 |
 Previous Article
Volume 17, Number 16,
Issue of August 15, 1997
pp. 6483-6491
Copyright ©1997 Society for Neuroscience
Low-Frequency Stimulation of Afferent A -Fibers Induces
Long-Term Depression at Primary Afferent Synapses with Substantia
Gelatinosa Neurons in the Rat
Received May 5, 1997; accepted June 4, 1997.
J. Sandkühler1, 2,
J. G. Chen2,
G. Cheng1, and
M. Randi 1
1 Department of Veterinary Physiology and Pharmacology,
Iowa State University, Ames, Iowa 50011, and 2 Institute of
Physiology II, University of Heidelberg, 69120 Heidelberg, Germany
Impulses in primary afferent nerve fibers may produce short- or
long-lasting modifications in spinal nociception. Here we have
identified a robust long-term depression (LTD) of synaptic transmission
in substantia gelatinosa neurons that can be induced by low-frequency
stimulation of primary afferent A -fibers. Synaptic transmission
between dorsal root afferents and neurons in the substantia gelatinosa
of the spinal cord dorsal horn was examined by intracellular recording
in a transverse slice dorsal root preparation of rat spinal cord.
Conditioning stimulation of dorsal roots with 900 pulses given at 1 Hz
(10 V, 0.1 msec) produced LTD of EPSP amplitudes in substantia
gelatinosa neurons to 41 ± 10% of control that lasted for at
least 2 hr. When A- and C-fibers were recruited, conditioning
stimulation was as effective as A-fiber stimulation alone. After LTD,
synaptic strength could be increased to its original level by applying
a second, high-frequency tetanic stimulus to the dorsal root,
indicating that LTD is reversible and not attributable to damage of
individual synapses. Bath application of the GABAA receptor
antagonist bicuculline and glycine receptor antagonist strychnine did
not affect LTD. When NMDA receptors were blocked by bath application of
D-2-amino-5-phosphonovaleric acid, LTD was abolished or
strongly reduced. Loading substantia gelatinosa neurons with
Ca2+ chelator BAPTA also blocked or reduced LTD.
After incubation of slices with calyculin A, a selective and membrane
permeable inhibitor of protein phosphatases 1 and 2A, LTD was not
attenuated. We propose that this form of LTD may be relevant for
long-lasting segmental antinociception after afferent stimulation.
Key words:
primary afferents;
synaptic transmission;
spinal cord;
substantia gelatinosa;
pain;
antinociception;
afferent stimulation;
long-term depression;
NMDA;
BAPTA;
calyculin A
This article has been cited by other articles:
|
|
|
|
 
O. Ozsoylar, D. Akcali, P. Cizmeci, A. Babacan, A. Cahana, and H. Bolay
Percutaneous Pulsed Radiofrequency Reduces Mechanical Allodynia in a Neuropathic Pain Model
Anesth. Analg.,
October 1, 2008;
107(4):
1406 - 1411.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Sun, T. J. Gan, J. W. Dubose, and A. S. Habib
Acupuncture and related techniques for postoperative pain: a systematic review of randomized controlled trials
Br. J. Anaesth.,
August 1, 2008;
101(2):
151 - 160.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Price, M. H. Rashid, M. Millecamps, R. Sanoja, J. M. Entrena, and F. Cervero
Decreased Nociceptive Sensitization in Mice Lacking the Fragile X Mental Retardation Protein: Role of mGluR1/5 and mTOR
J. Neurosci.,
December 19, 2007;
27(51):
13958 - 13967.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Hansen, T. Klein, W. Magerl, and R.-D. Treede
Psychophysical Evidence for Long-Term Potentiation of C-Fiber and A{delta}-Fiber Pathways in Humans by Analysis of Pain Descriptors
J Neurophysiol,
March 1, 2007;
97(3):
2559 - 2563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. W. Grau, E. D. Crown, A. R. Ferguson, S. N. Washburn, M. A. Hook, and R. C. Miranda
Instrumental learning within the spinal cord: underlying mechanisms and implications for recovery after injury.
Behav Cogn Neurosci Rev,
December 1, 2006;
5(4):
191 - 239.
