WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (63)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Williams, J. A.
Right arrow Articles by Reiner, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Williams, J. A.
Right arrow Articles by Reiner, P. B.

 Previous Article  |  Next Article 

Volume 17, Number 1, Issue of January 1, 1997 pp. 420-427
Copyright ©1997 Society for Neuroscience

Nitric Oxide Production in Rat Thalamus Changes with Behavioral State, Local Depolarization, and Brainstem Stimulation

Received July 24, 1996; revised Oct. 3, 1996; accepted Oct. 9, 1996.

Julie A. Williams, Steven R. Vincent, and Peter B. Reiner

Kinsmen Laboratory of Neurological Research, Department of Psychiatry, Graduate Program in Neuroscience, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

Since its discovery as a putative neurotransmitter in the CNS, several functional roles have been suggested for nitric oxide (NO). However, few studies have investigated the role of NO in natural physiology. Because NO synthase (NOS) has been localized in regions believed to be important for attention and arousal, we hypothesized that NO production would be state-dependent. To test this hypothesis, we used in vivo microdialysis, coupled with the hemoglobin-trapping technique, to monitor extracellular NO concentrations in rat thalamus during wake, slow-wave sleep (SWS), and rapid eye movement (REM) sleep. The thalamus is known to receive a massive innervation from the NOS/cholinergic neurons in the mesopontine brainstem, which have been suggested to play a key role in EEG desynchronized states. To test whether thalamic NO output was sensitive to neuronal-dependent changes in the mesopontine brainstem, we measured thalamic NO concentration in response to electrical stimulation in the laterodorsal tegmentum (LDT) of anesthetized rats. Finally, the calcium dependence of NO release was tested by local depolarization with a high potassium dialysate or by addition of a calcium chelator. The results showed that (1) extracellular NO concentrations in the thalamus were high during wake and REM sleep and significantly lower during SWS, (2) thalamic NO release increased in response to LDT stimulation in both a site-specific and tetrodotoxin (TTX)-dependent manner, and (3) NO production was calcium-dependent. These data suggest that thalamic NO production may play a role in arousal.

Key words: nitric oxide; sleep; arousal; microdialysis; mesopontine cholinergic neurons; EEG




This article has been cited by other articles:


Home page
 J. Physiol.Home page
S. Yang and C. L. Cox
Excitatory and anti-oscillatory actions of nitric oxide in thalamus
J. Physiol., August 1, 2008; 586(15): 3617 - 3628.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
S. Yang and C. L. Cox
Modulation of Inhibitory Activity by Nitric Oxide in the Thalamus
J Neurophysiol, May 1, 2007; 97(5): 3386 - 3395.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
V. M. Pulgar, J. Zhang, G. A. Massmann, and J. P. Figueroa
Prolonged Mild Hypoxia Alters Fetal Sheep Electrocorticogram Activity
Reproductive Sciences, September 1, 2006; 13(6): 404 - 411.
[Abstract] [PDF]

Home page
 J. Neurophysiol.Home page
H. Huang, P. Ghosh, and A. N. van den Pol
Prefrontal Cortex-Projecting Glutamatergic Thalamic Paraventricular Nucleus-Excited by Hypocretin: A Feedforward Circuit That May Enhance Cognitive Arousal
J Neurophysiol, March 1, 2006; 95(3): 1656 - 1668.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. C. Ribeiro and L. Kapas
Day- and nighttime injection of a nitric oxide synthase inhibitor elicits opposite sleep responses in rats
Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2005; 289(2): R521 - R531.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
K. A. Kohlmeier, T. Inoue, and C. S. Leonard
Hypocretin/Orexin Peptide Signaling in the Ascending Arousal System: Elevation of Intracellular Calcium in the Mouse Dorsal Raphe and Laterodorsal Tegmentum
J Neurophysiol, July 1, 2004; 92(1): 221 - 235.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. G. Andreeva, P. Dikkes, P. M. Epstein, and P. A. Rosenberg
Expression of cGMP-Specific Phosphodiesterase 9A mRNA in the Rat Brain
J. Neurosci., November 15, 2001; 21(22): 9068 - 9076.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. S. Leonard, E. K. Michaelis, and K. M. Mitchell
Activity-Dependent Nitric Oxide Concentration Dynamics in the Laterodorsal Tegmental Nucleus In Vitro
J Neurophysiol, November 1, 2001; 86(5): 2159 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
C. S. Leonard, S. R. Rao, and T. Inoue
Serotonergic Inhibition of Action Potential Evoked Calcium Transients in NOS-Containing Mesopontine Cholinergic Neurons
J Neurophysiol, September 1, 2000; 84(3): 1558 - 1572.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
P. A. Rosenberg, Y. Li, M. Le, and Y. Zhang
Nitric Oxide-Stimulated Increase in Extracellular Adenosine Accumulation in Rat Forebrain Neurons in Culture Is Associated with ATP Hydrolysis and Inhibition of Adenosine Kinase Activity
J. Neurosci., August 15, 2000; 20(16): 6294 - 6301.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
I. Pose, S. Sampogna, M. H. Chase, and F. R. Morales
Cuneiform Neurons Activated during Cholinergically Induced Active Sleep in the Cat
J. Neurosci., May 1, 2000; 20(9): 3319 - 3327.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
A. C. Ribeiro, J. G. Gilligan, and L. Kapas
Systemic injection of a nitric oxide synthase inhibitor suppresses sleep responses to sleep deprivation in rats
Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2000; 278(4): R1048 - R1056.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. M. Uribe, S. Lee, and C. Rivier
Endotoxin Stimulates Nitric Oxide Production in the Paraventricular Nucleus of the Hypothalamus through Nitric Oxide Synthase I: Correlation with Hypothalamic-Pituitary-Adrenal Axis Activation
Endocrinology, December 1, 1999; 140(12): 5971 - 5981.
[Abstract] [Full Text]


-
-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2009 by Society for Neuroscience ONLINE ISSN: 1529-2401
-