QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (54) Citing Articles via Google Scholar Google Scholar Articles by Rangwala, F. Articles by Salman, and William N. Green, S. B. Search for Related Content PubMed PubMed Citation Articles by Rangwala, F. Articles by Salman, and William N. Green, S. B.

 Previous Article  |  Next Article 

Volume 17, Number 21, Issue of November 1, 1997 pp. 8201-8212
Copyright ©1997 Society for Neuroscience

Neuronal -Bungarotoxin Receptors Differ Structurally from Other Nicotinic Acetylcholine Receptors

Received May 30, 1997; revised Aug. 13, 1997; accepted Aug. 21, 1997.

Fatima Rangwala, Renaldo C. Drisdel, Sergey Rakhilin, Elizabeth Ko, Pramod Atluri, Amy B. Harkins, Aaron P. Fox, and Suleiman B. Salman, and William N. Green

Department of Pharmacological and Physiological Sciences, University of Chicago, Chicago, IL 60637

We have characterized the -bungarotoxin receptors (BgtRs) found on the cell surface of undifferentiated pheochromocytoma (PC12) cells. The PC12 cells express a homogeneous population of 7-containing receptors that bind -Bgt with high affinity (K_d = 94 pM). The BgtRs mediate most of the response elicited by nicotine, because the BgtR-specific antagonists methyllycaconitine and -Bgt block ~90% of the whole-cell current. The binding of nicotinic agonists to cell-surface BgtRs was highly cooperative with four different agonists showing Hill coefficients in the range of 2.3-2.4. A similar agonist binding cooperativity was observed for BgtR homomers formed from chimeric 7/5HT3 subunits expressed in tsA 201 cells. Two classes of agonist binding sites, in the ratio of 4:1 for PC12 cell BgtRs and 3:1 for 7/5HT3 BgtRs, were revealed by bromoacetylcholine alkylation of the reduced sites on both PC12 BgtRs and 7/5HT3 BgtRs. We conclude from this data that PC12 BgtRs and 7/5HT3 homomers contain at least three distinguishable agonist binding sites and thus are different from other nicotinic receptors.

Key words: nicotine; -bungarotoxin; pheochromocytoma; nicotinic receptors; electrophysiology; pharmacology

This article has been cited by other articles:

				H. Walsh, A. P. Govind, R. Mastro, J. C. Hoda, D. Bertrand, Y. Vallejo, and W. N. Green Up-regulation of Nicotinic Receptors by Nicotine Varies with Receptor Subtype J. Biol. Chem., March 7, 2008; 283(10): 6022 - 6032. [Abstract] [Full Text] [PDF]

				C. Lopes, E. F. R. Pereira, H.-Q. Wu, P. Purushottamachar, V. Njar, R. Schwarcz, and E. X. Albuquerque Competitive Antagonism between the Nicotinic Allosteric Potentiating Ligand Galantamine and Kynurenic Acid at {alpha}7* Nicotinic Receptors J. Pharmacol. Exp. Ther., July 1, 2007; 322(1): 48 - 58. [Abstract] [Full Text] [PDF]

				S. J. Lansdell, V. J. Gee, P. C. Harkness, A. I. Doward, E. R. Baker, A. J. Gibb, and N. S. Millar RIC-3 Enhances Functional Expression of Multiple Nicotinic Acetylcholine Receptor Subtypes in Mammalian Cells Mol. Pharmacol., November 1, 2005; 68(5): 1431 - 1438. [Abstract] [Full Text] [PDF]

				A. Cheng, N. A. McDonald, and C. N. Connolly Cell Surface Expression of 5-Hydroxytryptamine Type 3 Receptors Is Promoted by RIC-3 J. Biol. Chem., June 10, 2005; 280(23): 22502 - 22507. [Abstract] [Full Text] [PDF]

				R. C. Drisdel, E. Manzana, and W. N. Green The Role of Palmitoylation in Functional Expression of Nicotinic {alpha}7 Receptors J. Neurosci., November 17, 2004; 24(46): 10502 - 10510. [Abstract] [Full Text] [PDF]

				E. R. Baker, R. Zwart, E. Sher, and N. S. Millar Pharmacological Properties of {alpha}9{alpha}10 Nicotinic Acetylcholine Receptors Revealed by Heterologous Expression of Subunit Chimeras Mol. Pharmacol., February 1, 2004; 65(2): 453 - 460. [Abstract] [Full Text] [PDF]

				H. Tsuneki, R. Salas, and J. A Dani Mouse muscle denervation increases expression of an {alpha}7 nicotinic receptor with unusual pharmacology J. Physiol., February 15, 2003; 547(1): 169 - 179. [Abstract] [Full Text] [PDF]

