 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
Volume 17, Number 22, Issue of November 15, 1997 pp. 8687-8694
The Mechanism of cAMP-Mediated Enhancement at a Cerebellar Synapse
Received June 2, 1997; revised Aug. 6, 1997; accepted Aug. 29, 1997.
Chinfei Chen and Wade G. Regehr Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115
Increases in cAMP have been shown previously to enhance the strength of the granule cell to Purkinje cell synapse. We have examined the mechanisms underlying this enhancement in rat cerebellar brain slices. Elevation of cAMP levels by forskolin increased synaptic currents in a dose-dependent manner. Fluorometric calcium measurements revealed that forskolin did not affect presynaptic calcium influx or resting calcium levels. The waveform of the presynaptic volley was also unaltered, indicating that changes in the presynaptic action potential did not contribute to synaptic enhancement. However, forskolin enhanced the frequency but not the size of spontaneous miniature EPSCs. There was a one-to-one correspondence between increases of spontaneous and evoked neurotransmitter release. These results suggest that forskolin increases release at this synapse via presynaptic mechanisms that do not alter calcium influx. The effect of forskolin on paired-pulse facilitation was examined to assess the relative contributions of changes in the probability of release (p) and changes in the number of functional release sites (n) to this form of enhancement. These experiments suggest that although small changes in n cannot be excluded, most of the enhancement arises from increases in p.
Key words: synaptic modulation; cAMP; magnesium green; fura-2; mEPSC; forskolin; Purkinje cell; granule cell; paired-pulse facilitation
This article has been cited by other articles:
V. A. Bender, J. R. Pugh, and C. E. Jahr
Presynaptically Expressed Long-Term Potentiation Increases Multivesicular Release at Parallel Fiber Synapses
J. Neurosci., September 2, 2009; 29(35): 10974 - 10978.
[Abstract] [Full Text] [PDF]
B. Pan, C. J. Hillard, and Q.-s. Liu
D2 Dopamine Receptor Activation Facilitates Endocannabinoid-Mediated Long-Term Synaptic Depression of GABAergic Synaptic Transmission in Midbrain Dopamine Neurons via cAMP-Protein Kinase A Signaling
J. Neurosci., December 24, 2008; 28(52): 14018 - 14030.
[Abstract] [Full Text] [PDF]
T. J. Searl and E. M. Silinsky
Mechanisms of Neuromodulation as Dissected Using Sr2+ at Motor Nerve Endings
J Neurophysiol, June 1, 2008; 99(6): 2779 - 2788.
[Abstract] [Full Text] [PDF]
E. M. Krauter, D. R. Linden, K. A. Sharkey, and G. M. Mawe
Synaptic plasticity in myenteric neurons of the guinea-pig distal colon: presynaptic mechanisms of inflammation-induced synaptic facilitation
J. Physiol., June 1, 2007; 581(2): 787 - 800.
[Abstract] [Full Text] [PDF]
D.-l. Qiu and T. Knopfel
An NMDA Receptor/Nitric Oxide Cascade in Presynaptic Parallel Fiber-Purkinje Neuron Long-Term Potentiation
J. Neurosci., March 28, 2007; 27(13): 3408 - 3415.
[Abstract] [Full Text] [PDF]
C. O. Alberto, R. B. Trask, M. E. Quinlan, and M. Hirasawa
Bidirectional Dopaminergic Modulation of Excitatory Synaptic Transmission in Orexin Neurons
J. Neurosci., September 27, 2006; 26(39): 10043 - 10050.
[Abstract] [Full Text] [PDF]
B. J. van Beugen, R. Y. Nagaraja, and C. Hansel
Climbing fiber-evoked endocannabinoid signaling heterosynaptically suppresses presynaptic cerebellar long-term potentiation.
J. Neurosci., August 9, 2006; 26(32): 8289 - 8294.
[Abstract] [Full Text] [PDF]
R. Tonini, S. Ciardo, M. Cerovic, T. Rubino, D. Parolaro, M. Mazzanti, and R. Zippel
ERK-Dependent Modulation of Cerebellar Synaptic Plasticity after Chronic {Delta}9-Tetrahydrocannabinol Exposure
J. Neurosci., May 24, 2006; 26(21): 5810 - 5818.
[Abstract] [Full Text] [PDF]
C.-C. Huang and K.-S. Hsu
Presynaptic Mechanism Underlying cAMP-Induced Synaptic Potentiation in Medial Prefrontal Cortex Pyramidal Neurons
Mol. Pharmacol., March 1, 2006; 69(3): 846 - 856.
