QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (22) Citing Articles via Google Scholar Google Scholar Articles by Wu, A. Articles by Robakis, N. K. Search for Related Content PubMed PubMed Citation Articles by Wu, A. Articles by Robakis, N. K.

 Previous Article  |  Next Article 

Volume 17, Number 13, Issue of July 1, 1997 pp. 4987-4993
Copyright ©1997 Society for Neuroscience

Appican Expression Induces Morphological Changes in C6 Glioma Cells and Promotes Adhesion of Neural Cells to the Extracellular Matrix

Received Feb. 2, 1997; revised April 2, 1997; accepted April 16, 1997.

Anfan Wu, Menelas N. Pangalos, Spiros Efthimiopoulos, Junichi Shioi, and Nikolaos K. Robakis

Department of Psychiatry and Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, New York 10029

Appicans are secreted or cell-associated brain chondroitin sulfate proteoglycans produced by glia cells and containing Alzheimer amyloid precursor protein (APP) as a core protein. Here, we report that rat C6 glioma cells transfected with appican displayed a dramatic change in their phenotypic appearance compared with untransfected cells or cells transfected with APP. Appican-transfected cells lost the round appearance of the untransfected control C6 cells, acquired a flat morphology, and elaborated more processes than control cells. Untransfected, or APP-transfected C6, cells were completely dissociated from their substrate after 40 min of treatment with cell dissociation solution. Under the same conditions, however, <20% of the appican-transfected C6 cells were dissociated from their substrate, suggesting that the appican-transfected glia cells attach more avidly to their substrate than do untransfected or APP transfected control cells. In contrast, appican-transfected fibroblast cells showed no morphological changes and dissociated from their substrate similarly to untransfected fibroblast cells. Extracellular matrix (ECM) prepared from appican-transfected C6 cell cultures contained high levels of appican and was a significantly better substrate for the attachment of C6 cells than ECM from either untransfected or APP-transfected cultures. Furthermore, cell adhesion to ECM was independent of the level of appican expression of the plated cells. ECM from appican-transfected C6 cultures stimulated adhesion of other neural cells including primary astrocytes, Neuro2a neuroblastoma, and PC12 pheochromocytoma, but not fibroblast cells. Conditioned media from appican-transfected C6 cultures failed to promote cell adhesion. Together, these data suggest that secreted appican incorporates into ECM and promotes adhesion of neural cells. Furthermore, our data suggest that the chondroitin sulfate chain engenders APP with novel biological functions.

Key words: Alzheimer disease; appican; APP; cell adhesion; extracellular matrix, proteoglycan

This article has been cited by other articles:

				J. Katahira, T. Miki, K. Takano, M. Maruhashi, M. Uchikawa, T. Tachibana, and Y. Yoneda Nuclear RNA export factor 7 is localized in processing bodies and neuronal RNA granules through interactions with shuttling hnRNPs Nucleic Acids Res., February 2, 2008; 36(2): 616 - 628. [Abstract] [Full Text] [PDF]

				A. Purushothaman, J. Fukuda, S. Mizumoto, G. B. ten Dam, T. H. van Kuppevelt, H. Kitagawa, T. Mikami, and K. Sugahara Functions of Chondroitin Sulfate/Dermatan Sulfate Chains in Brain Development: CRITICAL ROLES OF E AND iE DISACCHARIDE UNITS RECOGNIZED BY A SINGLE CHAIN ANTIBODY GD3G7 J. Biol. Chem., July 6, 2007; 282(27): 19442 - 19452. [Abstract] [Full Text] [PDF]

				P. H. Frederikse and X.-O. Ren Lens Defects and Age-Related Fiber Cell Degeneration in a Mouse Model of Increased A{beta}PP Gene Dosage in Down Syndrome Am. J. Pathol., December 1, 2002; 161(6): 1985 - 1990. [Abstract] [Full Text] [PDF]

				S. S. Deepa, Y. Umehara, S. Higashiyama, N. Itoh, and K. Sugahara Specific Molecular Interactions of Oversulfated Chondroitin Sulfate E with Various Heparin-binding Growth Factors. IMPLICATIONS AS A PHYSIOLOGICAL BINDING PARTNER IN THE BRAIN AND OTHER TISSUES J. Biol. Chem., November 8, 2002; 277(46): 43707 - 43716. [Abstract] [Full Text] [PDF]

				Q. Chen, H. Kimura, and D. Schubert A novel mechanism for the regulation of amyloid precursor protein metabolism J. Cell Biol., July 8, 2002; 158(1): 79 - 89. [Abstract] [Full Text] [PDF]

				L. Baki, P. Marambaud, S. Efthimiopoulos, A. Georgakopoulos, P. Wen, W. Cui, J. Shioi, E. Koo, M. Ozawa, V. L. Friedrich Jr., et al. Presenilin-1 binds cytoplasmic epithelial cadherin, inhibits cadherin/p120 association, and regulates stability and function of the cadherin/catenin adhesion complex PNAS, February 27, 2001; 98(5): 2381 - 2386. [Abstract] [Full Text] [PDF]

				C. Bergsdorf, K. Paliga, S. Kreger, C. L. Masters, and K. Beyreuther Identification of cis-Elements Regulating Exon 15 Splicing of the Amyloid Precursor Protein Pre-mRNA J. Biol. Chem., January 21, 2000; 275(3): 2046 - 2056. [Abstract] [Full Text] [PDF]

				K. Tsuchida, J. Shioi, S. Yamada, G. Boghosian, A. Wu, H. Cai, K. Sugahara, and N. K. Robakis Appican, the Proteoglycan Form of the Amyloid Precursor Protein, Contains Chondroitin Sulfate E in the Repeating Disaccharide Region and 4-O-Sulfated Galactose in the Linkage Region J. Biol. Chem., September 28, 2001; 276(40): 37155 - 37160. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help