QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (99) Citing Articles via Google Scholar Google Scholar Articles by Gallo, G. Articles by Letourneau, P. C. Search for Related Content PubMed PubMed Citation Articles by Gallo, G. Articles by Letourneau, P. C. Pubmed/NCBI databases Substance via MeSH

 Previous Article  |  Next Article 

Volume 17, Number 14, Issue of July 15, 1997 pp. 5445-5454
Copyright ©1997 Society for Neuroscience

The trkA Receptor Mediates Growth Cone Turning toward a Localized Source of Nerve Growth Factor

Received March 18, 1997; revised May 5, 1997; accepted May 7, 1997.

Gianluca Gallo¹, Frances B. Lefcort², and Paul C. Letourneau¹

¹ Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, Minnesota 55455, and ² Department of Biology, Montana State University, Bozeman, Montana 59717

We have developed an in vitro system for studying the interaction of chick dorsal root ganglion neuronal growth cones with a localized source of nerve growth factor (NGF) covalently conjugated to polystyrene beads. Growth cones rapidly turned and migrated under NGF-coated beads in a process that involved the initial formation of persistent contact with a bead, followed by directed flow of cytoplasm toward the point of contact. A role for the local activation of the high-affinity NGF receptor trkA was suggested by a strong inhibition of the turning response by (1) the addition of an antibody against the extracellular portion of trkA, (2) the elevation of the background concentration of NGF to saturate trkA, or (3) the presence of a concentration of the drug K252a that inhibits trkA activation. NGF binding to the pan-neurotrophin receptor p75 is also involved but is not required for turning. These data show a new role for both the trkA and the p75 receptors: the mediation of local events in the guidance of nerve growth cones.

Key words: growth cone; nerve growth factor; trkA; p75; guidance; filopodia

This article has been cited by other articles:

				H. Akiyama, T. Matsu-ura, K. Mikoshiba, and H. Kamiguchi Control of Neuronal Growth Cone Navigation by Asymmetric Inositol 1,4,5-Trisphosphate Signals Sci. Signal., July 14, 2009; 2(79): ra34 - ra34. [Abstract] [Full Text] [PDF]

				J. Verburg and P. J. Hollenbeck Mitochondrial Membrane Potential in Axons Increases with Local Nerve Growth Factor or Semaphorin Signaling J. Neurosci., August 13, 2008; 28(33): 8306 - 8315. [Abstract] [Full Text] [PDF]

				L. Taylor, L. Jones, M. H. Tuszynski, and A. Blesch Neurotrophin-3 gradients established by lentiviral gene delivery promote short-distance axonal bridging beyond cellular grafts in the injured spinal cord. J. Neurosci., September 20, 2006; 26(38): 9713 - 9721. [Abstract] [Full Text] [PDF]

				J. Xu, W. J. Rosoff, J. S. Urbach, and G. J. Goodhill Adaptation is not required to explain the long-term response of axons to molecular gradients Development, October 15, 2005; 132(20): 4545 - 4552. [Abstract] [Full Text] [PDF]

				S. Gehler, A. E. Shaw, P. D. Sarmiere, J. R. Bamburg, and P. C. Letourneau Brain-Derived Neurotrophic Factor Regulation of Retinal Growth Cone Filopodial Dynamics Is Mediated through Actin Depolymerizing Factor/Cofilin J. Neurosci., November 24, 2004; 24(47): 10741 - 10749. [Abstract] [Full Text] [PDF]

				P. Lu, H. Yang, L. L. Jones, M. T. Filbin, and M. H. Tuszynski Combinatorial Therapy with Neurotrophins and cAMP Promotes Axonal Regeneration beyond Sites of Spinal Cord Injury J. Neurosci., July 14, 2004; 24(28): 6402 - 6409. [Abstract] [Full Text] [PDF]

				S. Gehler, G. Gallo, E. Veien, and P. C. Letourneau p75 Neurotrophin Receptor Signaling Regulates Growth Cone Filopodial Dynamics through Modulating RhoA Activity J. Neurosci., May 5, 2004; 24(18): 4363 - 4372. [Abstract] [Full Text] [PDF]

				J. K. Atwal, K. K. Singh, M. Tessier-Lavigne, F. D. Miller, and D. R. Kaplan Semaphorin 3F Antagonizes Neurotrophin-Induced Phosphatidylinositol 3-Kinase and Mitogen-Activated Protein Kinase Kinase Signaling: A Mechanism for Growth Cone Collapse J. Neurosci., August 20, 2003; 23(20): 7602 - 7609. [Abstract] [Full Text] [PDF]

				S. R. Chada and P. J. Hollenbeck Mitochondrial movement and positioning in axons: the role of growth factor signaling J. Exp. Biol., June 15, 2003; 206(12): 1985 - 1992. [Abstract] [Full Text] [PDF]

				C. Weinl, U. Drescher, S. Lang, F. Bonhoeffer, and J. Loschinger On the turning of Xenopus retinal axons induced by ephrin-A5 Development, April 15, 2003; 130(8): 1635 - 1643. [Abstract] [Full Text] [PDF]

				V. D. Dontchev and P. C. Letourneau Growth Cones Integrate Signaling from Multiple Guidance Cues J. Histochem. Cytochem., April 1, 2003; 51(4): 435 - 444. [Abstract] [Full Text] [PDF]