[Abstract] [Full Text] [PDF]
N. K. Charlie, M. A. Schade, A. M. Thomure, and K. G. Miller
Presynaptic UNC-31 (CAPS) Is Required to Activate the G{alpha}s Pathway of the Caenorhabditis elegans Synaptic Signaling Network
Genetics, February 1, 2006; 172(2): 943 - 961.
[Abstract] [Full Text] [PDF]
M. Yamasaki, K. Hashimoto, and M. Kano
Miniature Synaptic Events Elicited by Presynaptic Ca2+ Rise Are Selectively Suppressed by Cannabinoid Receptor Activation in Cerebellar Purkinje Cells
J. Neurosci., January 4, 2006; 26(1): 86 - 95.
[Abstract] [Full Text] [PDF]
W. Kakegawa and M. Yuzaki
From The Cover: A mechanism underlying AMPA receptor trafficking during cerebellar long-term potentiation
PNAS, December 6, 2005; 102(49): 17846 - 17851.
[Abstract] [Full Text] [PDF]
A. V. Derbenev, C. L. Linn, and P. S. Guth
Muscarinic ACh Receptor Activation Causes Transmitter Release From Isolated Frog Vestibular Hair Cells
J Neurophysiol, November 1, 2005; 94(5): 3134 - 3142.
[Abstract] [Full Text] [PDF]
S. L. Smith and T. S. Otis
Pattern-dependent, simultaneous plasticity differentially transforms the input-output relationship of a feedforward circuit
PNAS, October 11, 2005; 102(41): 14901 - 14906.
[Abstract] [Full Text] [PDF]
S. Seino and T. Shibasaki
PKA-Dependent and PKA-Independent Pathways for cAMP-Regulated Exocytosis
Physiol Rev, October 1, 2005; 85(4): 1303 - 1342.
[Abstract] [Full Text] [PDF]
M. A. Schade, N. K. Reynolds, C. M. Dollins, and K. G. Miller
Mutations That Rescue the Paralysis of Caenorhabditis elegans ric-8 (Synembryn) Mutants Activate the G{alpha}s Pathway and Define a Third Major Branch of the Synaptic Signaling Network
Genetics, February 1, 2005; 169(2): 631 - 649.
[Abstract] [Full Text] [PDF]
N. K. Reynolds, M. A. Schade, and K. G. Miller
Convergent, RIC-8-Dependent G{alpha} Signaling Pathways in the Caenorhabditis elegans Synaptic Signaling Network
Genetics, February 1, 2005; 169(2): 651 - 670.
[Abstract] [Full Text] [PDF]
N. Zhong and R. S. Zucker
cAMP Acts on Exchange Protein Activated by cAMP/cAMP-Regulated Guanine Nucleotide Exchange Protein to Regulate Transmitter Release at the Crayfish Neuromuscular Junction
J. Neurosci., January 5, 2005; 25(1): 208 - 214.
[Abstract] [Full Text] [PDF]
S. P. Brown, P. K. Safo, and W. G. Regehr
Endocannabinoids Inhibit Transmission at Granule Cell to Purkinje Cell Synapses by Modulating Three Types of Presynaptic Calcium Channels
J. Neurosci., June 16, 2004; 24(24): 5623 - 5631.
[Abstract] [Full Text] [PDF]
M. Kaneko and T. Takahashi
Presynaptic Mechanism Underlying cAMP-Dependent Synaptic Potentiation
J. Neurosci., June 2, 2004; 24(22): 5202 - 5208.
[Abstract] [Full Text] [PDF]
H. Daniel, A. Rancillac, and F. Crepel
Mechanisms underlying cannabinoid inhibition of presynaptic Ca2+ influx at parallel fibre synapses of the rat cerebellum
J. Physiol., May 15, 2004; 557(1): 159 - 174.
[Abstract] [Full Text] [PDF]
J. E. Lewis and L. Maler
Synaptic Dynamics on Different Time Scales in a Parallel Fiber Feedback Pathway of the Weakly Electric Fish
J Neurophysiol, February 1, 2004; 91(2): 1064 - 1070.
[Abstract] [Full Text] [PDF]
V. Lev-Ram, S. B. Mehta, D. Kleinfeld, and R. Y. Tsien
Reversing cerebellar long-term depression
PNAS, December 23, 2003; 100(26): 15989 - 15993.
[Abstract] [Full Text] [PDF]
M. Hirasawa and Q. J. Pittman
From the Cover: Nifedipine facilitates neurotransmitter release independently of calcium channels
PNAS, May 13, 2003; 100(10): 6139 - 6144.
