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Volume 17, Number 21, Issue of November 1, 1997 pp. 8234-8245
Copyright ©1997 Society for NeuroscienceCharacterization of Delayed Rectifier Kv Channels in Oligodendrocytes and Progenitor Cells
Received March 10, 1997; revised Aug. 5, 1997; accepted Aug. 13, 1997.
Bernard Attali1, Ning Wang2, Alexandra Kolot1, Alexander Sobko1, Vera Cherepanov1, and Betty Soliven2 1 Department of Neurobiology, The Weizmann Institute of Science, Rehovot 76100, Israel, and 2 Department of Neurology and Committee on Neurobiology, The Brain Research Institute, University of Chicago, Chicago, Illinois 60637
We examined the molecular identity of K+ channel genes underlying the delayed rectifier (IK) in differentiated cultured oligodendrocytes (OLGs) and oligodendrocyte progenitor (OP) cells. Using reverse transcription-PCR cloning, we found that OP cells and OLGs expressed multiple Kv transcripts, namely Kv1.2, Kv1.4, Kv.1.5, and Kv1.6. Immunocytochemical and Western blot analyses revealed that Kv1.5 and Kv1.6 as well as Kv1.2 and Kv1.4 channel proteins could be detected in these cells, but definitive evidence for functional K+ channel expression was obtained only for the Kv1.5 channel. In addition, mRNA and immunoreactive protein levels of both Kv1.5 and Kv1.6 channels were significantly lower in differentiated OLGs when compared with levels in OP cells. Proliferation of OP cells was inhibited by K+ channel blockers, but not by incubation with either Kv1.5 or Kv1.6 antisense oligonucleotides. We conclude that (1) IK in OP cells and OLGs is encoded partly by Kv1.5 subunits, possibly forming heteromultimeric channels with Kv1.6 or other Kv subunits; and (2) inhibition of Kv1.5 or Kv1.6 channel expression alone does not prevent mitogenesis. Concomitant inhibition of other Kv channels underlying IK may be necessary for OP cells to exit from cell cycle.
Key words: ion channels; delayed rectifier; glia; oligodendrocyte progenitors; proliferation
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