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Volume 17, Number 14, Issue of July 15, 1997 pp. 5466-5479
Copyright ©1997 Society for NeuroscienceImmunohistochemical Localization of Netrin-1 in the Embryonic Chick Nervous System
Received Nov. 18, 1996; revised March 17, 1997; accepted April 11, 1997.
A. John MacLennan, Diana L. McLaurin, Lianne Marks, Emily N. Vinson, Marylynn Pfeifer, Susan V. Szulc, Marieta B. Heaton, and Nancy Lee Department of Neuroscience, University of Florida Brain Institute, University of Florida College of Medicine, Gainesville, Florida 32610-0244
Netrin-1 has profound in vitro effects on the growth properties of vertebrate embryonic axons. In addition, netrin-1 mRNA is found in the floor plate of the embryonic nervous system, an intermediate target of many axons, including commissural axons that are affected by netrin-1 in vitro. Moreover, genetic studies of netrin-1 homologs in Caenorhabditis elegans and Drosophila implicate these proteins in commissure formation. We raised polyclonal antisera that recognize chick netrin-1 in fixed tissue sections. The antisera were used to immunohistochemically map netrin-1 in the embryonic spinal cord, brain, and retina. The relationship between netrin-1 localization and the growth of pioneering axons suggests roles for netrin-1 in the regulation of circumferential, commissural, and longitudinal axon growth in the spinal cord and brain. The data also suggest that the primary or sole effect of netrin-1 on pioneering spinal cord commissural axons is haptotactic. Furthermore, the pattern of netrin-1 localization raises the possibility that this protein helps mediate neuronal migration in the spinal cord, brain, and retina.
Key words: axon guidance; brain; circumferential; commissural; floor plate; immunohistochemistry; retina; spinal cord
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