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Volume 17, Number 1, Issue of January 1, 1997 pp. 58-69
Copyright ©1997 Society for NeuroscienceSingle-Channel Properties of Recombinant AMPA Receptors Depend on RNA Editing, Splice Variation, and Subunit Composition
Received July 8, 1996; revised Oct. 4, 1996; accepted Oct. 8, 1996.
Geoffrey T. Swanson1, 2, , Sunjeev K. Kamboj1, , and Stuart G. Cull-Candy1 1 Department of Pharmacology, University College London, London WC1E 6BT, United Kingdom, and 2 Molecular Neurobiology Laboratory, The Salk Institute, La Jolla, California 92037
Non-NMDA glutamate receptor subunits of the AMPA-preferring subfamily combine to form ion channels with heterogeneous functional properties. We have investigated the effects of RNA editing at the Q/R site, splice variation of the "flip/flop" cassette, and multimeric subunit assembly on the single-channel conductance and kinetic properties of the recombinant AMPA receptors formed from GluR2 and GluR4 expressed in HEK 293 cells. We found that AMPA receptor single-channel conductance was dependent on the Q/R site editing state of the subunits comprising the channel. Calcium-permeable (unedited) channels had resolvable single-channel events with main conductance states of 7-8 pS, whereas fully edited GluR2 channels had very low conductances of ~300 fS (estimated from noise analysis). Additionally, the flip splice variant of GluR4 conferred agonist-dependent conductance properties reminiscent of those found for a subset of AMPA receptors in cultured cerebellar granule cells. These results provide a description of the single-channel properties of certain recombinant AMPA receptors and suggest that the single-channel conductance may be determined by the expression of edited GluR2 subunits in neurons.
Key words: glutamate receptor; AMPA receptor; single-channel conductance; RNA editing; alternative splicing; patch-clamp electrophysiology
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