				M. Ellison, J. M. McIntosh, and B. M. Olivera alpha -Conotoxins ImI and ImII. SIMILAR alpha 7 NICOTINIC RECEPTOR ANTAGONISTS ACT AT DIFFERENT SITES J. Biol. Chem., January 3, 2003; 278(2): 757 - 764. [Abstract] [Full Text] [PDF]

				P. C. Harkness and N. S. Millar Changes in Conformation and Subcellular Distribution of alpha 4beta 2 Nicotinic Acetylcholine Receptors Revealed by Chronic Nicotine Treatment and Expression of Subunit Chimeras J. Neurosci., December 1, 2002; 22(23): 10172 - 10181. [Abstract] [Full Text] [PDF]

				C. L. Brumwell, J. L. Johnson, and M. H. Jacob Extrasynaptic alpha 7-Nicotinic Acetylcholine Receptor Expression in Developing Neurons Is Regulated by Inputs, Targets, and Activity J. Neurosci., September 15, 2002; 22(18): 8101 - 8109. [Abstract] [Full Text] [PDF]

				S. S Khiroug, P. C Harkness, P. W Lamb, S. N Sudweeks, L. Khiroug, N. S Millar, and J. L Yakel Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels J. Physiol., April 15, 2002; 540(2): 425 - 434. [Abstract] [Full Text] [PDF]

				Q.-s. Liu, H. Kawai, and D. K. Berg beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons PNAS, March 22, 2001; (2001) 81553598. [Abstract] [Full Text]

				M. E. McNerney, D. Pardi, P. C. Pugh, Q. Nai, and J. F. Margiotta Expression and Channel Properties of alpha -Bungarotoxin-Sensitive Acetylcholine Receptors on Chick Ciliary and Choroid Neurons J Neurophysiol, September 1, 2000; 84(3): 1314 - 1329. [Abstract] [Full Text] [PDF]

				J. Cuevas, A. L Roth, and D. K Berg Two distinct classes of functional {alpha}7-containing nicotinic receptor on rat superior cervical ganglion neurons J. Physiol., June 15, 2000; 525(3): 735 - 746. [Abstract] [Full Text] [PDF]

				R. C. Drisdel and W. N. Green Neuronal alpha -Bungarotoxin Receptors Are alpha 7 Subunit Homomers J. Neurosci., January 1, 2000; 20(1): 133 - 139. [Abstract] [Full Text] [PDF]

				S. T. Cooper, P. C. Harkness, E. R. Baker, and N. S. Millar Up-regulation of Cell-surface alpha 4beta 2 Neuronal Nicotinic Receptors by Lower Temperature and Expression of Chimeric Subunits J. Biol. Chem., September 17, 1999; 274(38): 27145 - 27152. [Abstract] [Full Text] [PDF]

				L. K. Lyford and R. L. Rosenberg Cell-free Expression and Functional Reconstitution of Homo-oligomeric alpha 7 Nicotinic Acetylcholine Receptors into Planar Lipid Bilayers J. Biol. Chem., September 3, 1999; 274(36): 25675 - 25681. [Abstract] [Full Text] [PDF]

				S. Rakhilin, R. C. Drisdel, D. Sagher, D. S. McGehee, Y. Vallejo, and W. N. Green {alpha}-Bungarotoxin Receptors Contain {alpha}7 Subunits in Two Different Disulfide-bonded Conformations J. Cell Biol., July 12, 1999; 146(1): 203 - 218. [Abstract] [Full Text] [PDF]

				W. N. Green and C. P. Wanamaker Formation of the Nicotinic Acetylcholine Receptor Binding Sites J. Neurosci., August 1, 1998; 18(15): 5555 - 5564. [Abstract] [Full Text] [PDF]

				P. A. Quiram and S. M. Sine Structural Elements in alpha -Conotoxin ImI Essential for Binding to Neuronal alpha 7 Receptors J. Biol. Chem., May 1, 1998; 273(18): 11007 - 11011. [Abstract] [Full Text] [PDF]

				Q.-s. Liu, H. Kawai, and D. K. Berg beta -Amyloid peptide blocks the response of alpha 7-containing nicotinic receptors on hippocampal neurons PNAS, April 10, 2001; 98(8): 4734 - 4739. [Abstract] [Full Text] [PDF]

				S. S. Khiroug, P. C. Harkness, P. W. Lamb, S. N. Sudweeks, L. Khiroug, N. S. Millar, and J. L. Yakel Rat nicotinic ACh receptor {alpha}7 and {beta}2 subunits co-assemble to form functional heteromeric nicotinic receptor channels J. Physiol., February 22, 2002; (2002) 200101384. [Abstract] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help