WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Bioscience Autoneuron
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (247)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by D'Arcangelo, G.
Right arrow Articles by Curran, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D'Arcangelo, G.
Right arrow Articles by Curran, T.

 Previous Article  |  Next Article 

Volume 17, Number 1, Issue of January 1, 1997 pp. 23-31
Copyright ©1997 Society for Neuroscience

Reelin Is a Secreted Glycoprotein Recognized by the CR-50 Monoclonal Antibody

Received Sept. 5, 1996; revised Oct. 3, 1996; accepted Oct. 4, 1996.

Gabriella D'Arcangelo1, Kazunori Nakajima1, 2, Takaki Miyata2, 3, Masaharu Ogawa3, Katsuhiko Mikoshiba2, 4, and Tom Curran1

1 Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, 2 Molecular Neurobiology Laboratory, Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN), Tsukuba, Ibaraki 305, Japan, 3 Department of Physiology, Kochi Medical School, Nankoku, Kochi 783, Japan, and 4 Department of Molecular Neurobiology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108, Japan

The neurological mouse mutant strain reeler displays abnormal laminar organization of several brain structures as a consequence of a defect in cell migration during neurodevelopment. This phenotype is a result of the disruption of reelin, a gene encoding a protein that has several structural characteristics of extracellular matrix proteins. To understand the molecular basis of the action of Reelin on neuronal migration, we constructed a full-length reelin clone and used it to direct Reelin expression. Here, we demonstrate that Reelin is a secreted glycoprotein and that a highly charged C-terminal region is essential for secretion. In addition, we demonstrate that an amino acid sequence present in the N-terminal region of Reelin contains an epitope that is recognized by the CR-50 monoclonal antibody. CR-50 was raised against an antigen expressed in normal mouse brain that is absent in reeler mice. The interaction of CR-50 with its epitope leads to the disruption of neural cell aggregation in vitro. Here, we used CR-50 to precipitate Reelin from reticulocyte extracts programmed with reelin mRNA, from cells transfected with reelin clones, and from cerebellar explants. The reelin gene product seems to function as an instructive signal in the regulation of neuronal migration.

Key words: reeler; cerebral cortex; cerebellum; extracellular matrix; glycosylation; mutant mice; neuronal migration




This article has been cited by other articles:


