QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (51) Citing Articles via Google Scholar Google Scholar Articles by Seamans, J. K. Articles by Yang, C. R. Search for Related Content PubMed PubMed Citation Articles by Seamans, J. K. Articles by Yang, C. R.

 Previous Article  |  Next Article 

Volume 17, Number 15, Issue of August 1, 1997 pp. 5936-5948
Copyright ©1997 Society for Neuroscience

Contributions of Voltage-Gated Ca²⁺ Channels in the Proximal versus Distal Dendrites to Synaptic Integration in Prefrontal Cortical Neurons

Received Feb. 13, 1997; revised May 5, 1997; accepted May 9, 1997.

Jeremy K. Seamans¹, Natalia A. Gorelova¹, and Charles R. Yang^{1, 2}

Departments of ¹ Psychology and ² Psychiatry, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada

The electrogenesis of synaptically activated dendritic Ca²⁺-mediated potentials, which may contribute to synaptic signal integration in pyramidal cells, was examined in rat layers V-VI prefrontal cortical (PFC) neurons in vitro. Intrasomatically recorded suprathreshold synaptic responses evoked by stimulation of the distal dendrites were attenuated by focal Cd²⁺ application to the proximal apical dendritic stem (100-200 µm from soma), but not to the apical dendritic tuft (>500 µm from soma). With use of intracellular QX-314 and Cs⁺ to block Na⁺ and K⁺ currents, intrasomatic recordings revealed that the Cd²⁺-induced attenuation of synaptic responses was attributable to the blockade of a dendritic Ca²⁺-mediated "hump" potential and high-threshold Ca²⁺ spike activated by NMDA EPSPs. The hump potential was not blocked by bath application of Ni²⁺ (100 µM) but was blocked by focal application of Cd²⁺ to the proximal but not distal apical dendrites, suggesting that it was generated by Ca²⁺ channels located in the proximal dendrites. Direct patch-clamp recordings made from the distal apical tuft of layers V-VI PFC neurons revealed that layers I-II synaptic stimulation or intradendritic depolarizing current pulses evoked tetrodotoxin- and QX-314-sensitive Na⁺ spikes. Unlike in the stem of the apical dendrite, Ca²⁺ spikes were not easily evoked in the distal apical tuft when Na⁺ channels were blocked. When triggered, the Cd²⁺-sensitive Ca²⁺ spikes in the dendritic tuft were nonregenerative and had very high activation thresholds (approximately +10 mV). These results suggested that the high voltage-activated Ca²⁺ potentials that amplify distal EPSPs are primarily generated in the proximal stem of the apical dendrite and not within the fine dendritic branches of the apical tuft of layers V-VI PFC neurons.

Key words: dendrites; PFC; amplification; calcium; NMDA; EPSP; apical tuft; electrophysiology

This article has been cited by other articles:

				J. W. Worrell and R. B. Levine Characterization of Voltage-Dependent Ca2+ Currents in Identified Drosophila Motoneurons In Situ J Neurophysiol, August 1, 2008; 100(2): 868 - 878. [Abstract] [Full Text] [PDF]

				P. J. Sjostrom, E. A. Rancz, A. Roth, and M. Hausser Dendritic Excitability and Synaptic Plasticity Physiol Rev, April 1, 2008; 88(2): 769 - 840. [Abstract] [Full Text] [PDF]

				A. M. I. Barth, E. S. Vizi, T. Zelles, and B. Lendvai {alpha}2-Adrenergic Receptors Modify Dendritic Spike Generation Via HCN Channels in the Prefrontal Cortex J Neurophysiol, January 1, 2008; 99(1): 394 - 401. [Abstract] [Full Text] [PDF]

				D. C. Rotaru, D. A. Lewis, and G. Gonzalez-Burgos Dopamine D1 receptor activation regulates sodium channel-dependent EPSP amplification in rat prefrontal cortex pyramidal neurons J. Physiol., June 15, 2007; 581(3): 981 - 1000. [Abstract] [Full Text] [PDF]