				M. E. Brown and P. C. Bridgman Retrograde flow rate is increased in growth cones from myosin IIB knockout mice J. Cell Sci., March 15, 2003; 116(6): 1087 - 1094. [Abstract] [Full Text] [PDF]

				W. Guan, M. A. Puthenveedu, and M. L. Condic Sensory Neuron Subtypes Have Unique Substratum Preference and Receptor Expression before Target Innervation J. Neurosci., March 1, 2003; 23(5): 1781 - 1791. [Abstract] [Full Text] [PDF]

				V. D. Dontchev and P. C. Letourneau Nerve Growth Factor and Semaphorin 3A Signaling Pathways Interact in Regulating Sensory Neuronal Growth Cone Motility J. Neurosci., August 1, 2002; 22(15): 6659 - 6669. [Abstract] [Full Text] [PDF]

				P. H. Ozdinler and R. S. Erzurumlu Slit2, a Branching-Arborization Factor for Sensory Axons in the Mammalian CNS J. Neurosci., June 1, 2002; 22(11): 4540 - 4549. [Abstract] [Full Text] [PDF]

				F. S. Lee and M. V. Chao Activation of Trk neurotrophin receptors in the absence of neurotrophins PNAS, March 1, 2001; (2001) 61020198. [Abstract] [Full Text]

				H. Kong, J. Boulter, J. L. Weber, C. Lai, and M. V. Chao An Evolutionarily Conserved Transmembrane Protein That Is a Novel Downstream Target of Neurotrophin and Ephrin Receptors J. Neurosci., January 1, 2001; 21(1): 176 - 185. [Abstract] [Full Text] [PDF]

				G. E. Spencer, K. Lukowiak, and N. I. Syed Transmitter-Receptor Interactions between Growth Cones of Identified Lymnaea Neurons Determine Target Cell Selection In Vitro J. Neurosci., November 1, 2000; 20(21): 8077 - 8086. [Abstract] [Full Text] [PDF]

				M. I. Romero, N. Rangappa, L. Li, E. Lightfoot, M. G. Garry, and G. M. Smith Extensive Sprouting of Sensory Afferents and Hyperalgesia Induced by Conditional Expression of Nerve Growth Factor in the Adult Spinal Cord J. Neurosci., June 15, 2000; 20(12): 4435 - 4445. [Abstract] [Full Text] [PDF]

				A. F. Ernst, G. Gallo, P. C. Letourneau, and S. C. McLoon Stabilization of Growing Retinal Axons by the Combined Signaling of Nitric Oxide and Brain-Derived Neurotrophic Factor J. Neurosci., February 15, 2000; 20(4): 1458 - 1469. [Abstract] [Full Text] [PDF]

				H.L. Zhang, R.H. Singer, and G.J. Bassell Neurotrophin Regulation of {beta}-Actin mRNA and Protein Localization within Growth Cones J. Cell Biol., October 4, 1999; 147(1): 59 - 70. [Abstract] [Full Text] [PDF]

				J. Kohn, R. S. Aloyz, J. G. Toma, M. Haak-Frendscho, and F. D. Miller Functionally Antagonistic Interactions between the TrkA and p75 Neurotrophin Receptors Regulate Sympathetic Neuron Growth and Target Innervation J. Neurosci., July 1, 1999; 19(13): 5393 - 5408. [Abstract] [Full Text] [PDF]

				G. Gallo and P. C. Letourneau Different Contributions of Microtubule Dynamics and Transport to the Growth of Axons and Collateral Sprouts J. Neurosci., May 15, 1999; 19(10): 3860 - 3873. [Abstract] [Full Text] [PDF]

				C. M. Isbister and T. P. O'Connor Filopodial Adhesion Does Not Predict Growth Cone Steering Events In Vivo J. Neurosci., April 1, 1999; 19(7): 2589 - 2600. [Abstract] [Full Text] [PDF]

				G. S. Walsh, K. M. Krol, and M. D. Kawaja Absence of the p75 Neurotrophin Receptor Alters the Pattern of Sympathosensory Sprouting in the Trigeminal Ganglia of Mice Overexpressing Nerve Growth Factor J. Neurosci., January 1, 1999; 19(1): 258 - 273. [Abstract] [Full Text] [PDF]

				H. H. D. Lam, A. Bhardwaj, M. T. O'Connell, D. F. Hanley, R. J. Traystman, and M. V. Sofroniew Nerve growth factor rapidly suppresses basal, NMDA-evoked, and AMPA-evoked nitric oxide synthase activity in rat hippocampus in vivo PNAS, September 1, 1998; 95(18): 10926 - 10931. [Abstract] [Full Text] [PDF]

				G. Gallo and P. C. Letourneau Localized Sources of Neurotrophins Initiate Axon Collateral Sprouting J. Neurosci., July 15, 1998; 18(14): 5403 - 5414. [Abstract] [Full Text] [PDF]

				Q. Wang and J. Q. Zheng cAMP-Mediated Regulation of Neurotrophin-Induced Collapse of Nerve Growth Cones J. Neurosci., July 1, 1998; 18(13): 4973 - 4984. [Abstract] [Full Text] [PDF]

				F. S. Lee and M. V. Chao Activation of Trk neurotrophin receptors in the absence of neurotrophins PNAS, March 13, 2001; 98(6): 3555 - 3560. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help