Home page
 DevelopmentHome page
A. K. Voss, J. M. Britto, M. P. Dixon, B. N. Sheikh, C. Collin, S.-S. Tan, and T. Thomas
C3G regulates cortical neuron migration, preplate splitting and radial glial cell attachment
Development, June 15, 2008; 135(12): 2139 - 2149.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
N. Ichinohe, A. Knight, M. Ogawa, T. Ohshima, K. Mikoshiba, Y. Yoshihara, T. Terashima, and K. S. Rockland
Unusual Patch-Matrix Organization in the Retrosplenial Cortex of the reeler Mouse and Shaking Rat Kawasaki
Cereb Cortex, May 1, 2008; 18(5): 1125 - 1138.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
N. Mimura, S. Yuasa, M. Soma, H. Jin, K. Kimura, S. Goto, H. Koseki, and T. Aoe
Altered Quality Control in the Endoplasmic Reticulum Causes Cortical Dysplasia in Knock-In Mice Expressing a Mutant BiP
Mol. Cell. Biol., January 1, 2008; 28(1): 293 - 301.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
I. Hack, S. Hellwig, D. Junghans, B. Brunne, H. H. Bock, S. Zhao, and M. Frotscher
Divergent roles of ApoER2 and Vldlr in the migration of cortical neurons
Development, November 1, 2007; 134(21): 3883 - 3891.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Jossin and A. M. Goffinet
Reelin Signals through Phosphatidylinositol 3-Kinase and Akt To Control Cortical Development and through mTor To Regulate Dendritic Growth
Mol. Cell. Biol., October 15, 2007; 27(20): 7113 - 7124.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. Achilles, A. Okabe, M. Ikeda, C. Shimizu-Okabe, J. Yamada, A. Fukuda, H. J. Luhmann, and W. Kilb
Kinetic Properties of Cl Uptake Mediated by Na+-Dependent K+-2Cl Cotransport in Immature Rat Neocortical Neurons
J. Neurosci., August 8, 2007; 27(32): 8616 - 8627.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. Nakano, T. Kohno, T. Hibi, S. Kohno, A. Baba, K. Mikoshiba, K. Nakajima, and M. Hattori
The Extremely Conserved C-terminal Region of Reelin Is Not Necessary for Secretion but Is Required for Efficient Activation of Downstream Signaling
J. Biol. Chem., July 13, 2007; 282(28): 20544 - 20552.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Yasui, T. Nogi, T. Kitao, Y. Nakano, M. Hattori, and J. Takagi
Structure of a receptor-binding fragment of reelin and mutational analysis reveal a recognition mechanism similar to endocytic receptors
PNAS, June 12, 2007; 104(24): 9988 - 9993.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. V. Nielsen, F. H. Nielsen, R. Ismail, J. Noraberg, and N. A. Jensen
Hippocampus-like corticoneurogenesis induced by two isoforms of the BTB-zinc finger gene Zbtb20 in mice
Development, March 15, 2007; 134(6): 1133 - 1140.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Qiu and E. J. Weeber
Reelin Signaling Facilitates Maturation of CA1 Glutamatergic Synapses
J Neurophysiol, March 1, 2007; 97(3): 2312 - 2321.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
S. Simo, L. Pujadas, M. F. Segura, A. L. Torre, J. A. Del Rio, J. M. Urena, J. X. Comella, and E. Soriano
Reelin Induces the Detachment of Postnatal Subventricular Zone Cells and the Expression of the Egr-1 through Erk1/2 Activation
Cereb Cortex, February 1, 2007; 17(2): 294 - 303.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Yamashita, Y. Uchida, T. Ohshima, S.-i. Hirai, F. Nakamura, M. Taniguchi, K. Mikoshiba, J. Honnorat, P. Kolattukudy, N. Thomasset, et al.
Collapsin Response Mediator Protein 1 Mediates Reelin Signaling in Cortical Neuronal Migration
J. Neurosci., December 20, 2006; 26(51): 13357 - 13362.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Honda and K. Nakajima
Mouse Disabled1 (DAB1) Is a Nucleocytoplasmic Shuttling Protein
J. Biol. Chem., December 15, 2006; 281(50): 38951 - 38965.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Qiu, L. F. Zhao, K. M. Korwek, and E. J. Weeber
Differential Reelin-Induced Enhancement of NMDA and AMPA Receptor Activity in the Adult Hippocampus
J. Neurosci., December 13, 2006; 26(50): 12943 - 12955.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. Pla, V. Borrell, N. Flames, and O. Marin
Layer acquisition by cortical GABAergic interneurons is independent of Reelin signaling.
J. Neurosci., June 28, 2006; 26(26): 6924 - 6934.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Mayer, S. Duit, C. Hauser, W. J. Schneider, and J. Nimpf
Reconstitution of the Reelin Signaling Pathway in Fibroblasts Demonstrates that Dab1 Phosphorylation Is Independent of Receptor Localization in Lipid Rafts
Mol. Cell. Biol., January 1, 2006; 26(1): 19 - 27.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Cariboni, S. Rakic, A. Liapi, R. Maggi, A. Goffinet, and J. G. Parnavelas
Reelin provides an inhibitory signal in the migration of gonadotropin-releasing hormone neurons
Development, November 1, 2005; 132(21): 4709 - 4718.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. Sinagra, D. Verrier, D. Frankova, K. M. Korwek, J. Blahos, E. J. Weeber, O. J. Manzoni, and P. Chavis
Reelin, Very-Low-Density Lipoprotein Receptor, and Apolipoprotein E Receptor 2 Control Somatic NMDA Receptor Composition during Hippocampal Maturation In Vitro
J. Neurosci., June 29, 2005; 25(26): 6127 - 6136.
[Abstract] [Full Text] [PDF]

