WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience MBF Stereo Investigator
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (72)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ullian, E. M.
Right arrow Articles by Sargent, P. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ullian, E. M.
Right arrow Articles by Sargent, P. B.

 Previous Article  |  Next Article 

Volume 17, Number 19, Issue of October 1, 1997 pp. 7210-7219
Copyright ©1997 Society for Neuroscience

Rapid Synaptic Transmission in the Avian Ciliary Ganglion Is Mediated by Two Distinct Classes of Nicotinic Receptors

Received May 6, 1997; revised July 8, 1997; accepted July 10, 1997.

Erik M. Ullian1, J. Michael McIntosh2, and Peter B. Sargent1

1 Neuroscience Graduate Program and Departments of Stomatology and Physiology, University of California, San Francisco, California 94143, and 2 Departments of Psychiatry and Biology, University of Utah, Salt Lake City, Utah 84112

We analyzed the kinetics and pharmacology of EPSCs in two kinds of neurons in the embryonic avian ciliary ganglion. Whole-cell voltage-clamp recordings revealed that the singly innervated ciliary neurons had large-amplitude (1.5-8.0 nA) EPSCs that could be classified according to the kinetics of their falling phases. Most of the neurons responded with an EPSC the falling phase of which followed a double exponential time course with time constants of ~1 and 10 msec. The EPSCs of the remaining ciliary neurons followed a single time constant (~8 msec). Multiple innervated choroid neurons had smaller-amplitude responses (0.2-1.5 nA when all inputs were activated) that appeared to contain only a slowly decaying component (tau  = 12 msec). The fast and slow components of EPSC decay seen in most ciliary neurons could be pharmacologically isolated with two toxins against nicotinic acetylcholine receptors (AChRs). The fast component was blocked by 50 nM alpha -bungarotoxin (alpha -BuTx), which binds alpha 7-subunit-containing AChRs. The slow component was selectively blocked by 50 nM alpha -conotoxin MII (alpha -CTx-MII), which blocks mammalian AChRs containing an alpha 3/beta 2 subunit interface. A combination of both alpha -BuTx and alpha -CTx-MII abolished nearly all evoked current. Similar pharmacological results were found for ciliary neurons with monoexponentially decaying EPSCs and for choroid neurons. These results suggest that nerve-evoked transmitter acts on at least two different populations of AChRs on autonomic motor neurons in the ciliary ganglion.

Key words: acetylcholine receptors; excitatory postsynaptic current; alpha -bungarotoxin; alpha -conotoxin MII; alpha 7; alpha 3; beta 2




This article has been cited by other articles:


Home page
 J. Immunol.Home page
S. Razani-Boroujerdi, R. T. Boyd, M. I. Davila-Garcia, J. S. Nandi, N. C. Mishra, S. P. Singh, J. C. Pena-Philippides, R. Langley, and M. L. Sopori
T Cells Express {alpha}7-Nicotinic Acetylcholine Receptor Subunits That Require a Functional TCR and Leukocyte-Specific Protein Tyrosine Kinase for Nicotine-Induced Ca2+ Response
J. Immunol., September 1, 2007; 179(5): 2889 - 2898.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Rassadi, A. Krishnaswamy, B. Pie, R. McConnell, M. H. Jacob, and E. Cooper
A Null Mutation for the {alpha}3 Nicotinic Acetylcholine (ACh) Receptor Gene Abolishes Fast Synaptic Activity in Sympathetic Ganglia and Reveals That ACh Output from Developing Preganglionic Terminals Is Regulated in an Activity-Dependent Retrograde Manner
J. Neurosci., September 14, 2005; 25(37): 8555 - 8566.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. S. Coggan, T. M. Bartol, E. Esquenazi, J. R. Stiles, S. Lamont, M. E. Martone, D. K. Berg, M. H. Ellisman, and T. J. Sejnowski
Evidence for Ectopic Neurotransmission at a Neuronal Synapse
Science, July 15, 2005; 309(5733): 446 - 451.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
J. Deck, S. Bibevski, T. Gnecchi-Ruscone, V. Bellina, N. Montano, and M. E. Dunlap
{alpha}7-Nicotinic acetylcholine receptor subunit is not required for parasympathetic control of the heart in the mouse
Physiol Genomics, June 16, 2005; 22(1): 86 - 92.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
Q. Nai, J. M. McIntosh, and J. F. Margiotta
Relating Neuronal Nicotinic Acetylcholine Receptor Subtypes Defined by Subunit Composition and Channel Function
Mol. Pharmacol., February 1, 2003; 63(2): 311 - 324.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. L. Brumwell, J. L. Johnson, and M. H. Jacob
Extrasynaptic alpha 7-Nicotinic Acetylcholine Receptor Expression in Developing Neurons Is Regulated by Inputs, Targets, and Activity
J. Neurosci., September 15, 2002; 22(18): 8101 - 8109.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Fucile, E. Palma, A. Martinez-Torres, R. Miledi, and F. Eusebi
The single-channel properties of human acetylcholine alpha 7 receptors are altered by fusing alpha 7 to the green fluorescent protein
PNAS, March 19, 2002; 99(6): 3956 - 3961.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. D. Shoop, E. Esquenazi, N. Yamada, M. H. Ellisman, and D. K. Berg
Ultrastructure of a Somatic Spine Mat for Nicotinic Signaling in Neurons
J. Neurosci., February 1, 2002; 22(3): 748 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS, March 22, 2001; (2001) 81553598.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
R. D. Shoop, K. T. Chang, M. H. Ellisman, and D. K. Berg
Synaptically Driven Calcium Transients via Nicotinic Receptors on Somatic Spines
J. Neurosci., February 1, 2001; 21(3): 771 - 781.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
J. S. Cameron and S. E. Dryer
BK-Type KCa Channels in Two Parasympathetic Cell Types: Differences in Kinetic Properties and Developmental Expression
J Neurophysiol, December 1, 2000; 84(6): 2767 - 2776.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
M. E. McNerney, D. Pardi, P. C. Pugh, Q. Nai, and J. F. Margiotta
Expression and Channel Properties of alpha -Bungarotoxin-Sensitive Acetylcholine Receptors on Chick Ciliary and Choroid Neurons
J Neurophysiol, September 1, 2000; 84(3): 1314 - 1329.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Bibevski, Y. Zhou, J. M. McIntosh, R. E. Zigmond, and M. E. Dunlap
Functional Nicotinic Acetylcholine Receptors That Mediate Ganglionic Transmission in Cardiac Parasympathetic Neurons
J. Neurosci., July 1, 2000; 20(13): 5076 - 5082.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. D. Shoop, N. Yamada, and D. K. Berg
Cytoskeletal Links of Neuronal Acetylcholine Receptors Containing alpha 7 Subunits
J. Neurosci., June 1, 2000; 20(11): 4021 - 4029.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
P. Whiteaker, J. M. McIntosh, S. Luo, A. C. Collins, and M. J. Marks
125I-alpha -Conotoxin MII Identifies a Novel Nicotinic Acetylcholine Receptor Population in Mouse Brain
Mol. Pharmacol., May 1, 2000; 57(5): 913 - 925.
[Abstract] [Full Text]