				L. Chen, J. D. Bohanick, M. Nishihara, J. K. Seamans, and C. R. Yang Dopamine D1/5 Receptor-Mediated Long-Term Potentiation of Intrinsic Excitability in Rat Prefrontal Cortical Neurons: Ca2+-Dependent Intracellular Signaling J Neurophysiol, March 1, 2007; 97(3): 2448 - 2464. [Abstract] [Full Text] [PDF]

				A. R. Mercer, P. Kloppenburg, and J. G. Hildebrand Plateau Potentials in Developing Antennal-Lobe Neurons of the Moth, Manduca sexta J Neurophysiol, April 1, 2005; 93(4): 1949 - 1958. [Abstract] [Full Text] [PDF]

				N. A. Otmakhova and J. E. Lisman Contribution of Ih and GABAB to Synaptically Induced Afterhyperpolarizations in CA1: A Brake on the NMDA Response J Neurophysiol, October 1, 2004; 92(4): 2027 - 2039. [Abstract] [Full Text] [PDF]

				C. E. Young and C. R. Yang Dopamine D1/D5 Receptor Modulates State-Dependent Switching of Soma-Dendritic Ca2+ Potentials via Differential Protein Kinase A and C Activation in Rat Prefrontal Cortical Neurons J. Neurosci., January 7, 2004; 24(1): 8 - 23. [Abstract] [Full Text] [PDF]

				A. T. Gulledge and G. J. Stuart Action Potential Initiation and Propagation in Layer 5 Pyramidal Neurons of the Rat Prefrontal Cortex: Absence of Dopamine Modulation J. Neurosci., December 10, 2003; 23(36): 11363 - 11372. [Abstract] [Full Text] [PDF]

				R Vergara, C Rick, S Hernandez-Lopez, J A Laville, J N Guzman, E Galarraga, D J Surmeier, and J Bargas Spontaneous voltage oscillations in striatal projection neurons in a rat corticostriatal slice J. Physiol., November 15, 2003; 553(1): 169 - 182. [Abstract] [Full Text] [PDF]

				E. S. Fortune and G. J. Rose Voltage-Gated Na+ Channels Enhance the Temporal Filtering Properties of Electrosensory Neurons in the Torus J Neurophysiol, August 1, 2003; 90(2): 924 - 929. [Abstract] [Full Text] [PDF]

				L. Chen, M. Muhlhauser, and C. R. Yang Glycine Tranporter-1 Blockade Potentiates NMDA-Mediated Responses in Rat Prefrontal Cortical Neurons In Vitro and In Vivo J Neurophysiol, February 1, 2003; 89(2): 691 - 703. [Abstract] [Full Text] [PDF]

				G. E. Stutzmann, F. M. LaFerla, and I. Parker Ca2+ Signaling in Mouse Cortical Neurons Studied by Two-Photon Imaging and Photoreleased Inositol Triphosphate J. Neurosci., February 1, 2003; 23(3): 758 - 765. [Abstract] [Full Text] [PDF]

				L. Chen and C. R. Yang Interaction of Dopamine D1 and NMDA Receptors Mediates Acute Clozapine Potentiation of Glutamate EPSPs in Rat Prefrontal Cortex J Neurophysiol, May 1, 2002; 87(5): 2324 - 2336. [Abstract] [Full Text] [PDF]

				N. A. Otmakhova, N. Otmakhov, and J. E. Lisman Pathway-Specific Properties of AMPA and NMDA-Mediated Transmission in CA1 Hippocampal Pyramidal Cells J. Neurosci., February 15, 2002; 22(4): 1199 - 1207. [Abstract] [Full Text] [PDF]

				G. Gonzalez-Burgos and G. Barrionuevo Voltage-Gated Sodium Channels Shape Subthreshold EPSPs in Layer 5 Pyramidal Neurons From Rat Prefrontal Cortex J Neurophysiol, October 1, 2001; 86(4): 1671 - 1684. [Abstract] [Full Text] [PDF]

