 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 16, 2684-2692, Copyright © 1996 by Society for Neuroscience
Effects of chronic morphine administration on mu opioid receptor- stimulated [35S]GTPgammaS autoradiography in rat brain
LJ Sim, DE Selley, SI Dworkin and SR Childers
Department of Physiology and Pharmacology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina 27157, USA.
Chronic opiate administration results in the development of tolerance and
dependence, but the regulation of mu opioid receptor function during this
process is not clearly understood. To localize changes in mu opioid
receptor-coupled G-protein activity in various brain regions after chronic
morphine treatment, the present study examined mu opioid agonist-stimulated
[35S]GTPgammaS binding to brain sections by in vitro autoradiography. Rats
were treated for 12 d with increasing doses (10- 320mg . kg-1 . d-1) of
morphine. Control rats were injected with either saline or a single acute
injection of morphine (20 mg/kg). mu opioid- stimulated [35S]GTPgammaS
binding was measured by autoradiography of brain sections in the presence
and absence of the mu opioid-selective agonist DAMGO. In rats injected with
a single acute dose of morphine, no significant changes were detected in
basal or agonist-stimulated [35S]GTPgammaS binding in any region. In
sections from chronic morphine- treated rats, however, DAMGO-stimulated
[35S]GTPgammaS binding was reduced significantly compared with control rats
in the following brain- stem nuclei: dorsal raphe nucleus, locus coeruleus,
lateral and medial parabrachial nuclei, and commissural nucleus tractus
solitarius. No significant changes were observed in several other brain
regions, including the nucleus accumbens, amygdala, thalamus, and
substantia nigra. These data indicate that chronic morphine administration
results in reductions in mu opioid activation of G-proteins in specific
brainstem nuclei involved in physiological homeostasis and autonomic
function, which may have implications in the development of opiate
tolerance and physical dependence.
This article has been cited by other articles:
|
|
|
|
 
M. Shy, S. Chakrabarti, and A. R. Gintzler
Plasticity of Adenylyl Cyclase-Related Signaling Sequelae after Long-Term Morphine Treatment
Mol. Pharmacol.,
March 1, 2008;
73(3):
868 - 879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Chakrabarti and A. R. Gintzler
Phosphorylation of G{alpha}s Influences Its Association with the {micro}-Opioid Receptor and Is Modulated by Long-Term Morphine Exposure
Mol. Pharmacol.,
September 1, 2007;
72(3):
753 - 760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhou, J. Bendor, L. Hofmann, M. Randesi, A. Ho, and M. J. Kreek
Mu opioid receptor and orexin/hypocretin mRNA levels in the lateral hypothalamus and striatum are enhanced by morphine withdrawal.
J. Endocrinol.,
October 1, 2006;
191(1):
137 - 145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Georges, C. L. Moine, and G. Aston-Jones
No Effect of Morphine on Ventral Tegmental Dopamine Neurons during Withdrawal
J. Neurosci.,
May 24, 2006;
26(21):
5720 - 5726.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. K. Chelikani, A. C. Haver, J. R. Reeve Jr., D. A. Keire, and R. D. Reidelberger
Daily, intermittent intravenous infusion of peptide YY(3-36) reduces daily food intake and adiposity in rats
Am J Physiol Regulatory Integrative Comp Physiol,
February 1, 2006;
290(2):
R298 - R305.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. N. Talbot, H. K. Happe, and L. C. Murrin
{micro} Opioid Receptor Coupling to Gi/o Proteins Increases during Postnatal Development in Rat Brain
J. Pharmacol. Exp. Ther.,
August 1, 2005;
314(2):
596 - 602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. O'Connor, L. J. Porrino, H. M. L. Davies, and S. R. Childers
Time-Dependent Changes in Receptor/G-Protein Coupling in Rat Brain following Chronic Monoamine Transporter Blockade
J. Pharmacol. Exp. Ther.,
May 1, 2005;
313(2):
510 - 517.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Kreek, G. Bart, C. Lilly, K. S. Laforge, and D. A. Nielsen
Pharmacogenetics and Human Molecular Genetics of Opiate and Cocaine Addictions and Their Treatments
Pharmacol. Rev.,
March 1, 2005;
57(1):
1 - 26.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. Jutkiewicz, N. P. Walker, J. E. Folk, K. C. Rice, P. S. Portoghese, J. H. Woods, and J. R. Traynor
Comparison of Peptidic and Nonpeptidic {delta}-Opioid Agonists on Guanosine 5'-O-(3-[35S]thio)triphosphate ([35S]GTP{gamma}S) Binding in Brain Slices from Sprague-Dawley Rats
J. Pharmacol. Exp. Ther.,
March 1, 2005;
312(3):
1314 - 1320.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Bohn, L. A. Dykstra, R. J. Lefkowitz, M. G. Caron, and L. S. Barak
Relative Opioid Efficacy Is Determined by the Complements of the G Protein-Coupled Receptor Desensitization Machinery
Mol. Pharmacol.,
July 1, 2004;
66(1):
106 - 112.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Zachariou, D. Georgescu, N. Sanchez, Z. Rahman, R. DiLeone, O. Berton, R. L. Neve, L. J. Sim-Selley, D. E. Selley, S. J. Gold, et al.
