 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 17, Number 1, Issue of January 1, 1997 pp. 107-116
Copyright ©1997 Society for NeuroscienceA Direct Comparison of the Single-Channel Properties of Synaptic and Extrasynaptic NMDA Receptors
Received July 8, 1996; revised Sept. 30, 1996; accepted Oct. 18, 1996.
Beverley A. Clark, Mark Farrant, and Stuart G. Cull-Candy Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom
The assumption that synaptic and extrasynaptic glutamate receptors are similar underpins many studies that have sought to relate the behavior of channels in excised patches to the macroscopic properties of the EPSC. We have examined this issue for NMDA receptors in cerebellar granule cells, the small size of which allows the opening of individual synaptic NMDA channels to be resolved directly. We have used whole-cell patch-clamp recordings to determine the conductance and open time of NMDA channels activated during the EPSC and used cell-attached and outside-out recordings to examine NMDA receptors in somatic membrane. Conductance and open time of synaptic channels were indistinguishable from those of extrasynaptic channels in cell-attached patches. However, the channel conductance in outside-out patches was 20% lower than in cell-attached recordings. This change was partially reduced by dantrolene and phalloidin, suggesting that it may involve depolymerization of actin following Ca2+ release from intracellular stores. Our results demonstrate that synaptic and extrasynaptic NMDA receptors have similar microscopic properties. However, NMDA channel conductance is reduced following the formation of an outside-out patch.
Key words: cerebellum; granule cell; patch clamp; NMDA; single channel; synaptic; extrasynaptic
This article has been cited by other articles:
N. Dehorter, C. Guigoni, C. Lopez, J. Hirsch, A. Eusebio, Y. Ben-Ari, and C. Hammond
Dopamine-Deprived Striatal GABAergic Interneurons Burst and Generate Repetitive Gigantic IPSCs in Medium Spiny Neurons
J. Neurosci., June 17, 2009; 29(24): 7776 - 7787.
[Abstract] [Full Text] [PDF]
A. Z. Harris and D. L. Pettit
Extrasynaptic and synaptic NMDA receptors form stable and uniform pools in rat hippocampal slices
J. Physiol., October 15, 2007; 584(2): 509 - 519.
[Abstract] [Full Text] [PDF]
D. A. DiGregorio, J. S. Rothman, T. A. Nielsen, and R. A. Silver
Desensitization Properties of AMPA Receptors at the Cerebellar Mossy Fiber Granule Cell Synapse
J. Neurosci., August 1, 2007; 27(31): 8344 - 8357.
[Abstract] [Full Text] [PDF]
C. Lu, Z. Fu, I. Karavanov, R. P. Yasuda, B. B. Wolfe, A. Buonanno, and S. Vicini
NMDA Receptor Subtypes at Autaptic Synapses of Cerebellar Granule Neurons
J Neurophysiol, November 1, 2006; 96(5): 2282 - 2294.
[Abstract] [Full Text] [PDF]
A. M. Binshtok, I. A. Fleidervish, R. Sprengel, and M. J. Gutnick
NMDA Receptors in Layer 4 Spiny Stellate Cells of the Mouse Barrel Cortex Contain the NR2C Subunit
J. Neurosci., January 11, 2006; 26(2): 708 - 715.
[Abstract] [Full Text] [PDF]
N. A. Lozovaya, S. E. Grebenyuk, T. Sh. Tsintsadze, B. Feng, D. T. Monaghan, and O. A. Krishtal
Extrasynaptic NR2B and NR2D subunits of NMDA receptors shape 'superslow' afterburst EPSC in rat hippocampus
J. Physiol., July 15, 2004; 558(2): 451 - 463.
[Abstract] [Full Text] [PDF]
E. D. Eggers and A. J. Berger
Mechanisms for the Modulation of Native Glycine Receptor Channels by Ethanol
J Neurophysiol, June 1, 2004; 91(6): 2685 - 2695.
[Abstract] [Full Text] [PDF]
N. O. Dalby and I. Mody
Activation of NMDA Receptors in Rat Dentate Gyrus Granule Cells by Spontaneous and Evoked Transmitter Release
J Neurophysiol, August 1, 2003; 90(2): 786 - 797.