[Abstract]
[PDF]
|
|
|
|
|
|
|
I. Kondo, J. C. G. Marvizon, B. Song, F. Salgado, S. Codeluppi, X.-Y. Hua, and T. L. Yaksh
Inhibition by Spinal {micro}- and {delta}-Opioid Agonists of Afferent-Evoked Substance P Release
J. Neurosci.,
April 6, 2005;
25(14):
3651 - 3660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ellrich
Electric Low-Frequency Stimulation of the Tongue Induces Long-Term Depression of the Jaw-Opening Reflex in Anesthetized Mice
J Neurophysiol,
December 1, 2004;
92(6):
3332 - 3337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Kato, H. Furue, T. Katafuchi, T. Yasaka, Y. Iwamoto, and M. Yoshimura
Electrophysiological mapping of the nociceptive inputs to the substantia gelatinosa in rat horizontal spinal cord slices
J. Physiol.,
October 1, 2004;
560(1):
303 - 315.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Klein, W. Magerl, H.-C. Hopf, J. Sandkuhler, and R.-D. Treede
Perceptual Correlates of Nociceptive Long-Term Potentiation and Long-Term Depression in Humans
J. Neurosci.,
January 28, 2004;
24(4):
964 - 971.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Song and J. C. G. Marvizon
Dorsal Horn Neurons Firing at High Frequency, But Not Primary Afferents, Release Opioid Peptides that Produce {micro}-Opioid Receptor Internalization in the Rat Spinal Cord
J. Neurosci.,
October 8, 2003;
23(27):
9171 - 9184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Gu
P2X Receptor-Mediated Modulation of Sensory Transmission to the Spinal Cord Dorsal Horn
Neuroscientist,
October 1, 2003;
9(5):
370 - 378.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. P. Mauderli, C. J. Vierck Jr., R. L. Cannon, A. Rodrigues, and C. Shen
Relationships Between Skin Temperature and Temporal Summation of Heat and Cold Pain
J Neurophysiol,
July 1, 2003;
90(1):
100 - 109.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Genzen and D. S. McGehee
Short- and long-term enhancement of excitatory transmission in the spinal cord dorsal horn by nicotinic acetylcholine receptors
PNAS,
May 27, 2003;
100(11):
6807 - 6812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Song and J. C. G. Marvizon
Peptidases Prevent {micro}-Opioid Receptor Internalization in Dorsal Horn Neurons by Endogenously Released Opioids
J. Neurosci.,
March 1, 2003;
23(5):
1847 - 1858.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Stener-Victorin, M. Wikland, U. Waldenstrom, and T. Lundeberg
Alternative treatments in reproductive medicine: much ado about nothing: Acupuncture--a method of treatment in reproductive medicine: lack of evidence of an effect does not equal evidence of the lack of an effect
Hum. Reprod.,
August 1, 2002;
17(8):
1942 - 1946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Schwartzman, J. Grothusen, T. R. Kiefer, and P. Rohr
Neuropathic Central Pain: Epidemiology, Etiology, and Treatment Options
Arch Neurol,
October 1, 2001;
58(10):
1547 - 1550.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Garraway and S. Hochman
Serotonin Increases the Incidence of Primary Afferent-Evoked Long-Term Depression in Rat Deep Dorsal Horn Neurons
J Neurophysiol,
May 1, 2001;
85(5):
1864 - 1872.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Woolf and M. W. Salter
Neuronal Plasticity: Increasing the Gain in Pain
Science,
June 9, 2000;
288(5472):
1765 - 1768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ikeda, T. Asai, and K. Murase
Robust Changes of Afferent-Induced Excitation in the Rat Spinal Dorsal Horn After Conditioning High-Frequency Stimulation
J Neurophysiol,
April 1, 2000;
83(4):
2412 - 2420.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Levinsson, X.-L. Luo, H. Holmberg, and J. Schouenborg
Developmental Tuning in a Spinal Nociceptive System: Effects of Neonatal Spinalization
J. Neurosci.,
December 1, 1999;
19(23):
10397 - 10403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ikeda, T. Asai, M. Randic, and K. Murase
Robust Suppression of Afferent-Induced Excitation in the Rat Spinal Dorsal Horn After Conditioning Low-Frequency Stimulation
J Neurophysiol,
October 1, 1999;
82(4):
1957 - 1964.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Baba, T. P. Doubell, and C. J. Woolf
Peripheral Inflammation Facilitates Abeta Fiber-Mediated Synaptic Input to the Substantia Gelatinosa of the Adult Rat Spinal Cord
J. Neurosci.,
January 15, 1999;
19(2):
859 - 867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J Bastian
Plasticity of feedback inputs in the apteronotid electrosensory system
J. Exp. Biol.,
January 5, 1999;
202(10):
1327 - 1337.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. Bastian
Modulation of Calcium-Dependent Postsynaptic Depression Contributes to an Adaptive Sensory Filter
J Neurophysiol,
December 1, 1998;
80(6):
3352 - 3355.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bardoni, P. C. Magherini, and A. B. MacDermott
NMDA EPSCs at Glutamatergic Synapses in the Spinal Cord Dorsal Horn of the Postnatal Rat
J. Neurosci.,
August 15, 1998;
18(16):
6558 - 6567.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-G. Liu and J. Sandkuhler
Characterization of Long-Term Potentiation of C-Fiber-Evoked Potentials in Spinal Dorsal Horn of Adult Rat: Essential Role of NK1 and NK2 Receptors
J Neurophysiol,
October 1, 1997;
78(4):
1973 - 1982.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
.gif?ad=17923&adview=true)