Essential role for RGS9 in opiate action
PNAS,
November 11, 2003;
100(23):
13656 - 13661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Wilson, D. M. Nicklous, V. J. Aloyo, and K. J. Simansky
An orexigenic role for {micro}-opioid receptors in the lateral parabrachial nucleus
Am J Physiol Regulatory Integrative Comp Physiol,
November 1, 2003;
285(5):
R1055 - R1065.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Haberstock-Debic, M. Wein, M. Barrot, E. E. O. Colago, Z. Rahman, R. L. Neve, V. M. Pickel, E. J. Nestler, M. von Zastrow, and A. L. Svingos
Morphine Acutely Regulates Opioid Receptor Trafficking Selectively in Dendrites of Nucleus Accumbens Neurons
J. Neurosci.,
May 15, 2003;
23(10):
4324 - 4332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-X. Xi, S. Ramamoorthy, H. Shen, R. Lake, D. J. Samuvel, and P. W. Kalivas
GABA Transmission in the Nucleus Accumbens Is Altered after Withdrawal from Repeated Cocaine
J. Neurosci.,
April 15, 2003;
23(8):
3498 - 3505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Nitsche, A. G. P. Schuller, M. A. King, M. Zengh, G. W. Pasternak, and J. E. Pintar
Genetic Dissociation of Opiate Tolerance and Physical Dependence in delta -Opioid Receptor-1 and Preproenkephalin Knock-Out Mice
J. Neurosci.,
December 15, 2002;
22(24):
10906 - 10913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Boudarine, O. Yegorov, A. Sterling-Dubrovsky, L. A. Devi, and Y. Berman
Developmental Changes in Opioid Peptides and Their Receptors in Cpefat/Cpefat Mice Lacking Peptide Processing Enzyme Carboxypeptidase E
J. Pharmacol. Exp. Ther.,
December 1, 2002;
303(3):
1317 - 1324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Fabian, B. Bozo, M. Szikszay, G. Horvath, C. J. Coscia, and M. Szucs
Chronic Morphine-Induced Changes in {micro}-Opioid Receptors and G Proteins of Different Subcellular Loci in Rat Brain
J. Pharmacol. Exp. Ther.,
August 1, 2002;
302(2):
774 - 780.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Vogt, L. J. Sim-Selley, S. R. Childers, R. G. Wiley, and B. A. Vogt
Colocalization of {micro}-Opioid Receptors and Activated G-Proteins in Rat Cingulate Cortex
J. Pharmacol. Exp. Ther.,
December 1, 2001;
299(3):
840 - 848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Navarro, M. R. A. Carrera, W. Fratta, O. Valverde, G. Cossu, L. Fattore, J. A. Chowen, R. Gomez, I. del Arco, M. A. Villanua, et al.
