 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 17, Number 20, Issue of October 15, 1997 pp. 7763-7783
Copyright ©1997 Society for NeuroscienceRegulation of Neuroblast Cell-Cycle Kinetics Plays a Crucial Role in the Generation of Unique Features of Neocortical Areas
Received May 29, 1997; revised July 21, 1997; accepted July 24, 1997.
Franck Polleux, Colette Dehay, Bétrice Moraillon, and Henry Kennedy Institut National de la Santé et de la Recherche Médicale U371-Cerveau et Vision, 69675 BRON Cedex, France
Cortical neurons are generated in the germinal zones lining the ventricles before migrating predominantly radially. To investigate regional differences in the cell-cycle kinetics of neuroblasts, pulse [3H]-thymidine injections were made throughout corticogenesis, and labeled neuron counts were compared in areas 3, 6, 17, and 18a in the adult mouse. The relationship between height in the cortex and intensity of autoradiographic signal distinguishes first generation and subsequent generations of neurons. This provides the mitotic history of defined sets of neurons and is a powerful tool for analyzing areal differences in cell-cycle kinetics. The infragranular laminar labeling indices of different generations show significant differences in areas 3 and 6. The labeling index of first generation neurons shows that the rate of neuron production is higher in area 3 than in area 6. This increased generation rate in area 3 was accompanied by two major changes. First, computation of the labeling index of the subsequent generation neurons (which reflects percentages of precursors in S-phase at the moment of the pulse) indicates a shorter cell cycle in area 3. Second, the total population of labeled neurons contains a higher proportion of first generation neurons in area 3, implying a higher leaving fraction in this area. Computer simulations of these areal differences of cell-cycle kinetics generate neuron numbers that are in close agreement with published data. Altogether these findings reveal an early regionalization of the ventricular zone that serves to generate unique features of future cortical areas.
Key words: cell-cycle dynamics; corticogenesis; neocortex; development; rodents; visual, somatosensory, and motor systems; proliferation; tritiated thymidine; modelization
This article has been cited by other articles:
J. M. Gohlke, W. C. Griffith, and E. M. Faustman
Computational Models of Neocortical Neuronogenesis and Programmed Cell Death in the Developing Mouse, Monkey, and Human
Cereb Cortex, October 1, 2007; 17(10): 2433 - 2442.
[Abstract] [Full Text] [PDF]
M. Gotz and L. Sommer
Cortical development: the art of generating cell diversity
Development, August 1, 2005; 132(15): 3327 - 3332.
[Abstract] [Full Text] [PDF]
A. Bellion, M. Wassef, and C. Metin
Early Differences in Axonal Outgrowth, Cell Migration and GABAergic Differentiation Properties between the Dorsal and Lateral Cortex
Cereb Cortex, February 1, 2003; 13(2): 203 - 214.
[Abstract] [Full Text] [PDF]
A. Lukaszewicz, P. Savatier, V. Cortay, H. Kennedy, and C. Dehay
Contrasting Effects of Basic Fibroblast Growth Factor and Neurotrophin 3 on Cell Cycle Kinetics of Mouse Cortical Stem Cells
J. Neurosci., August 1, 2002; 22(15): 6610 - 6622.
[Abstract] [Full Text] [PDF]
F. Polleux, C. Dehay, A. Goffinet, and H. Kennedy
Pre- and Post-mitotic Events Contribute to the Progressive Acquisition of Area-specific Connectional Fate in the Neocortex
Cereb Cortex, November 1, 2001; 11(11): 1027 - 1039.
[Abstract] [Full Text] [PDF]
C. Dehay, P. Savatier, V. Cortay, and H. Kennedy
Cell-Cycle Kinetics of Neocortical Precursors Are Influenced by Embryonic Thalamic Axons
J. Neurosci., January 1, 2001; 21(1): 201 - 214.
[Abstract] [Full Text] [PDF]
T. F. Haydar, F. Wang, M. L. Schwartz, and P. Rakic
Differential Modulation of Proliferation in the Neocortical Ventricular and Subventricular Zones
J. Neurosci., August 1, 2000; 20(15): 5764 - 5774.
[Abstract] [Full Text] [PDF]
J. M. Soria and A. Fairen
Cellular Mosaics in the Rat Marginal Zone Define an Early Neocortical Territorialization
Cereb Cortex, April 1, 2000; 10(4): 400 - 412.
[Abstract] [Full Text] [PDF]
T. Takahashi, T. Goto, S. Miyama, R. S. Nowakowski, and V. S. Caviness Jr
Sequence of Neuron Origin and Neocortical Laminar Fate: Relation to Cell Cycle of Origin in the Developing Murine Cerebral Wall
J. Neurosci., December 1, 1999; 19(23): 10357 - 10371.
[Abstract] [Full Text] [PDF]
J. L.R. Rubenstein, S. Anderson, L. Shi, E. Miyashita-Lin, A. Bulfone, and R. Hevner
Genetic Control of Cortical Regionalization and Connectivity
Cereb Cortex, September 1, 1999; 9(6): 524 - 532.
[Abstract] [Full Text] [PDF]
Y. Gitton, M. Cohen-Tannoudji, and M. Wassef
Role of Thalamic Axons in the Expression of H-2Z1, a Mouse Somatosensory Cortex Specific Marker
Cereb Cortex, September 1, 1999; 9(6): 611 - 620.
[Abstract] [Full Text] [PDF]
M. J. Donoghue and P. Rakic
Molecular Evidence for the Early Specification of Presumptive Functional Domains in the Embryonic Primate Cerebral Cortex
J. Neurosci., July 15, 1999; 19(14): 5967 - 5979.
[Abstract] [Full Text] [PDF]
M. F. Mehler and J. A. Kessler
Progenitor Cell Biology: Implications for Neural Regeneration
Arch Neurol, July 1, 1999; 56(7): 780 - 784.
[Abstract] [Full Text] [PDF]
Y. Gitton, M. Cohen-Tannoudji, and M. Wassef
Specification of Somatosensory Area Identity in Cortical Explants
J. Neurosci., June 15, 1999; 19(12): 4889 - 4898.
[Abstract] [Full Text] [PDF]
F. Polleux, C. Dehay, and H. Kennedy
Neurogenesis and Commitment of Corticospinal Neurons in reeler
J. Neurosci., December 1, 1998; 18(23): 9910 - 9923.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|