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Volume 17, Number 15, Issue of August 1, 1997 pp. 5858-5867
Copyright ©1997 Society for NeuroscienceQuantitative Ultrastructural Analysis of Hippocampal Excitatory Synapses
Received March 25, 1997; revised May 5, 1997; accepted May 19, 1997.
Thomas Schikorski and Charles F. Stevens Molecular Neurobiology Laboratory and Howard Hughes Medical Institute, The Salk Institute, La Jolla, California 92037
From three-dimensional reconstructions of CA1 excitatory synapses in the rodent hippocampus and in culture, we have estimated statistical distributions of active zone and postsynaptic density (PSD) sizes (average area ~0.04 µm2), the number of active zones per bouton (usually one), the number of docked vesicles per active zone (~10), and the total number of vesicles per bouton (~200), and we have determined relationships between these quantities, all of which vary from synapse to synapse but are highly correlated. These measurements have been related to synaptic physiology. In particular, we propose that the distribution of active zone areas can account for the distribution of synaptic release probabilities and that each active zone constitutes a release site as identified in the standard quantal theory attributable to .
Key words: synaptic vesicle; active zone; release; statistical distribution; hippocampus; release probability
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