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Volume 17, Number 17, Issue of September 1, 1997 pp. 6621-6628
Copyright ©1997 Society for Neuroscience

T-Type Ca2+ Current Properties Are Not Modified by Ca2+ Channel beta  Subunit Depletion in Nodosus Ganglion Neurons

Received April 15, 1997; revised June 16, 1997; accepted June 19, 1997.

Régis C. Lambert1, Yves Maulet1, Jérôme Mouton1, Ruth Beattie2, Steve Volsen2, Michel De Waard3, and Anne Feltz1

1 Laboratoire de Neurobiologie Cellulaire, UPR 9009 Centre National de la Recherche Scientifique, 67084 Strasbourg, France, 2 Lilly Research Center Limited, Windlesham, Surrey, GU20 PH, United Kingdom, and 3 Laboratoire de Neurobiologie des Canaux Ioniques, U-374 Institut National de la Santé et de la Recherche Médicale, 13910 Marseille, France

At the molecular level, our knowledge of the low voltage-activated Ca2+ channel (T-type) has made little progress. Using an antisense strategy, we investigated the possibility that the T-type channels have a structure similar to high voltage-activated Ca2+ channels. It is assumed that high voltage-activated channels are made of at least three components: a pore forming alpha 1 subunit combined with a cytoplasmic modulatory beta  subunit and a primarily extracellular alpha 2delta subunit. We have examined the effect of transfecting cranial primary sensory neurons with generic anti-beta antisense oligonucleotides. We show that in this cell type, blocking expression of all known beta  gene products does not affect T-type current, although it greatly decreases the current amplitude of high voltage-activated channels and modifies their voltage dependence. This suggests that beta  subunits are likely not constitutive of T-type Ca2+ channels in this cell type.

Key words: T-type Ca2+ current; HVA Ca2+ currents; sensory neurons; beta subunit; antisense oligonucleotide; polyethylenimine




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