 |
 Previous Article | Next Article 
Volume 17, Number 20,
Issue of October 15, 1997
pp. 7974-7987
Copyright ©1997 Society for Neuroscience
Both Neuropeptide Y and Serotonin Are Necessary for
Entrainment of Circadian Rhythms in Mice by Daily Treadmill Running
Schedules
Received May 8, 1997; revised July 25, 1997; accepted July 29, 1997.
Elliott G. Marchant,
Neil V. Watson, and
Ralph E. Mistlberger
Department of Psychology, Simon Fraser University, Burnaby, British
Columbia V5A 1S6, Canada
This study investigated the role of the suprachiasmatic nucleus
(SCN) circadian pacemaker and its neuropeptide Y (NPY) and serotonin
(5-HT) afferents in entrainment (synchronization) of mouse circadian
rhythms by treadmill running. Blind C57BL/6j mice were run in
treadmills for 3 hr/d for 3-10 weeks after receiving radio-frequency
lesions of the SCN or the intergeniculate leaflet (IGL, the source of
SCN NPY) or infusions of the 5-HT neurotoxin 5,7-DHT into the SCN area.
Of 25 intact mice, 22 entrained and three showed period ( , the mean
duration of the circadian cycle) modulations to scheduled running.
Arrhythmic SCN-ablated mice did not synchronize to scheduled running in
a way suggestive of circadian pacemaker mediation. Of 15 mice with IGL
lesions, only two with partial lesions entrained. Mice with complete
IGL lesions (five), confirmed by immunocytochemistry, showed no
entrainment or changes. Of 19 mice with 5-HT lesions, only two with
partial lesions entrained. All but two mice with complete (10) or
nearly complete (4) 5-HT denervation, confirmed by immunocytochemistry, showed modulations during the treadmill schedule. Failure to entrain was not explained by group differences in before the treadmill schedules. The results indicate that the SCN and both NPY and
5-HT are necessary for entrainment to 24 hr schedules of forced running
but that complete loss of 5-HT does not prevent modulations of
pacemaker motion by behavioral stimuli. Treadmill entrainment in mice
may involve synergistic interactions between 5-HT and NPY afferents at
some site within the circadian system.
Key words:
circadian rhythms;
entrainment;
neuropeptide Y;
serotonin;
intergeniculate leaflet;
raphe nuclei;
suprachiasmatic
nucleus;
treadmill running
This article has been cited by other articles:
|
|
|
|
 
M. C. Antle, R. Sterniczuk, V. M. Smith, and K. Hagel
Non-Photic Modulation of Phase Shifts to Long Light Pulses
J Biol Rhythms,
December 1, 2007;
22(6):
524 - 533.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. M. Fuller, J. J. Gooley, and C. B. Saper
Neurobiology of the Sleep-Wake Cycle: Sleep Architecture, Circadian Regulation, and Regulatory Feedback
J Biol Rhythms,
December 1, 2006;
21(6):
482 - 493.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Thankachan and B. Rusak
Juxtacellular Recording/Labeling Analysis of Physiological and Anatomical Characteristics of Rat Intergeniculate Leaflet Neurons
J. Neurosci.,
October 5, 2005;
25(40):
9195 - 9204.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Mendoza, C. Graff, H. Dardente, P. Pevet, and E. Challet
Feeding Cues Alter Clock Gene Oscillations and Photic Responses in the Suprachiasmatic Nuclei of Mice Exposed to a Light/Dark Cycle
J. Neurosci.,
February 9, 2005;
25(6):
1514 - 1522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Marinesco, K. E. Kolkman, and T. J. Carew
Serotonergic Modulation in Aplysia. I. Distributed Serotonergic Network Persistently Activated by Sensitizing Stimuli
J Neurophysiol,
October 1, 2004;
92(4):
2468 - 2486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. R. Castillo, K. J. Hochstetler, R. J. Tavernier Jr, D. M. Greene, and A. Bult-Ito
Entrainment of the master circadian clock by scheduled feeding
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2004;
287(3):
R551 - R555.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. K. Barger, K. P. Wright Jr., R. J. Hughes, and C. A. Czeisler
Daily exercise facilitates phase delays of circadian melatonin rhythm in very dim light
Am J Physiol Regulatory Integrative Comp Physiol,
June 1, 2004;
286(6):
R1077 - R1084.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Novak and H. E. Albers
Novel phase-shifting effects of GABAA receptor activation in the suprachiasmatic nucleus of a diurnal rodent
Am J Physiol Regulatory Integrative Comp Physiol,
May 1, 2004;
286(5):
R820 - R825.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. E. Mistlberger, M. C. Antle, I. C. Webb, M. Jones, J. Weinberg, and M. S. Pollock
Circadian clock resetting by arousal in Syrian hamsters: the role of stress and activity
Am J Physiol Regulatory Integrative Comp Physiol,
October 1, 2003;
285(4):
R917 - R925.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Menet, P. Vuillez, M. Saboureau, and P. Pevet
Inhibition of hibernation by exercise is not affected by intergeniculate leaflets lesion in hamsters
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2003;
285(3):
R690 - R700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. D. Glass, G. H. Grossman, L. Farnbauch, and L. DiNardo
Midbrain Raphe Modulation of Nonphotic Circadian Clock Resetting and 5-HT Release in the Mammalian Suprachiasmatic Nucleus
J. Neurosci.,
August 20, 2003;
23(20):
7451 - 7460.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Antle, M. D. Ogilvie, G. E. Pickard, and R. E. Mistlberger
Response of the Mouse Circadian System to Serotonin 1A/2/7 Agonists in vivo: Surprisingly Little
J Biol Rhythms,
April 1, 2003;
18(2):
145 - 158.