[Abstract] [Full Text] [PDF]
C. I. O. Myme, K. Sugino, G. G. Turrigiano, and S. B. Nelson
The NMDA-to-AMPA Ratio at Synapses Onto Layer 2/3 Pyramidal Neurons Is Conserved Across Prefrontal and Visual Cortices
J Neurophysiol, August 1, 2003; 90(2): 771 - 779.
[Abstract] [Full Text] [PDF]
A. Momiyama, R A. Silver, M. Hausser, T. Notomi, Y. Wu, R. Shigemoto, and S. G Cull-Candy
The density of AMPA receptors activated by a transmitter quantum at the climbing fibre-Purkinje cell synapse in immature rats
J. Physiol., May 15, 2003; 549(1): 75 - 92.
[Abstract] [Full Text] [PDF]
G. Losi, K. Prybylowski, Z. Fu, J. Luo, R. J Wenthold, and S. Vicini
PSD-95 regulates NMDA receptors in developing cerebellar granule neurons of the rat
J. Physiol., April 1, 2003; 548(1): 21 - 29.
[Abstract] [Full Text] [PDF]
K. Satzler, L. F. Sohl, J. H. Bollmann, J. G. G. Borst, M. Frotscher, B. Sakmann, and J. H. R. Lubke
Three-Dimensional Reconstruction of a Calyx of Held and Its Postsynaptic Principal Neuron in the Medial Nucleus of the Trapezoid Body
J. Neurosci., December 15, 2002; 22(24): 10567 - 10579.
[Abstract] [Full Text] [PDF]
P. Rossi, E. Sola, V. Taglietti, T. Borchardt, F. Steigerwald, J. K. Utvik, O. P. Ottersen, G. Kohr, and E. D'Angelo
NMDA Receptor 2 (NR2) C-Terminal Control of NR Open Probability Regulates Synaptic Transmission and Plasticity at a Cerebellar Synapse
J. Neurosci., November 15, 2002; 22(22): 9687 - 9697.
[Abstract] [Full Text] [PDF]
K. Prybylowski, Z. Fu, G. Losi, L. M. Hawkins, J. Luo, K. Chang, R. J. Wenthold, and S. Vicini
Relationship between Availability of NMDA Receptor Subunits and Their Expression at the Synapse
J. Neurosci., October 15, 2002; 22(20): 8902 - 8910.
[Abstract] [Full Text] [PDF]
D. Billups, Y.-B. Liu, S. Birnstiel, and N. T. Slater
NMDA Receptor-Mediated Currents in Rat Cerebellar Granule and Unipolar Brush Cells
J Neurophysiol, April 1, 2002; 87(4): 1948 - 1959.
[Abstract] [Full Text] [PDF]
G. Abdrachmanova, J. Teisinger, and L. Vyklicky Jr
Axotomy-induced changes in the properties of NMDA receptor channels in rat spinal cord motoneurons
J. Physiol., January 1, 2002; 538(1): 53 - 63.
[Abstract] [Full Text] [PDF]
A. Frick, W. Zieglgansberger, and H.-U. Dodt
Glutamate Receptors Form Hot Spots on Apical Dendrites of Neocortical Pyramidal Neurons
J Neurophysiol, September 1, 2001; 86(3): 1412 - 1421.
[Abstract] [Full Text] [PDF]
Y. Li-Smerin, E. S Levitan, and J. W Johnson
Free intracellular Mg2+ concentration and inhibition of NMDA responses in cultured rat neurons
J. Physiol., June 15, 2001; 533(3): 729 - 743.
[Abstract] [Full Text] [PDF]
J. R. Cottrell, G. R. Dube, C. Egles, and G. Liu
Distribution, Density, and Clustering of Functional Glutamate Receptors Before and After Synaptogenesis in Hippocampal Neurons
J Neurophysiol, September 1, 2000; 84(3): 1573 - 1587.
