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Volume 17, Number 14, Issue of July 15, 1997 pp. 5297-5304
Copyright ©1997 Society for Neuroscience

ATP P_2X Receptors Mediate Fast Synaptic Transmission in the Dorsal Horn of the Rat Spinal Cord

Received Feb. 24, 1997; revised April 29, 1997; accepted May 1, 1997.

Rita Bardoni^{1, 3}, Peter A. Goldstein², C. Justin Lee^{1, 3}, Jianguo G. Gu^{1, 3}, and Amy B. MacDermott^{1, 3}

Departments of ¹ Physiology and Cellular Biophysics, ² Anesthesiology, and the ³ Center for Neurobiology and Behavior, Columbia University, New York, New York 10032

ATP has been proposed to mediate synaptic transmission in the spinal cord dorsal horn, particularly in the pathway carrying nociceptive information. Using transverse spinal cord slices from postnatal rats, we show that EPSCs mediated by P_2X receptors, and presumably activated by synaptically released ATP, are evoked in a subpopulation of spinal cord lamina II neurons, a region known to receive strong input from nociceptive primary afferents. The P_2X receptors on acutely dissociated dorsal horn neurons are nondesensitizing, insensitive to methylene ATP, and show strong but variable sensitivity to the antagonists suramin and pyridoxal-phosphate-6-azophenyl-2,4-disulfonic acid (PPADS). These characteristics are consistent with a heterogeneous population of P_2X receptors, the composition of which includes P_2X2, P_2X4, and P_2X6 receptor subtypes. Our results suggest that ATP-activated P_2X receptors in lamina II of the rat spinal cord may play a role in transmitting or modulating nociceptive information.

Key words: ATP; purinergic receptors; synaptic transmission; spinal cord; rat; patch-clamp technique; slice preparation

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