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Volume 17, Number 1,
Issue of January 1, 1997
pp. 32-44
Copyright ©1997 Society for Neuroscience
A Novel Subunit for Shal K+ Channels Radically Alters
Activation and Inactivation
Received July 11, 1996; revised Sept. 17, 1996; accepted Oct. 7, 1996.
Timothy Jegla and
Lawrence Salkoff
Department of Anatomy and Neurobiology, Washington University
School of Medicine, St. Louis, Missouri 63110
Shal (Kv4) potassium channel genes encode classical subthreshold
A-currents, and their regulation may be a key factor in determining neuronal firing frequency. The inactivation rate of Shal channels is
increased by a presently unidentified class of proteins in both
Drosophila and mammals. We have cloned a novel Shal
channel subunit (jShal 1) from the jellyfish Polyorchis
penicillatus that alters Shal currents from both invertebrates
and vertebrates. When co-expressed with the conserved jellyfish Shal
homolog jShal1, jShal1 dramatically changes both the rate of
inactivation and voltage range of activation and steady-state
inactivation. jShal1 provides fast inactivation by a classic N-type
mechanism, which is independent of its effects on voltage dependence.
jShal1 forms functional channels only as a heteromultimer, and
jShal1 + jShal1 heteromultimers are functional only in a 2:2 subunit
stoichiometry.
Key words:
K+ channel;
Shal;
inactivation;
Polyorchis;
a-current;
heteromultimer;
jellyfish;
diploblast;
-subunit
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