Home page
 J. Neurosci.Home page
Q.-s. Liu and D. K. Berg
Actin Filaments and the Opposing Actions of CaM Kinase II and Calcineurin in Regulating alpha 7-Containing Nicotinic Receptors on Chick Ciliary Ganglion Neurons
J. Neurosci., December 1, 1999; 19(23): 10280 - 10288.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
Q.-S. Liu and D. K. Berg
Extracellular Calcium Regulates Responses of Both alpha 3- and alpha 7-Containing Nicotinic Receptors on Chick Ciliary Ganglion Neurons
J Neurophysiol, September 1, 1999; 82(3): 1124 - 1132.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. T. Chang and D. K. Berg
Nicotinic Acetylcholine Receptors Containing alpha 7 Subunits Are Required for Reliable Synaptic Transmission In Situ
J. Neurosci., May 15, 1999; 19(10): 3701 - 3710.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
B. M. WILLIAMS, M. K. TEMBURNI, S. BERTRAND, D. BERTRAND, and M. H. JACOB
The Long Cytoplasmic Loop of the a3 Subunit Targets Specific nAChR Subtypes to Synapses on Neurons in Vivo
Ann. N.Y. Acad. Sci., April 30, 1999; 868(1): 640 - 644.
[Full Text] [PDF]

Home page
 J. Neurosci.Home page
A. L. Obaid, T. Koyano, J. Lindstrom, T. Sakai, and B. M. Salzberg
Spatiotemporal Patterns of Activity in an Intact Mammalian Network with Single-Cell Resolution: Optical Studies of Nicotinic Activity in an Enteric Plexus
J. Neurosci., April 15, 1999; 19(8): 3073 - 3093.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R. D. Shoop, M. E. Martone, N. Yamada, M. H. Ellisman, and D. K. Berg
Neuronal Acetylcholine Receptors with alpha 7 Subunits Are Concentrated on Somatic Spines for Synaptic Signaling in Embryonic Chick Ciliary Ganglia
J. Neurosci., January 15, 1999; 19(2): 692 - 704.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
E. M. Blumenthal, R. D. Shoop, and D. K. Berg
Developmental Changes in the Nicotinic Responses of Ciliary Ganglion Neurons
J Neurophysiol, January 1, 1999; 81(1): 111 - 120.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
J. Cuevas and D. K. Berg
Mammalian Nicotinic Receptors with alpha 7 Subunits That Slowly Desensitize and Rapidly Recover from alpha -Bungarotoxin Blockade
J. Neurosci., December 15, 1998; 18(24): 10335 - 10344.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. G. Lopez, C. Montiel, C. J. Herrero, E. Garcia-Palomero, I. Mayorgas, J. M. Hernandez-Guijo, M. Villarroya, R. Olivares, L. Gandia, J. M. McIntosh, et al.
Unmasking the functions of the chromaffin cell alpha 7 nicotinic receptor by using short pulses of acetylcholine and selective blockers
PNAS, November 24, 1998; 95(24): 14184 - 14189.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. Luo, J. M. Kulak, G. E. Cartier, R. B. Jacobsen, D. Yoshikami, B. M. Olivera, and J. M. McIntosh
alpha -Conotoxin AuIB Selectively Blocks alpha 3beta 4 Nicotinic Acetylcholine Receptors and Nicotine-Evoked Norepinephrine Release
J. Neurosci., November 1, 1998; 18(21): 8571 - 8579.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Wang, M. E. Nelson, A. Kuryatov, F. Olale, J. Cooper, K. Keyser, and J. Lindstrom
Chronic Nicotine Treatment Up-regulates Human alpha 3beta 2 but Not alpha 3beta 4 Acetylcholine Receptors Stably Transfected in Human Embryonic Kidney Cells
J. Biol. Chem., October 30, 1998; 273(44): 28721 - 28732.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. J. Frazier, A. V. Buhler, J. L. Weiner, and T. V. Dunwiddie
Synaptic Potentials Mediated via alpha -Bungarotoxin-Sensitive Nicotinic Acetylcholine Receptors in Rat Hippocampal Interneurons
J. Neurosci., October 15, 1998; 18(20): 8228 - 8235.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
K. A. Radcliffe and J. A. Dani
Nicotinic Stimulation Produces Multiple Forms of Increased Glutamatergic Synaptic Transmission
J. Neurosci., September 15, 1998; 18(18): 7075 - 7083.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Q.-s. Liu, H. Kawai, and D. K. Berg
beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons
PNAS, April 10, 2001; 98(8): 4734 - 4739.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-