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Volume 17, Number 21, Issue of November 1, 1997 pp. 8061-8073
Copyright ©1997 Society for Neuroscience

Caenorhabditis elegans rab-3 Mutant Synapses Exhibit Impaired Function and Are Partially Depleted of Vesicles

Received March 12, 1997; revised Aug. 7, 1997; accepted Aug. 11, 1997.

Michael L. Nonet1, 2, Jane E. Staunton2, Michael P. Kilgard1, Tim Fergestad4, Erika Hartwieg3, H. Robert Horvitz3, Erik M. Jorgensen4, and Barbara J. Meyer1

1 Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, 2 Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110, 3 Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, and 4 Department of Biology, University of Utah, Salt Lake City, Utah 84112

Rab molecules regulate vesicular trafficking in many different exocytic and endocytic transport pathways in eukaryotic cells. In neurons, rab3 has been proposed to play a crucial role in regulating synaptic vesicle release. To elucidate the role of rab3 in synaptic transmission, we isolated and characterized Caenorhabditis elegans rab-3 mutants. Similar to the mouse rab3A mutants, these mutants survived and exhibited only mild behavioral abnormalities. In contrast to the mouse mutants, synaptic transmission was perturbed in these animals. Extracellular electrophysiological recordings revealed that synaptic transmission in the pharyngeal nervous system was impaired. Furthermore, rab-3 animals were resistant to the acetylcholinesterase inhibitor aldicarb, suggesting that cholinergic transmission was generally depressed. Last, synaptic vesicle populations were redistributed in rab-3 mutants. In motor neurons, vesicle populations at synapses were depleted to 40% of normal levels, whereas in intersynaptic regions of the axon, vesicle populations were elevated. On the basis of the morphological defects at neuromuscular junctions, we postulate that RAB-3 may regulate recruitment of vesicles to the active zone or sequestration of vesicles near release sites.

Key words: small GTP-binding proteins; exocytosis; rab3; synaptic vesicle proteins; Caenorhabditis elegans; mutants




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Home page
 J. Cell Sci.Home page
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