 |
||||
|
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 17, Number 21, Issue of November 1, 1997 pp. 8363-8375
Copyright ©1997 Society for NeuroscienceGlutamate Transporter Protein Subtypes Are Expressed Differentially during Rat CNS Development
Received May 27, 1997; revised Aug. 15, 1997; accepted Aug. 20, 1997.
Akiko Furuta1, Jeffrey D. Rothstein1, 2, and Lee J. Martin2, 3 Departments of 1 Neurology, 2 Neuroscience, and 3 Pathology, Division of Neuropathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287
Extracellular glutamate concentrations are regulated by glial and neuronal transporter proteins. Four glutamate transporter subtypes have been identified in rat brain; GLAST and GLT-1 are primarily astrocytic, whereas EAAC1 and EAAT4 are neuronal. Using immunoblotting and immunohistochemistry with subtype-specific antipeptide antibodies, we examined the protein expression and regional and cellular localization of each glutamate transporter subtype in embryonic and postnatal rat CNS. Each transporter had a specific pattern of expression. GLAST immunoreactivity was low prenatally but became enriched in cerebellar Bergmann glia early postnatally and then was also present in forebrain later postnatally. The post-translational modification of GLAST was unique among the subtypes; glycosylated GLAST increased with maturation, whereas nonglycosylated protein decreased in abundance postnatally. GLT-1 was present in fetal brain and spinal cord, with expression progressively increasing to adult levels throughout the neuraxis by postnatal day 26. Transient expression of GLT-1 immunoreactivity along axonal pathways was observed prenatally, in contrast to the exclusive localization of GLT-1 to astrocytes in the adult CNS. EAAC1, localized to neurons, was enriched in forebrain, diencephalon, and hindbrain during prenatal and postnatal development. EAAC1 expression was greater in newborn brain compared with adult brain. EAAT4 had a region-specific distribution; EAAT4 was mainly in cerebellum, localized to Purkinje cells, with much lower levels in forebrain. EAAT4 levels increased in cerebellum with age. We conclude that during CNS development the expression of glutamate transporter subtypes is differentially regulated, regionally segregated, and coordinated.
Key words: brain development; excitatory amino acid; perinatal brain damage; corticogenesis; excitotoxicity; striatum
This article has been cited by other articles:
T. M. DeSilva, A. Y. Kabakov, P. E. Goldhoff, J. J. Volpe, and P. A. Rosenberg
Regulation of Glutamate Transport in Developing Rat Oligodendrocytes
J. Neurosci., June 17, 2009; 29(24): 7898 - 7908.
[Abstract] [Full Text] [PDF]
H. Nie and H.-R. Weng
Glutamate Transporters Prevent Excessive Activation of NMDA Receptors and Extrasynaptic Glutamate Spillover in the Spinal Dorsal Horn
J Neurophysiol, April 1, 2009; 101(4): 2041 - 2051.
[Abstract] [Full Text] [PDF]
C. Allene, A. Cattani, J. B. Ackman, P. Bonifazi, L. Aniksztejn, Y. Ben-Ari, and R. Cossart
Sequential Generation of Two Distinct Synapse-Driven Network Patterns in Developing Neocortex
J. Neurosci., November 26, 2008; 28(48): 12851 - 12863.
[Abstract] [Full Text] [PDF]
A. V. Jelenkovic, M. D. Jovanovic, D. D. Stanimirovic, D. D. Bokonjic, G. G. Ocic, and B. S. Boskovic
Beneficial Effects of Ceftriaxone Against Pentylenetetrazole-Evoked Convulsions
Experimental Biology and Medicine, November 1, 2008; 233(11): 1389 - 1394.
[Abstract] [Full Text] [PDF]
C. Takasaki, R. Okada, A. Mitani, M. Fukaya, M. Yamasaki, Y. Fujihara, T. Shirakawa, K. Tanaka, and M. Watanabe
Glutamate Transporters Regulate Lesion-Induced Plasticity in the Developing Somatosensory Cortex
J. Neurosci., May 7, 2008; 28(19): 4995 - 5006.
[Abstract] [Full Text] [PDF]
B. Murdoch and A. J. Roskams
A Novel Embryonic Nestin-Expressing Radial Glia-Like Progenitor Gives Rise to Zonally Restricted Olfactory and Vomeronasal Neurons
J. Neurosci., April 16, 2008; 28(16): 4271 - 4282.
[Abstract] [Full Text] [PDF]
A. A. Cattani, V. D. Bonfardin, A. Represa, Y. Ben-Ari, and L. Aniksztejn
Generation of Slow Network Oscillations in the Developing Rat Hippocampus After Blockade of Glutamate Uptake
J Neurophysiol, October 1, 2007; 98(4): 2324 - 2336.
