QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (24) Citing Articles via Google Scholar Google Scholar Articles by White, M. G. Articles by Meriney, S. D. Search for Related Content PubMed PubMed Citation Articles by White, M. G. Articles by Meriney, S. D. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CALCIUM COMPOUNDS CALCIUM, ELEMENTAL PYRROLE

 Previous Article  |  Next Article 

Volume 17, Number 16, Issue of August 15, 1997 pp. 6302-6313
Copyright ©1997 Society for Neuroscience

Developmental Changes in Calcium Current Pharmacology and Somatostatin Inhibition in Chick Parasympathetic Neurons

Received March 13, 1997; revised May 27, 1997; accepted May 30, 1997.

Michael G. White, Mark A. Crumling, and Stephen D. Meriney

Department of Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15260

Voltage-dependent calcium (Ca²⁺) currents were characterized and modulatory effects of somatostatin were measured in acutely dissociated chick ciliary ganglion neurons at embryonic stages 34, 37, and 40. This developmental time period coincides with the period of synapse formation between ciliary ganglion neurons and peripheral eye muscles. At all three developmental stages Ca²⁺ current could be blocked almost completely by combined application of -CgTX GVIA and nitrendipine. At young embryonic ages there was significant overlap in sensitivity, with ~75% of the current sensitive to either blocker applied independently. By stage 40, there was very little or no overlap in sensitivity, with ~75% of the current blocked by -CgTX GVIA (N-type) and 30% blocked by nitrendipine (L-type). These data are consistent with earlier findings that the pharmacology of acetylcholine release from ciliary ganglion nerve terminals changes during development from sensitivity to both dihydropyridines and -CgTX GVIA to selective sensitivity to -CgTX GVIA (). Somatostatin reduced Ca²⁺ current by 50-60% at all three developmental stages. At early developmental stages somatostatin receptors coupled predominantly to the current that was sensitive to both -CgTX GVIA and nitrendipine. By stage 40, somatostatin primarily inhibited classically defined N-type current (selectively sensitive to -CgTX GVIA). Thus, somatostatin receptor coupling to Ca²⁺ channels persisted throughout development as Ca²⁺ current pharmacology changed.

Key words: calcium channel; somatostatin modulation; development; chick ciliary ganglion; FPL 64176; -CgTX GVIA; dihydropyridines

This article has been cited by other articles:

				J. D. King Jr. and S. D. Meriney Proportion of N-Type Calcium Current Activated by Action Potential Stimuli J Neurophysiol, December 1, 2005; 94(6): 3762 - 3770. [Abstract] [Full Text] [PDF]

				G. Piccoli, U. Rutishauser, and J. L. Bruses N-Cadherin Juxtamembrane Domain Modulates Voltage-Gated Ca2+ Current via RhoA GTPase and Rho-Associated Kinase J. Neurosci., December 1, 2004; 24(48): 10918 - 10923. [Abstract] [Full Text] [PDF]

				Q. Li, A. Lau, T. J. Morris, L. Guo, C. B. Fordyce, and E. F. Stanley A Syntaxin 1, G{alpha}o, and N-Type Calcium Channel Complex at a Presynaptic Nerve Terminal: Analysis by Quantitative Immunocolocalization J. Neurosci., April 21, 2004; 24(16): 4070 - 4081. [Abstract] [Full Text] [PDF]

				Q.-Q. Sun, J. R. Huguenard, and D. A. Prince Somatostatin Inhibits Thalamic Network Oscillations In Vitro: Actions on the GABAergic Neurons of the Reticular Nucleus J. Neurosci., July 1, 2002; 22(13): 5374 - 5386. [Abstract] [Full Text] [PDF]

				D. E. Artim and S. D. Meriney G-Protein-Modulated Ca2+ Current With Slowed Activation Does Not Alter the Kinetics of Action Potential-Evoked Ca2+ Current J Neurophysiol, November 1, 2000; 84(5): 2417 - 2425. [Abstract] [Full Text] [PDF]

				B. Buchwald, M. de Baets, G.-J. Luijckx, and K. V. Toyka Neonatal Guillain-Barre syndrome: Blocking antibodies transmitted from mother to child Neurology, October 1, 1999; 53(6): 1246 - 1246. [Abstract] [Full Text]

				L. Polo-Parada and G. Pilar kappa - and µ-Opioids Reverse the Somatostatin Inhibition of Ca2+ Currents in Ciliary and Dorsal Root Ganglion Neurons J. Neurosci., July 1, 1999; 19(13): 5213 - 5227. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help