Home page
 J Child NeurolHome page
S. Kanatani, H. Tabata, and K. Nakajima
Topical Review: Neuronal Migration in Cortical Development
J Child Neurol, April 1, 2005; 20(4): 274 - 279.
[Abstract] [PDF]

Home page
 J Child NeurolHome page
M. F. McManus and J. A. Golden
Topical Review: Neuronal Migration in Developmental Disorders
J Child Neurol, April 1, 2005; 20(4): 280 - 286.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
P. C. Stolt, Y. Chen, P. Liu, H. H. Bock, S. C. Blacklow, and J. Herz
Phosphoinositide Binding by the Disabled-1 PTB Domain Is Necessary for Membrane Localization and Reelin Signal Transduction
J. Biol. Chem., March 11, 2005; 280(10): 9671 - 9677.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
S. Zhao, X. Chai, E. Forster, and M. Frotscher
Reelin is a positional signal for the lamination of dentate granule cells
Development, October 15, 2004; 131(20): 5117 - 5125.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
H. Abraham, C. G. Perez-Garcia, and G. Meyer
p73 and Reelin in Cajal-Retzius Cells of the Developing Human Hippocampal Formation
Cereb Cortex, May 1, 2004; 14(5): 484 - 495.
[Abstract] [Full Text] [PDF]

Home page
 J Child NeurolHome page
S. Kanatani, H. Tabata, and K. Nakajima
Topical Review: Neuronal Migration in Cortical Development
J Child Neurol, March 1, 2004; 19(3): 274 - 279.
[Abstract] [PDF]

Home page
 J Child NeurolHome page
M. F. McManus and J. A. Golden
Topical Review: Neuronal Migration in Developmental Disorders
J Child Neurol, March 1, 2004; 19(3): 280 - 286.
[Abstract] [PDF]