[Abstract] [Full Text] [PDF]
R. B. Renden and K. Broadie
Mutation and Activation of Galpha s Similarly Alters Pre- and Postsynaptic Mechanisms Modulating Neurotransmission
J Neurophysiol, May 1, 2003; 89(5): 2620 - 2638.
[Abstract] [Full Text] [PDF]
H. Kamiya, K. Umeda, S. Ozawa, and T. Manabe
Presynaptic Ca2+ Entry Is Unchanged during Hippocampal Mossy Fiber Long-Term Potentiation
J. Neurosci., December 15, 2002; 22(24): 10524 - 10528.
[Abstract] [Full Text] [PDF]
J. M. Brundege and J. T. Williams
Differential Modulation of Nucleus Accumbens Synapses
J Neurophysiol, July 1, 2002; 88(1): 142 - 151.
[Abstract] [Full Text] [PDF]
V. Lev-Ram, S. T. Wong, D. R. Storm, and R. Y. Tsien
A new form of cerebellar long-term potentiation is postsynaptic and depends on nitric oxide but not cAMP
PNAS, June 11, 2002; 99(12): 8389 - 8393.
[Abstract] [Full Text] [PDF]
I. Slutsky, G. Rashkovan, H. Parnas, and I. Parnas
Ca2+-Independent Feedback Inhibition of Acetylcholine Release in Frog Neuromuscular Junction
J. Neurosci., May 1, 2002; 22(9): 3426 - 3433.
[Abstract] [Full Text] [PDF]
C.-C. Huang, Y.-L. Chen, S.-W. Lo, and K.-S. Hsu
Activation of cAMP-Dependent Protein Kinase Suppresses the Presynaptic Cannabinoid Inhibition of Glutamatergic Transmission at Corticostriatal Synapses
Mol. Pharmacol., March 1, 2002; 61(3): 578 - 585.
[Abstract] [Full Text] [PDF]
A. R. Carter, C. Chen, P. M. Schwartz, and R. A. Segal
Brain-Derived Neurotrophic Factor Modulates Cerebellar Plasticity and Synaptic Ultrastructure
J. Neurosci., February 15, 2002; 22(4): 1316 - 1327.
[Abstract] [Full Text] [PDF]
J. Mellor, R. A. Nicoll, and D. Schmitz
Mediation of Hippocampal Mossy Fiber Long-Term Potentiation by Presynaptic Ih Channels
Science, January 4, 2002; 295(5552): 143 - 147.
[Abstract] [Full Text] [PDF]
J. N. Jovanovic, T. S. Sihra, A. C. Nairn, H. C. Hemmings Jr, P. Greengard, and A. J. Czernik
Opposing Changes in Phosphorylation of Specific Sites in Synapsin I During Ca2+-Dependent Glutamate Release in Isolated Nerve Terminals
J. Neurosci., October 15, 2001; 21(20): 7944 - 7953.
[Abstract] [Full Text] [PDF]
S. Jacoby, R. E Sims, and N. A Hartell
Nitric oxide is required for the induction and heterosynaptic spread of long-term potentiation in rat cerebellar slices
J. Physiol., September 15, 2001; 535(3): 825 - 839.
[Abstract] [Full Text] [PDF]
C. J. Price and Q. J. Pittman
Dopamine D4 Receptor Activation Inhibits Presynaptically Glutamatergic Neurotransmission in the Rat Supraoptic Nucleus
J Neurophysiol, September 1, 2001; 86(3): 1149 - 1155.
[Abstract] [Full Text] [PDF]
M. Ito
Cerebellar Long-Term Depression: Characterization, Signal Transduction, and Functional Roles
Physiol Rev, July 1, 2001; 81(3): 1143 - 1195.
[Abstract] [Full Text] [PDF]
W. J. Wolfgang, A. Hoskote, I. J. H. Roberts, S. Jackson, and M. Forte
Genetic Analysis of the Drosophila Gs{{alpha}} Gene
Genetics, July 1, 2001; 158(3): 1189 - 1201.
[Abstract] [Full Text] [PDF]
J. W. Barclay and R. M. Robertson
Enhancement of Short-Term Synaptic Plasticity by Prior Environmental Stress
J Neurophysiol, March 1, 2001; 85(3): 1332 - 1335.
[Abstract] [Full Text] [PDF]
S. Hilfiker, A. J Czernik, P. Greengard, and G. J Augustine
Tonically active protein kinase A regulates neurotransmitter release at the squid giant synapse
J. Physiol., February 15, 2001; 531(1): 141 - 146.
[Abstract] [Full Text] [PDF]
D. I. Evans, R. S. G. Jones, and G. Woodhall
Differential Actions of PKA and PKC in the Regulation of Glutamate Release by Group III mGluRs in the Entorhinal Cortex
J Neurophysiol, February 1, 2001; 85(2): 571 - 579.