				A. Frick, W. Zieglgansberger, and H.-U. Dodt Glutamate Receptors Form Hot Spots on Apical Dendrites of Neocortical Pyramidal Neurons J Neurophysiol, September 1, 2001; 86(3): 1412 - 1421. [Abstract] [Full Text] [PDF]

				P. Vetter, A. Roth, and M. Hausser Propagation of Action Potentials in Dendrites Depends on Dendritic Morphology J Neurophysiol, February 1, 2001; 85(2): 926 - 937. [Abstract] [Full Text] [PDF]

				F. F. De-Miguel, M. Vargas-Caballero, and E. Garcia-Perez Spread of synaptic potentials through electrical synapses in Retzius neurones of the leech J. Exp. Biol., January 10, 2001; 204(19): 3241 - 3250. [Abstract] [Full Text] [PDF]

				J. K. Seamans, D. Durstewitz, B. R. Christie, C. F. Stevens, and T. J. Sejnowski Dopamine D1/D5 receptor modulation of excitatory synaptic inputs to layer V prefrontal cortex neurons PNAS, December 22, 2000; (2000) 11518798. [Abstract] [Full Text]

				D. Durstewitz, J. K. Seamans, and T. J. Sejnowski Dopamine-Mediated Stabilization of Delay-Period Activity in a Network Model of Prefrontal Cortex J Neurophysiol, March 1, 2000; 83(3): 1733 - 1750. [Abstract] [Full Text] [PDF]

				J. Magistretti, D. S Ragsdale, and A. Alonso Direct demonstration of persistent Na+ channel activity in dendritic processes of mammalian cortical neurones J. Physiol., December 15, 1999; 521(3): 629 - 636. [Abstract] [Full Text] [PDF]

				N. L. Golding, H.-y. Jung, T. Mickus, and N. Spruston Dendritic Calcium Spike Initiation and Repolarization Are Controlled by Distinct Potassium Channel Subtypes in CA1 Pyramidal Neurons J. Neurosci., October 15, 1999; 19(20): 8789 - 8798. [Abstract] [Full Text] [PDF]

				R. Wessel, W. B. Kristan Jr, and D. Kleinfeld Supralinear Summation of Synaptic Inputs by an Invertebrate Neuron: Dendritic Gain Is Mediated by an "Inward Rectifier" K+ Current J. Neurosci., July 15, 1999; 19(14): 5875 - 5888. [Abstract] [Full Text] [PDF]

				D. Durstewitz, M. Kelc, and O. Gunturkun A Neurocomputational Theory of the Dopaminergic Modulation of Working Memory Functions J. Neurosci., April 1, 1999; 19(7): 2807 - 2822. [Abstract] [Full Text] [PDF]

				P. Saltiel, M. C. Tresch, and E. Bizzi Spinal Cord Modular Organization and Rhythm Generation: An NMDA Iontophoretic Study in the Frog J Neurophysiol, November 1, 1998; 80(5): 2323 - 2339. [Abstract] [Full Text] [PDF]

				G. C. Tombaugh Intracellular pH Buffering Shapes Activity-Dependent Ca2+ Dynamics in Dendrites of CA1 Interneurons J Neurophysiol, October 1, 1998; 80(4): 1702 - 1712. [Abstract] [Full Text] [PDF]

				E. D. Schutter Dendritic Voltage and Calcium-Gated Channels Amplify the Variability of Postsynaptic Responses in a Purkinje Cell Model J Neurophysiol, August 1, 1998; 80(2): 504 - 519. [Abstract] [Full Text] [PDF]

				J. Zahrt, J. R. Taylor, R. G. Mathew, and A. F. T. Arnsten Supranormal Stimulation of D1 Dopamine Receptors in the Rodent Prefrontal Cortex Impairs Spatial Working Memory Performance J. Neurosci., November 1, 1997; 17(21): 8528 - 8535. [Abstract] [Full Text] [PDF]

				J. K. Seamans, D. Durstewitz, B. R. Christie, C. F. Stevens, and T. J. Sejnowski Dopamine D1/D5 receptor modulation of excitatory synaptic inputs to layer V prefrontal cortex neurons PNAS, January 2, 2001; 98(1): 301 - 306. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help