Sahu, and T. M. Plant
Changes in Hypothalamic Gene Expression Associated with the Arrest of Pulsatile Gonadotropin-Releasing Hormone Release during Infancy in the Agonadal Male Rhesus Monkey (Macaca mulatta)
Endocrinology,
September 1, 2000;
141(9):
3273 - 3277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Suter, C. R. Pohl, and M. E. Wilson
Circulating Concentrations of Nocturnal Leptin, Growth Hormone, and Insulin-Like Growth Factor-I Increase before the Onset of Puberty in Agonadal Male Monkeys: Potential Signals for the Initiation of Puberty
J. Clin. Endocrinol. Metab.,
February 1, 2000;
85(2):
808 - 814.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. E. Claypool, E. Kasuya, Y. Saitoh, F. Marzban, and E. Terasawa
N-Methyl D,L-Aspartate Induces the Release of Luteinizing Hormone-Releasing Hormone in the Prepubertal and Pubertal Female Rhesus Monkey as Measured by in Vivo Push-Pull Perfusion in the Stalk-Median Eminence
Endocrinology,
January 1, 2000;
141(1):
219 - 228.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. L. Keen, A. J. Burich, D. Mitsushima, E. Kasuya, and E. Terasawa
Effects of Pulsatile Infusion of the GABAA Receptor Blocker Bicuculline on the Onset of Puberty in Female Rhesus Monkeys
Endocrinology,
November 1, 1999;
140(11):
5257 - 5266.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Kasuya, C. L. Nyberg, K. Mogi, and E. Terasawa
A Role of {gamma}-Amino Butyric Acid (GABA) and Glutamate in Control of Puberty in Female Rhesus Monkeys: Effect of an Antisense Oligodeoxynucleotide for GAD67 Messenger Ribonucleic Acid and MK801 on Luteinizing Hormone-Releasing Hormone Release
Endocrinology,
February 1, 1999;
140(2):
705 - 712.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. E. Majdoubi, A. Sahu, S. Ramaswamy, and T. M. Plant
Neuropeptide Y: A hypothalamic brake restraining the onset of puberty in primates
PNAS,
May 23, 2000;
97(11):
6179 - 6184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