[Abstract] [Full Text] [PDF]
L. Cathala, C. Misra, and S. Cull-Candy
Developmental Profile of the Changing Properties of NMDA Receptors at Cerebellar Mossy Fiber-Granule Cell Synapses
J. Neurosci., August 15, 2000; 20(16): 5899 - 5905.
[Abstract] [Full Text] [PDF]
A. G. Carter and W. G. Regehr
Prolonged Synaptic Currents and Glutamate Spillover at the Parallel Fiber to Stellate Cell Synapse
J. Neurosci., June 15, 2000; 20(12): 4423 - 4434.
[Abstract] [Full Text] [PDF]
C. Misra, S. G Brickley, D. J A Wyllie, and S. G Cull-Candy
Slow deactivation kinetics of NMDA receptors containing NR1 and NR2D subunits in rat cerebellar Purkinje cells
J. Physiol., June 1, 2000; 525(2): 299 - 305.
[Abstract] [Full Text] [PDF]
A. Momiyama
Distinct synaptic and extrasynaptic NMDA receptors identified in dorsal horn neurones of the adult rat spinal cord
J. Physiol., March 15, 2000; 523(3): 621 - 628.
[Abstract] [Full Text] [PDF]
Y. Li-Smerin, E. Aizenman, and J. W. Johnson
Inhibition by Intracellular Mg2+ of Recombinant N-Methyl-D-aspartate Receptors Expressed in Chinese Hamster Ovary Cells
J. Pharmacol. Exp. Ther., March 1, 2000; 292(3): 1104 - 1110.
[Abstract] [Full Text]
R. Sattler, Z. Xiong, W.-Y. Lu, J. F. MacDonald, and M. Tymianski
Distinct Roles of Synaptic and Extrasynaptic NMDA Receptors in Excitotoxicity
J. Neurosci., January 1, 2000; 20(1): 22 - 33.
[Abstract] [Full Text] [PDF]
G. Rumbaugh and S. Vicini
Distinct Synaptic and Extrasynaptic NMDA Receptors in Developing Cerebellar Granule Neurons
J. Neurosci., December 15, 1999; 19(24): 10603 - 10610.
[Abstract] [Full Text] [PDF]
D. N Lieberman and I. Mody
Properties of single NMDA receptor channels in human dentate gyrus granule cells
J. Physiol., July 1, 1999; 518(1): 55 - 70.
[Abstract] [Full Text] [PDF]
S. G. Brickley, S. G. Cull-Candy, and M. Farrant
Single-Channel Properties of Synaptic and Extrasynaptic GABAA Receptors Suggest Differential Targeting of Receptor Subtypes
J. Neurosci., April 15, 1999; 19(8): 2960 - 2973.
[Abstract] [Full Text] [PDF]
E. S. Levine, R. A. Crozier, I. B. Black, and M. R. Plummer
Brain-derived neurotrophic factor modulates hippocampal synaptic transmission by increasing N-methyl-D-aspartic acid receptor activity
PNAS, August 18, 1998; 95(17): 10235 - 10239.
[Abstract] [Full Text] [PDF]
S. Sequeira and J. Nasstrom
Low-Affinity Kainate Receptors and Long-Lasting Depression of NMDA-Receptor-Mediated Currents in Rat Superficial Dorsal Horn
J Neurophysiol, August 1, 1998; 80(2): 895 - 902.
[Abstract] [Full Text] [PDF]
T. A. Cleland and A. I. Selverston
Inhibitory Glutamate Receptor Channels in Cultured Lobster Stomatogastric Neurons
J Neurophysiol, June 1, 1998; 79(6): 3189 - 3196.
[Abstract] [Full Text] [PDF]
G. Stocca and S. Vicini
Increased contribution of NR2A subunit to synaptic NMDA receptors in developing rat cortical neurons
J. Physiol., February 15, 1998; 507(1): 13 - 24.
[Abstract] [Full Text] [PDF]
E. Palma, L. Maggi, F. Eusebi, and R. Miledi
Neuronal nicotinic threonine-for-leucine 247 alpha 7 mutant receptors show different gating kinetics when activated by acetylcholine or by the noncompetitive agonist 5-hydroxytryptamine
PNAS, September 2, 1997; 94(18): 9915 - 9919.
[Abstract] [Full Text] [PDF]
G. Abdrachmanova, J. Teisinger, and L. Vyklicy Jr
AXOTOMY-INDUCED CHANGES IN THE PROPERTIES OF NMDA RECEPTOR CHANNELS IN RAT SPINAL CORD MOTONEURONS
J. Physiol., December 3, 2001; (2001) 200101279.
[Abstract] [PDF]
|
|
|
|
 |
|