[Abstract] [Full Text] [PDF]
J. S. Richards
New Signaling Pathways for Hormones and Cyclic Adenosine 3',5'-Monophosphate Action in Endocrine Cells
Mol. Endocrinol., February 1, 2001; 15(2): 209 - 218.
[Abstract] [Full Text]
S. Katsurabayashi, H. Kubota, Z. M. Wang, J. S. Rhee, and N. Akaike
cAMP-Dependent Presynaptic Regulation of Spontaneous Glycinergic IPSCs in Mechanically Dissociated Rat Spinal Cord Neurons
J Neurophysiol, January 1, 2001; 85(1): 332 - 340.
[Abstract] [Full Text] [PDF]
J. T. Williams, M. J. Christie, and O. Manzoni
Cellular and Synaptic Adaptations Mediating Opioid Dependence
Physiol Rev, January 1, 2001; 81(1): 299 - 343.
[Abstract] [Full Text] [PDF]
T. Sakaba and E. Neher
Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held
PNAS, December 22, 2000; (2000) 21541098.
[Abstract] [Full Text]
F. Saitow, S.'I. Satake, J. Yamada, and S. Konishi
beta -Adrenergic Receptor-Mediated Presynaptic Facilitation of Inhibitory GABAergic Transmission at Cerebellar Interneuron-Purkinje Cell Synapses
J Neurophysiol, October 1, 2000; 84(4): 2016 - 2025.
[Abstract] [Full Text] [PDF]
K. A. Takahashi and D. J. Linden
Cannabinoid Receptor Modulation of Synapses Received by Cerebellar Purkinje Cells
J Neurophysiol, March 1, 2000; 83(3): 1167 - 1180.
[Abstract] [Full Text] [PDF]
C. C. Gandhi and L. D. Matzel
Modulation of Presynaptic Action Potential Kinetics Underlies Synaptic Facilitation of Type B Photoreceptors after Associative Conditioning in Hermissenda
J. Neurosci., March 1, 2000; 20(5): 2022 - 2035.
[Abstract] [Full Text] [PDF]
D. J. Linden and S. Ahn
Activation of Presynaptic cAMP-Dependent Protein Kinase Is Required for Induction of Cerebellar Long-Term Potentiation
J. Neurosci., December 1, 1999; 19(23): 10221 - 10227.
[Abstract] [Full Text] [PDF]
S. Koyama, C. Kubo, J.-S. Rhee, and N. Akaike
Presynaptic serotonergic inhibition of GABAergic synaptic transmission in mechanically dissociated rat basolateral amygdala neurons
J. Physiol., July 15, 1999; 518(2): 525 - 538.
[Abstract] [Full Text] [PDF]
M. Yoshihara, A. Ueda, D. Zhang, D. L. Deitcher, T. L. Schwarz, and Y. Kidokoro
Selective Effects of neuronal-synaptobrevin Mutations on Transmitter Release Evoked by Sustained Versus Transient Ca2+ Increases and by cAMP
J. Neurosci., April 1, 1999; 19(7): 2432 - 2441.
[Abstract] [Full Text] [PDF]
Y. Shoji, J. Delfs, and J. T. Williams
Presynaptic Inhibition of GABAB-Mediated Synaptic Potentials in the Ventral Tegmental Area during Morphine Withdrawal
J. Neurosci., March 15, 1999; 19(6): 2347 - 2355.
[Abstract] [Full Text] [PDF]
S.-J. Wang, L.-L. Cheng, and P.-W. Gean
Cross-Modulation of Synaptic Plasticity by beta -Adrenergic and 5-HT1A Receptors in the Rat Basolateral Amygdala
J. Neurosci., January 15, 1999; 19(2): 570 - 577.
[Abstract] [Full Text] [PDF]
P. P. Atluri and W. G. Regehr
Delayed Release of Neurotransmitter from Cerebellar Granule Cells
J. Neurosci., October 15, 1998; 18(20): 8214 - 8227.
[Abstract] [Full Text] [PDF]
B. Chieng and J. T. Williams
Increased Opioid Inhibition of GABA Release in Nucleus Accumbens during Morphine Withdrawal
J. Neurosci., September 1, 1998; 18(17): 7033 - 7039.
[Abstract] [Full Text] [PDF]
T. Sakaba and E. Neher
Preferential potentiation of fast-releasing synaptic vesicles by cAMP at the calyx of Held
PNAS, January 2, 2001; 98(1): 331 - 336.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|