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Volume 17, Number 12, Issue of June 15, 1997 pp. 4711-4721
Copyright ©1997 Society for Neuroscience

An ATP-Dependent Inwardly Rectifying Potassium Channel, K_AB-2 (Kir4.1), in Cochlear Stria Vascularis of Inner Ear: Its Specific Subcellular Localization and Correlation with the Formation of Endocochlear Potential

Received Jan. 21, 1997; revised March 17, 1997; accepted March 31, 1997.

Hiroshi Hibino^{1, 2}, Yoshiyuki Horio¹, Atsushi Inanobe⁴, Katsumi Doi², Minoru Ito⁴, Mitsuhiko Yamada¹, Takahiro Gotow³, Yasuo Uchiyama³, Masaru Kawamura⁵, Takeshi Kubo², and Yoshihisa Kurachi^{1, 4}

Departments of ¹ Pharmacology II, ² Otolaryngology, and ³ Anatomy I, Faculty of Medicine, Osaka University, Osaka 565, Japan, ⁴ Department of Cell Biology and Signaling, Yamagata University School of Medicine, Yamagata 990-23, Japan, and ⁵ Department of Biology, University of Occupational and Environmental Health, Kitakyushu 807, Japan

Cochlear endolymph has a highly positive potential of approximately +80 mV. This so-called endocochlear potential (EP) is essential for hearing. Although pivotal roles of K⁺ channels in the formation of EP have been suggested, the types and distribution of K⁺ channels in cochlea have not been characterized. Because EP was depressed by vascular perfusion of Ba²⁺, an inhibitor of inwardly rectifying K⁺ (Kir) channels, but not by either 4-aminopyridine or tetraethylammonium, we examined the expression of Kir channel subunits in cochlear stria vascularis, the tissue that is supposed to play the central role in the generation of positive EP. Of 11 members of the Kir channel family examined with reverse transcription-PCR, we could detect only expression of K_AB-2 (Kir4.1) mRNA in stria vascularis. K_AB-2 immunoreactivity was specifically localized at the basolateral membrane of marginal cells but not in either basal or intermediate cells. Developmental expression of K_AB-2 in marginal cells paralleled formation of EP. Furthermore, deaf mutant mice (viable dominant spotting; W^V/W^V) expressed no K_AB-2 in their marginal cells. These results suggest that K_AB-2 in marginal cells may be critically involved in the generation of positive EP.

Key words: inwardly rectifying potassium channel; endocochlear potential; stria vascularis; vestibule; development; W^V/W^V mice

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