Home page
 J. Neurosci.Home page
Y. Jossin, N. Ignatova, T. Hiesberger, J. Herz, C. Lambert de Rouvroit, and A. M. Goffinet
The Central Fragment of Reelin, Generated by Proteolytic Processing In Vivo, Is Critical to Its Function during Cortical Plate Development
J. Neurosci., January 14, 2004; 24(2): 514 - 521.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Y. Jossin, M. Ogawa, C. Metin, F. Tissir, and A. M. Goffinet
Inhibition of Src Family Kinases and Non-Classical Protein Kinases C Induce a Reeler-Like Malformation of Cortical Plate Development
J. Neurosci., October 29, 2003; 23(30): 9953 - 9959.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A. Pramatarova, P. G. Ochalski, K. Chen, A. Gropman, S. Myers, K.-T. Min, and B. W. Howell
Nck{beta} Interacts with Tyrosine-Phosphorylated Disabled 1 and Redistributes in Reelin-Stimulated Neurons
Mol. Cell. Biol., October 15, 2003; 23(20): 7210 - 7221.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Morante-Oria, A. Carleton, B. Ortino, E. J. Kremer, A. Fairen, and P.-M. Lledo
Subpallial origin of a population of projecting pioneer neurons during corticogenesis
PNAS, October 14, 2003; 100(21): 12468 - 12473.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
S. Rakic and N. Zecevic
Emerging Complexity of Layer I in Human Cerebral Cortex
Cereb Cortex, October 1, 2003; 13(10): 1072 - 1083.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. Hartfuss, E. Forster, H. H. Bock, M. A. Hack, P. Leprince, J. M. Luque, J. Herz, M. Frotscher, and M. Gotz
Reelin signaling directly affects radial glia morphology and biochemical maturation
Development, October 1, 2003; 130(19): 4597 - 4609.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. C. Quattrocchi, C. Huang, S. Niu, M. Sheldon, D. Benhayon, J. Cartwright Jr., D. R. Mosier, F. Keller, and G. D'Arcangelo
Reelin Promotes Peripheral Synapse Elimination and Maturation
Science, August 1, 2003; 301(5633): 649 - 653.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Frotscher, C. A. Haas, and E. Forster
Reelin Controls Granule Cell Migration in the Dentate Gyrus by Acting on the Radial Glial Scaffold
Cereb Cortex, June 1, 2003; 13(6): 634 - 640.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
U. Beffert, G. Morfini, H. H. Bock, H. Reyna, S. T. Brady, and J. Herz
Reelin-mediated Signaling Locally Regulates Protein Kinase B/Akt and Glycogen Synthase Kinase 3beta
J. Biol. Chem., December 13, 2002; 277(51): 49958 - 49964.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
V. Martinez-Cerdeno, M. J. Galazo, C. Cavada, and F. Clasca
Reelin Immunoreactivity in the Adult Primate Brain: Intracellular Localization in Projecting and Local Circuit Neurons of the Cerebral Cortex, Hippocampus and Subcortical Regions
Cereb Cortex, December 1, 2002; 12(12): 1298 - 1311.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
S. Nishikawa, S. Goto, T. Hamasaki, K. Yamada, and Y. Ushio
Involvement of Reelin and Cajal-Retzius Cells in the Developmental Formation of Vertical Columnar Structures in the Cerebral Cortex: Evidence from the Study of Mouse Presubicular Cortex
Cereb Cortex, October 1, 2002; 12(10): 1024 - 1030.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Forster, A. Tielsch, B. Saum, K. H. Weiss, C. Johanssen, D. Graus-Porta, U. Muller, and M. Frotscher
Reelin, Disabled 1, and beta 1 integrins are required for the formation of the radial glial scaffold in the hippocampus
PNAS, October 1, 2002; 99(20): 13178 - 13183.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. A. Haas, O. Dudeck, M. Kirsch, C. Huszka, G. Kann, S. Pollak, J. Zentner, and M. Frotscher
Role for Reelin in the Development of Granule Cell Dispersion in Temporal Lobe Epilepsy
J. Neurosci., July 15, 2002; 22(14): 5797 - 5802.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Sugitani, S. Nakai, O. Minowa, M. Nishi, K.-i. Jishage, H. Kawano, K. Mori, M. Ogawa, and T. Noda
Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons
Genes & Dev., July 15, 2002; 16(14): 1760 - 1765.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Meyer, C. G. Perez-Garcia, H. Abraham, and D. Caput
Expression of p73 and Reelin in the Developing Human Cortex
J. Neurosci., June 15, 2002; 22(12): 4973 - 4986.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. M. Kim, T. Qu, V. Kriho, P. Lacor, N. Smalheiser, G. D. Pappas, A. Guidotti, E. Costa, and K. Sugaya
Reelin function in neural stem cell biology
PNAS, March 19, 2002; 99(6): 4020 - 4025.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. C. Quattrocchi, F. Wannenes, A. M. Persico, S. A. Ciafre, G. D'Arcangelo, M. G. Farace, and F. Keller
Reelin Is a Serine Protease of the Extracellular Matrix
J. Biol. Chem., January 4, 2002; 277(1): 303 - 309.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
T. M. Herrick and J. A. Cooper
A hypomorphic allele of dab1 reveals regional differences in reelin-Dab1 signaling during brain development
Development, January 2, 2002; 129(3): 787 - 796.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
A. J. Barkovich, R. I. Kuzniecky, G. D. Jackson, R. Guerrini, and W. B. Dobyns
Classification system for malformations of cortical development: Update 2001
Neurology, December 26, 2001; 57(12): 2168 - 2178.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
E. C. Gilmore and K. Herrup
Neocortical Cell Migration: GABAergic Neurons and Cells in Layers I and VI Move in a Cyclin-Dependent Kinase 5-Independent Manner
J. Neurosci., December 15, 2001; 21(24): 9690 - 9700.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
V. Hammond, B. Howell, L. Godinho, and S.-S. Tan
disabled-1 Functions Cell Autonomously during Radial Migration and Cortical Layering of Pyramidal Neurons
J. Neurosci., November 15, 2001; 21(22): 8798 - 8808.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S.-M. Lu, N. Zecevic, and H. H. Yeh
Distinct NMDA and AMPA Receptor-Mediated Responses in Mouse and Human Cajal-Retzius Cells
J Neurophysiol, November 1, 2001; 86(5): 2642 - 2646.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
N. Zecevic and P. Rakic
Development of Layer I Neurons in the Primate Cerebral Cortex
J. Neurosci., August 1, 2001; 21(15): 5607 - 5619.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
E. Forster, S. Zhao, and M. Frotscher
Hyaluronan-associated adhesive cues control fiber segregation in the hippocampus
Development, August 1, 2001; 128(15): 3029 - 3039.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
T. Hamasaki, S. Goto, S. Nishikawa, and Y. Ushio
Early-generated Preplate Neurons in the Developing Telencephalon: Inward Migration into the Developing Striatum
Cereb Cortex, May 1, 2001; 11(5): 474 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
A. Wynshaw-Boris and M. J. Gambello
LIS1 and dynein motor function in neuronal migration and development
Genes & Dev., March 15, 2001; 15(6): 639 - 651.
[Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Ohshima, M. Ogawa, Veeranna, M. Hirasawa, G. Longenecker, K. Ishiguro, H. C. Pant, R. O. Brady, A. B. Kulkarni, and K. Mikoshiba
Synergistic contributions of cyclin-dependant kinase 5/p35 and Reelin/Dab1 to the positioning of cortical neurons in the developing mouse brain
PNAS, February 8, 2001; (2001) 51628498.
[Abstract] [Full Text]