[Abstract] [Full Text] [PDF]
M. R. Regan, Y. H. Huang, Y. S. Kim, M. I. Dykes-Hoberg, L. Jin, A. M. Watkins, D. E. Bergles, and J. D. Rothstein
Variations in Promoter Activity Reveal a Differential Expression and Physiology of Glutamate Transporters by Glia in the Developing and Mature CNS
J. Neurosci., June 20, 2007; 27(25): 6607 - 6619.
[Abstract] [Full Text] [PDF]
G. P. Schools, M. Zhou, and H. K. Kimelberg
Development of Gap Junctions in Hippocampal Astrocytes: Evidence That Whole Cell Electrophysiological Phenotype Is an Intrinsic Property of the Individual Cell
J Neurophysiol, September 1, 2006; 96(3): 1383 - 1392.
[Abstract] [Full Text] [PDF]
A. M. Feoli, I. Siqueira, L. M. V. Almeida, A. C. Tramontina, C. Battu, S. T. Wofchuk, C. Gottfried, M. L. Perry, and C.-A. Goncalves
Brain Glutathione Content and Glutamate Uptake Are Reduced in Rats Exposed to Pre- and Postnatal Protein Malnutrition
J. Nutr., September 1, 2006; 136(9): 2357 - 2361.
[Abstract] [Full Text] [PDF]
M. Zhou, G. P. Schools, and H. K. Kimelberg
Development of GLAST(+) Astrocytes and NG2(+) Glia in Rat Hippocampus CA1: Mature Astrocytes Are Electrophysiologically Passive
J Neurophysiol, January 1, 2006; 95(1): 134 - 143.
[Abstract] [Full Text] [PDF]
J. D. Dougherty, A. D. R. Garcia, I. Nakano, M. Livingstone, B. Norris, R. Polakiewicz, E. M. Wexler, M. V. Sofroniew, H. I. Kornblum, and D. H. Geschwind
PBK/TOPK, a Proliferating Neural Progenitor-Specific Mitogen-Activated Protein Kinase Kinase
J. Neurosci., November 16, 2005; 25(46): 10773 - 10785.
[Abstract] [Full Text] [PDF]
J. Zschocke, N. Bayatti, A. M. Clement, H. Witan, M. Figiel, J. Engele, and C. Behl
Differential Promotion of Glutamate Transporter Expression and Function by Glucocorticoids in Astrocytes from Various Brain Regions
J. Biol. Chem., October 14, 2005; 280(41): 34924 - 34932.
[Abstract] [Full Text] [PDF]
M. Hamann, D. J. Rossi, C. Mohr, A. L. Andrade, and D. Attwell
The electrical response of cerebellar Purkinje neurons to simulated ischaemia
Brain, October 1, 2005; 128(10): 2408 - 2420.
[Abstract] [Full Text] [PDF]
Y. Takayasu, M. Iino, W. Kakegawa, H. Maeno, K. Watase, K. Wada, D. Yanagihara, T. Miyazaki, O. Komine, M. Watanabe, et al.
Differential Roles of Glial and Neuronal Glutamate Transporters in Purkinje Cell Synapses
J. Neurosci., September 21, 2005; 25(38): 8788 - 8793.
[Abstract] [Full Text] [PDF]
J. S. Diamond
Deriving the Glutamate Clearance Time Course from Transporter Currents in CA1 Hippocampal Astrocytes: Transmitter Uptake Gets Faster during Development
J. Neurosci., March 16, 2005; 25(11): 2906 - 2916.
[Abstract] [Full Text] [PDF]
H.-X. Chen and S. N. Roper
Tonic Activity of Metabotropic Glutamate Receptors Is Involved in Developmental Modification of Short-Term Plasticity in the Neocortex
J Neurophysiol, August 1, 2004; 92(2): 838 - 844.
[Abstract] [Full Text] [PDF]
H. Huang and A. Bordey
Glial Glutamate Transporters Limit Spillover Activation of Presynaptic NMDA Receptors and Influence Synaptic Inhibition of Purkinje Neurons
J. Neurosci., June 23, 2004; 24(25): 5659 - 5669.
[Abstract] [Full Text] [PDF]
Y. H. Huang, S. R. Sinha, K. Tanaka, J. D. Rothstein, and D. E. Bergles
Astrocyte Glutamate Transporters Regulate Metabotropic Glutamate Receptor-Mediated Excitation of Hippocampal Interneurons
J. Neurosci., May 12, 2004; 24(19): 4551 - 4559.
[Abstract] [Full Text] [PDF]
G. Brasnjo and T. S. Otis
Isolation of glutamate transport-coupled charge flux and estimation of glutamate uptake at the climbing fiber-Purkinje cell synapse
PNAS, April 20, 2004; 101(16): 6273 - 6278.