[Abstract]
[PDF]
|
|
|
|
|
|
|
R. E. Mistlberger, J. Belcourt, and M. C. Antle
Circadian Clock Resetting by Sleep Deprivation without Exercise in Syrian Hamsters: Dark Pulses Revisited
J Biol Rhythms,
June 1, 2002;
17(3):
227 - 237.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. C. Ehlen, G. H. Grossman, and J. D. Glass
In Vivo Resetting of the Hamster Circadian Clock by 5-HT7 Receptors in the Suprachiasmatic Nucleus
J. Neurosci.,
July 15, 2001;
21(14):
5351 - 5357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Smale, T. McElhinny, J. Nixon, B. Gubik, and S. Rose
Patterns of Wheel Running Are Related to Fos Expression in Neuropeptide-Y-Containing Neurons in the Intergeniculate Leaflet of Arvicanthis niloticus
J Biol Rhythms,
April 1, 2001;
16(2):
163 - 172.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. J. H. Kas and D. M. Edgar
Scheduled Voluntary Wheel Running Activity Modulates Free-Running Circadian Body Temperature Rhythms in Octodon degus
J Biol Rhythms,
February 1, 2001;
16(1):
66 - 75.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J. D. Glass, S. D. Tardif, R. Clements, and N. Mrosovsky
Photic and nonphotic circadian phase resetting in a diurnal primate, the common marmoset
Am J Physiol Regulatory Integrative Comp Physiol,
January 1, 2001;
280(1):
R191 - R197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. Antle and R. E. Mistlberger
Circadian Clock Resetting by Sleep Deprivation without Exercise in the Syrian Hamster
J. Neurosci.,
December 15, 2000;
20(24):
9326 - 9332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. G. Marchant and L. P. Morin
The Hamster Circadian Rhythm System Includes Nuclei of the Subcortical Visual Shell
J. Neurosci.,
December 1, 1999;
19(23):
10482 - 10493.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Flett and C. S. Colwell
Serotonin Modulation of Calcium Transients in Cells in the Suprachiasmatic Nucleus
J Biol Rhythms,
October 1, 1999;
14(5):
354 - 363.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. E. Pickard, B. N. Smith, M. Belenky, M. A. Rea, F. E. Dudek, and P. J. Sollars
5-HT1B Receptor-Mediated Presynaptic Inhibition of Retinal Input to the Suprachiasmatic Nucleus
J. Neurosci.,
May 15, 1999;
19(10):
4034 - 4045.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. E. Dudley, L. A. Dinardo, and J. D. Glass
In Vivo Assessment of the Midbrain Raphe Nuclear Regulation of Serotonin Release in the Hamster Suprachiasmatic Nucleus
J Neurophysiol,
April 1, 1999;
81(4):
1469 - 1477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Kohler, A. Kalkowski, and F. Wollnik
Serotonin Agonist Quipazine Induces Photic-Like Phase Shifts of the Circadian Activity Rhythm and c-Fos Expression in the Rat Suprachiasmatic Nucleus
J Biol Rhythms,
April 1, 1999;
14(2):
131 - 140.
[Abstract]
[PDF]
|
|
|
|
|
|
|
K. Edelstein and S. Amir
The Role of the Intergeniculate Leaflet in Entrainment of Circadian Rhythms to a Skeleton Photoperiod
J. Neurosci.,
January 1, 1999;
19(1):
372 - 380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Solberg, T. H. Horton, and F. W. Turek
Circadian rhythms and depression: effects of exercise in an animal model
Am J Physiol Regulatory Integrative Comp Physiol,
January 1, 1999;
276(1):
R152 - R161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Meyer-Bernstein and L. P. Morin
Destruction of Serotonergic Neurons in the Median Raphe Nucleus Blocks Circadian Rhythm Phase Shifts to Triazolam but Not to Novel Wheel Access
J Biol Rhythms,
December 1, 1998;
13(6):
494 - 505.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E. Challet, K. Scarbrough, P. D. Penev, and F. W. Turek
Roles of Suprachiasmatic Nuclei and Intergeniculate Leaflets in Mediating the Phase-Shifting Effects of a Serotonergic Agonist and Their Photic Modulation during Subjective Day
J Biol Rhythms,
October 1, 1998;
13(5):
410 - 421.
[Abstract]
[PDF]
|
|
|
|
