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Volume 17, Number 11, Issue of June 1, 1997 pp. 4236-4242
Copyright ©1997 Society for Neuroscience

A New Form of Inherited Red-Blindness Identified in Zebrafish

Received Dec. 30, 1996; revised Feb. 7, 1997; accepted March 12, 1997.

Susan E. Brockerhoff¹, James B. Hurley², Gregory A. Niemi², and John E. Dowling¹

¹ Department of Molecular and Cellular Biology, The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138, and ² Department of Biochemistry and Howard Hughes Medical Institute, University of Washington, Seattle, Washington 98195

A red-blind zebrafish mutant, partial optokinetic response b (pob), has been isolated by measuring eye movements of larvae in a three-generation screen for recessive mutations affecting the visual system. pob larvae exhibit eye movements in response to rotating black and white stripes illuminated with white light, but they do not move their eyes when the stripes are illuminated with red light. Physiological, immunohistochemical, and in situ hybridization analyses of pob retinas showed a selective loss of red-sensitive cones at 5 days postfertilization (dpf). At 3 dpf, cells expressing red opsin are present, suggesting that red-sensitive cones form initially but then disappear rapidly, whereas other photoreceptors remain. Linkage analysis indicated that the mutation identified in the pob mutant is not at the red opsin locus. Because red opsin is the only known molecule unique to red cones, these data suggest that a novel gene is required for the maintenance or function of red cones.

Key words: zebrafish; retina; red opsin; vision; behavior; mutant

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