[Abstract] [Full Text] [PDF]
M. Demarque, N. Villeneuve, J.-B. Manent, H. Becq, A. Represa, Y. Ben-Ari, and L. Aniksztejn
Glutamate Transporters Prevent the Generation of Seizures in the Developing Rat Neocortex
J. Neurosci., March 31, 2004; 24(13): 3289 - 3294.
[Abstract] [Full Text] [PDF]
T. Morita, K. Nakamura, M. Sawada, A. Shimada, K. Sato, H. Miyata, and E. Ohama
Inositol 1,4,5-Triphosphate Receptor Protein Immunohistochemistry of Cerebellar Purkinje Cells in Two Dogs with Hypoglycemia
Vet. Pathol., January 1, 2004; 41(1): 82 - 86.
[Abstract] [Full Text] [PDF]
Y. Wakisaka, A. Furuta, K. Masuda, W. Morikawa, M. Kuwano, and T. Iwaki
Cellular Distribution of NDRG1 Protein in the Rat Kidney and Brain During Normal Postnatal Development
J. Histochem. Cytochem., November 1, 2003; 51(11): 1515 - 1525.
[Abstract] [Full Text] [PDF]
B. Voutsinos-Porche, G. Knott, K. Tanaka, C. Quairiaux, E. Welker, and G. Bonvento
Glial Glutamate Transporters and Maturation of the Mouse Somatosensory Cortex
Cereb Cortex, October 1, 2003; 13(10): 1110 - 1121.
[Abstract] [Full Text] [PDF]
M. J. Palmer, H. Taschenberger, C. Hull, L. Tremere, and H. von Gersdorff
Synaptic Activation of Presynaptic Glutamate Transporter Currents in Nerve Terminals
J. Neurosci., June 15, 2003; 23(12): 4831 - 4841.
[Abstract] [Full Text] [PDF]
M. Tanaka, N. Maeda, M. Noda, and T. Marunouchi
A Chondroitin Sulfate Proteoglycan PTPzeta /RPTPbeta Regulates the Morphogenesis of Purkinje Cell Dendrites in the Developing Cerebellum
J. Neurosci., April 1, 2003; 23(7): 2804 - 2814.
[Abstract] [Full Text] [PDF]
C. A. G. Marshall and J. E. Goldman
Subpallial Dlx2-Expressing Cells Give Rise to Astrocytes and Oligodendrocytes in the Cerebral Cortex and White Matter
J. Neurosci., November 15, 2002; 22(22): 9821 - 9830.
[Abstract] [Full Text] [PDF]
L. Barakat and A. Bordey
GAT-1 and Reversible GABA Transport in Bergmann Glia in Slices
J Neurophysiol, September 1, 2002; 88(3): 1407 - 1419.
[Abstract] [Full Text] [PDF]
Y. Ikegaya, S. Matsuura, S. Ueno, A. Baba, M. K. Yamada, N. Nishiyama, and N. Matsuki
beta -Amyloid Enhances Glial Glutamate Uptake Activity and Attenuates Synaptic Efficacy
J. Biol. Chem., August 23, 2002; 277(35): 32180 - 32186.
[Abstract] [Full Text] [PDF]
J.M. Soria and M. Valdeolmillos
Receptor-activated Calcium Signals in Tangentially Migrating Cortical Cells
Cereb Cortex, August 1, 2002; 12(8): 831 - 839.
[Abstract] [Full Text] [PDF]
C.-S. Yuan, L. Dey, J.-T. Xie, and H. H. Aung
Pharmacology and toxicology of astrocyte-neuron glutamate transport and cycling.
J. Pharmacol. Exp. Ther., April 1, 2002; 301(1): 1 - 6.
[Abstract] [Full Text] [PDF]
W. Chen, C. Aoki, V. Mahadomrongkul, C. E. Gruber, G. J. Wang, R. Blitzblau, N. Irwin, and P. A. Rosenberg
Expression of a Variant Form of the Glutamate Transporter GLT1 in Neuronal Cultures and in Neurons and Astrocytes in the Rat Brain
J. Neurosci., March 15, 2002; 22(6): 2142 - 2152.
[Abstract] [Full Text] [PDF]
K. Suzuki, Y. Ikegaya, S. Matsuura, Y. Kanai, H. Endou, and N. Matsuki
Transient upregulation of the glial glutamate transporter GLAST in response to fibroblast growth factor, insulin-like growth factor and epidermal growth factor in cultured astrocytes
J. Cell Sci., March 12, 2002; 114(20): 3717 - 3725.
[Abstract] [Full Text] [PDF]
E. A. Proper, G. Hoogland, S. M. Kappen, G. H. Jansen, M. G. A. Rensen, L. H. Schrama, C. W. M. van Veelen, P. C. van Rijen, O. van Nieuwenhuizen, W. H. Gispen, et al.