Home page
 Hum Mol GenetHome page
J. Sasaki, K. Ishikawa, K. Kobayashi, E. Kondo-Iida, M. Fukayama, H. Mizusawa, S. Takashima, Y. Sakakihara, Y. Nakamura, and T. Toda
Neuronal expression of the fukutin gene
Hum. Mol. Genet., December 1, 2000; 9(20): 3083 - 3090.
[Abstract] [Full Text] [PDF]

Home page
 Cereb CortexHome page
M. Frotscher, A. Drakew, and B. Heimrich
Role of Afferent Innervation and Neuronal Activity in Dendritic Development and Spine Maturation of Fascia Dentata Granule Cells
Cereb Cortex, October 1, 2000; 10(10): 946 - 951.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Utsunomiya-Tate, K.-i. Kubo, S.-i. Tate, M. Kainosho, E. Katayama, K. Nakajima, and K. Mikoshiba
Reelin molecules assemble together to form a large protein complex, which is inhibited by the function-blocking CR-50 antibody
PNAS, July 30, 2000; (2000) 160272497.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. W. Yip, Y. P. L. Yip, K. Nakajima, and C. Capriotti
Reelin controls position of autonomic neurons in the spinal cord
PNAS, June 30, 2000; (2000) 150040497.
[Abstract] [Full Text]

Home page
 Cereb CortexHome page
H. Super, J. A. Del Rio, A. Martinez, P. Perez-Sust, and E. Soriano
Disruption of Neuronal Migration and Radial Glia in the Developing Cerebral Cortex Following Ablation of Cajal-Retzius Cells
Cereb Cortex, June 1, 2000; 10(6): 602 - 613.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
G. Meyer, J. P. Schaaps, L. Moreau, and A. M. Goffinet
Embryonic and Early Fetal Development of the Human Neocortex
J. Neurosci., March 1, 2000; 20(5): 1858 - 1868.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. R. Smalheiser, E. Costa, A. Guidotti, F. Impagnatiello, J. Auta, P. Lacor, V. Kriho, and G. D. Pappas
Expression of reelin in adult mammalian blood, liver, pituitary pars intermedia, and adrenal chromaffin cells
PNAS, February 1, 2000; 97(3): 1281 - 1286.
[Abstract] [Full Text] [PDF]