WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Discover www.zeiss.de/functionality
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baird, D. H.
Right arrow Articles by Mason, C. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baird, D. H.
Right arrow Articles by Mason, C. A.
Right arrowPubmed/NCBI databases
*Compound via MeSH
*Substance via MeSH

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 16, 2642-2648, Copyright © 1996 by Society for Neuroscience

ARTICLE

Arrest of afferent axon extension by target neurons in vitro is regulated by the NMDA receptor

DH Baird, E Trenkner and CA Mason
Department of Neurobiology and Anatomy, Medical College of Pennsylvania and Hahnemann University, Philadelphia, 19129, USA.

Cerebellar granule neurons in vitro specifically arrest the extension of their appropriate presynaptic axons, mossy fibers. This "stop- growing signal" may be an essential step in the formation and specificity of synapses. Here, we have tested whether ionotropic glutamate receptors are involved in the stop-growing signal. When explants of basilar pontine nuclei, a mossy fiber source, were cultured on granule neurons, most pontine neurites terminated <200 microm from their explant of origin, a criterion for the stop-growing signal. In contrast, treatment with the NMDA antagonist D(-)-2-amino-5- phosphonopentanoic acid (D-AP5) greatly increased the number of pontine neurites extending beyond 300 microm, whereas treatment with NMDA reduced the number of pontine neurites extending beyond 200 microm. A non-NMDA agonist (AMPA) and antagonist (6-cyano-7-nitroquinoxaline-2,3- dione) did not alter pontine neurite lengths. None of these agents affected neurite outgrowth from pontine explants in the absence of granule neurons, nor did any agent affect the survival of granule neurons. These results indicate that NMDA and D-AP5 specifically perturb an interaction between axons and target cells necessary for the stop-growing signal, and that NMDA receptors are critical for the development of a major cerebellar afferent system. These findings also suggest that NMDA-sensitive refinement of axon arbors during later development may involve the direct regulation of axon extension by target neurons.


This article has been cited by other articles:


Home page
 J. Neurosci.Home page
S. B. Sann
Growth Cone Stop Signals: Inviting to Stay or Sending Away?
J. Neurosci., August 30, 2006; 26(35): 8879 - 8880.
[Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. C. Manzini, M. S. Ward, Q. Zhang, M. D. Lieberman, and C. A. Mason
The stop signal revised: immature cerebellar granule neurons in the external germinal layer arrest pontine mossy fiber growth.
J. Neurosci., May 31, 2006; 26(22): 6040 - 6051.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. Duch and R. B. Levine
Changes in Calcium Signaling During Postembryonic Dendritic Growth in Manduca sexta
J Neurophysiol, March 1, 2002; 87(3): 1415 - 1425.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. B. Belousov, B. F. O'Hara, and J. V. Denisova
Acetylcholine Becomes the Major Excitatory Neurotransmitter in the Hypothalamus In Vitro in the Absence of Glutamate Excitation
J. Neurosci., March 15, 2001; 21(6): 2015 - 2027.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. B. Gingrich, C. E. Junge, P. Lyuboslavsky, and S. F. Traynelis
Potentiation of NMDA Receptor Function by the Serine Protease Thrombin
J. Neurosci., June 15, 2000; 20(12): 4582 - 4595.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
D.-J. Zou and H. T. Cline
Postsynaptic Calcium/Calmodulin-Dependent Protein Kinase II Is Required to Limit Elaboration of Presynaptic and Postsynaptic Neuronal Arbors
J. Neurosci., October 15, 1999; 19(20): 8909 - 8918.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. A. Rabacchi, J. M. Solowska, B. Kruk, Y. Luo, J. A. Raper, and D. H. Baird
Collapsin-1/Semaphorin-III/D Is Regulated Developmentally in Purkinje Cells and Collapses Pontocerebellar Mossy Fiber Neuronal Growth Cones
J. Neurosci., June 1, 1999; 19(11): 4437 - 4448.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. L. Doughty, A. Lohof, F. Selimi, N. Delhaye-Bouchaud, and J. Mariani
Afferent-Target Cell Interactions in the Cerebellum: Negative Effect of Granule Cells on Purkinje Cell Development in Lurcher Mice
J. Neurosci., May 1, 1999; 19(9): 3448 - 3456.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. Masuko, K. Makino, H. Kuwahara, K. Fukunaga, T. Sudo, N. Araki, H. Yamamoto, Y. Yamada, E. Miyamoto, and H. Saya
Interaction of NE-dlg/SAP102, a Neuronal and Endocrine Tissue-specific Membrane-associated Guanylate Kinase Protein, with Calmodulin and PSD-95/SAP90. A POSSIBLE REGULATORY ROLE IN MOLECULAR CLUSTERING AT SYNAPTIC SITES
J. Biol. Chem., February 26, 1999; 274(9): 5782 - 5790.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S.-Y. Lin and M. Constantine-Paton
Suppression of Sprouting: An Early Function of NMDA Receptors in the Absence of AMPA/Kainate Receptor Activity
J. Neurosci., May 15, 1998; 18(10): 3725 - 3737.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Metzger, S. Wiese, and M. Sendtner
Effect of Glutamate on Dendritic Growth in Embryonic Rat Motoneurons
J. Neurosci., March 1, 1998; 18(5): 1735 - 1742.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-