Functional Interaction between Opioid and Cannabinoid Receptors in Drug Self-Administration
J. Neurosci.,
July 15, 2001;
21(14):
5344 - 5350.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. T. Williams, M. J. Christie, and O. Manzoni
Cellular and Synaptic Adaptations Mediating Opioid Dependence
Physiol Rev,
January 1, 2001;
81(1):
299 - 343.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J. Sim-Selley, D. E. Selley, L. J. Vogt, S. R. Childers, and T. J. Martin
Chronic Heroin Self-Administration Desensitizes {micro} Opioid Receptor-Activated G-Proteins in Specific Regions of Rat Brain
J. Neurosci.,
June 15, 2000;
20(12):
4555 - 4562.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. C. Dalman and K. L. O'Malley
kappa -Opioid Tolerance and Dependence in Cultures of Dopaminergic Midbrain Neurons
J. Neurosci.,
July 15, 1999;
19(14):
5750 - 5757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shen and S. A. Thayer
Delta 9-Tetrahydrocannabinol Acts as a Partial Agonist to Modulate Glutamatergic Synaptic Transmission between Rat Hippocampal Neurons in Culture
Mol. Pharmacol.,
January 1, 1999;
55(1):
8 - 13.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. L. Ingram, C. W. Vaughan, E. E. Bagley, M. Connor, and M. J. Christie
Enhanced Opioid Efficacy in Opioid Dependence Is Caused by an Altered Signal Transduction Pathway
J. Neurosci.,
December 15, 1998;
18(24):
10269 - 10276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kovoor, J. P. Celver, A. Wu, and C. Chavkin
Agonist Induced Homologous Desensitization of µ-Opioid Receptors Mediated by G Protein-Coupled Receptor Kinases Is Dependent on Agonist Efficacy
Mol. Pharmacol.,
October 1, 1998;
54(4):
704 - 711.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. L. Capece, H. A. Baghdoyan, and R. Lydic
Carbachol Stimulates [35S]Guanylyl 5'-(gamma -Thio)-Triphosphate Binding in Rapid Eye Movement Sleep-Related Brainstem Nuclei of Rat
J. Neurosci.,
May 15, 1998;
18(10):
3779 - 3785.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Selley, Q. Liu, and S. R. Childers
Signal Transduction Correlates of Mu Opioid Agonist Intrinsic Efficacy: Receptor-Stimulated [35S]GTPgamma S Binding in mMOR-CHO Cells and Rat Thalamus
J. Pharmacol. Exp. Ther.,
May 1, 1998;
285(2):
496 - 505.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. E. Keith, B. Anton, S. R. Murray, P. A. Zaki, P. C. Chu, D. V. Lissin, G. Monteillet-Agius, P. L. Stewart, C. J. Evans, and M. v. Zastrow
µ-Opioid Receptor Internalization: Opiate Drugs Have Differential Effects on a Conserved Endocytic Mechanism In Vitro and in the Mammalian Brain
Mol. Pharmacol.,
March 1, 1998;
53(3):
377 - 384.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. Yabaluri and F. Medzihradsky
Down-regulation of µ-Opioid Receptor by Full but Not Partial Agonists Is Independent of G Protein Coupling
Mol. Pharmacol.,
November 1, 1997;
52(5):
896 - 902.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. Kovoor, V. Nappey, B. L. Kieffer, and C. Chavkin
µ and delta Opioid Receptors Are Differentially Desensitized by the Coexpression of beta -Adrenergic Receptor Kinase 2 and beta -Arrestin 2 in Xenopus Oocytes
J. Biol. Chem.,
October 31, 1997;
272(44):
27605 - 27611.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Nestler and G. K. Aghajanian
Molecular and Cellular Basis of Addiction
Science,
October 3, 1997;
278(5335):
58 - 63.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. S. Breivogel, L. J. Sim, and S. R. Childers
Regional Differences in Cannabinoid Receptor/G-protein Coupling in Rat Brain
J. Pharmacol. Exp. Ther.,
September 1, 1997;
282(3):
1632 - 1642.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. O. Aley and J. D. Levine
Dissociation of Tolerance and Dependence for Opioid Peripheral Antinociception in Rats
J. Neurosci.,
May 15, 1997;
17(10):
3907 - 3912.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. E. Selley, L. J. Sim, R. Xiao, Q. Liu, and S. R. Childers
{micro}-Opioid Receptor-Stimulated Guanosine-5'-O-(gamma -thio)-triphosphate Binding in Rat Thalamus and Cultured Cell Lines: Signal Transduction Mechanisms Underlying Agonist Efficacy
Mol. Pharmacol.,
January 1, 1997;
51(1):
87 - 96.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. J. Sim, R. E. Hampson, S. A. Deadwyler, and S. R. Childers
Effects of Chronic Treatment with Delta 9-Tetrahydrocannabinol on Cannabinoid-Stimulated [35S]GTPgamma S Autoradiography in Rat Brain
J. Neurosci.,
December 15, 1996;
16(24):
8057 - 8066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