Distribution of glutamate transporters in the hippocampus of patients with pharmaco-resistant temporal lobe epilepsy
Brain, January 1, 2002; 125(1): 32 - 43.
[Abstract] [Full Text] [PDF]
M. Zhou and H. K. Kimelberg
Freshly Isolated Hippocampal CA1 Astrocytes Comprise Two Populations Differing in Glutamate Transporter and AMPA Receptor Expression
J. Neurosci., October 15, 2001; 21(20): 7901 - 7908.
[Abstract] [Full Text] [PDF]
R. E. Smith, V. Haroutunian, K. L. Davis, and J. H. Meador-Woodruff
Expression of Excitatory Amino Acid Transporter Transcripts in the Thalamus of Subjects With Schizophrenia
Am J Psychiatry, September 1, 2001; 158(9): 1393 - 1399.
[Abstract] [Full Text] [PDF]
C. K. Vorwerk, R. Naskar, F. Schuettauf, K. Quinto, D. Zurakowski, G. Gochenauer, M. B. Robinson, S. A. Mackler, and E. B. Dreyer
Depression of Retinal Glutamate Transporter Function Leads to Elevated Intravitreal Glutamate Levels and Ganglion Cell Death
Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3615 - 3621.
[Abstract] [Full Text]
C. Metin, J.-P. Denizot, and N. Ropert
Intermediate Zone Cells Express Calcium-Permeable AMPA Receptors and Establish Close Contact with Growing Axons
J. Neurosci., January 15, 2000; 20(2): 696 - 708.
[Abstract] [Full Text] [PDF]
S. Kojima, T. Nakamura, T. Nidaira, K. Nakamura, N. Ooashi, E. Ito, K. Watase, K. Tanaka, K. Wada, Y. Kudo, et al.
Optical Detection of Synaptically Induced Glutamate Transport in Hippocampal Slices
J. Neurosci., April 1, 1999; 19(7): 2580 - 2588.
[Abstract] [Full Text] [PDF]
K. P. Lehre and N. C. Danbolt
The Number of Glutamate Transporter Subtype Molecules at Glutamatergic Synapses: Chemical and Stereological Quantification in Young Adult Rat Brain
J. Neurosci., November 1, 1998; 18(21): 8751 - 8757.
[Abstract] [Full Text] [PDF]
D. E. Bergles and C. E. Jahr
Glial Contribution to Glutamate Uptake at Schaffer Collateral-Commissural Synapses in the Hippocampus
J. Neurosci., October 1, 1998; 18(19): 7709 - 7716.
[Abstract] [Full Text] [PDF]
K. Yamada, M. Watanabe, T. Shibata, M. Nagashima, K. Tanaka, and Y. Inoue
Glutamate Transporter GLT-1 Is Transiently Localized on Growing Axons of the Mouse Spinal Cord before Establishing Astrocytic Expression
J. Neurosci., August 1, 1998; 18(15): 5706 - 5713.
[Abstract] [Full Text] [PDF]
S. Mennerick, R. P. Dhond, A. Benz, W. Xu, J. D. Rothstein, N. C. Danbolt, K. E. Isenberg, and C. F. Zorumski
Neuronal Expression of the Glutamate Transporter GLT-1 in Hippocampal Microcultures
J. Neurosci., June 15, 1998; 18(12): 4490 - 4499.
[Abstract] [Full Text] [PDF]
B. D. Schlag, J. R. Vondrasek, M. Munir, A. Kalandadze, O. A. Zelenaia, J. D. Rothstein, and M. B. Robinson
Regulation of the Glial Na+-Dependent Glutamate Transporters by Cyclic AMP Analogs and Neurons
Mol. Pharmacol., March 1, 1998; 53(3): 355 - 369.
[Abstract] [Full Text]
S. Li, G. A. R. Mealing, P. Morley, and P. K. Stys
Novel Injury Mechanism in Anoxia and Trauma of Spinal Cord White Matter: Glutamate Release via Reverse Na+-dependent Glutamate Transport
J. Neurosci., July 15, 1999; 19(14): RC16 - RC16.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|