QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (51) Citing Articles via Google Scholar Google Scholar Articles by Pozzo-Miller, L. D. Articles by Andrews, S. B. Search for Related Content PubMed PubMed Citation Articles by Pozzo-Miller, L. D. Articles by Andrews, S. B.

 Previous Article  |  Next Article 

Volume 17, Number 22, Issue of November 15, 1997 pp. 8729-8738

Activity-Dependent Calcium Sequestration in Dendrites of Hippocampal Neurons in Brain Slices

Received June 3, 1997; revised Aug. 29, 1997; accepted Sept. 8, 1997.

Lucas D. Pozzo-Miller^{1, 2}, Natalia B. Pivovarova¹, Richard D. Leapman³, Roger A. Buchanan¹, Thomas S. Reese^{1, 2}, and S. Brian Andrews^{1, 2}

¹ Laboratory of Neurobiology, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, ² Marine Biological Laboratory, Woods Hole, Massachusetts 02543, and ³ Biomedical Engineering and Instrumentation Program, National Center for Research Resources, National Institutes of Health, Bethesda, Maryland 20892

Synaptic activity-dependent changes in the spatio-temporal distribution of calcium ions regulate important neuronal functions such as dendritic integration and synaptic plasticity, but the processes that terminate the free Ca²⁺ transients associated with these changes remain unclear. We have characterized at the electron microscopic level the intracellular compartments involved in buffering free Ca²⁺ transients in dendritic cytoplasm of CA3 neurons by measuring the larger changes in the concentrations of total Ca that persist for several minutes after neuronal activity. Quantitative energy-dispersive x-ray microanalysis of cryosections from hippocampal slice cultures rapidly frozen 3 min after afferent synaptic activity identified a subset of dendritic endoplasmic reticulum (ER) as a high-capacity Ca²⁺ buffer. Calcium sequestration by cisterns of this subset of ER was graded, reversible, and dependent on a thapsigargin-sensitive Ca²⁺-ATPase. Sequestration was so robust that after repetitive high-frequency stimulation the Ca content of responsive ER cisterns increased as much as 20-fold. These results demonstrate that a subpopulation of ER is the major dendritic Ca sequestration compartment in the minutes after neuronal activity.

Key words: calcium regulation; calcium sequestration; hippocampus; CA3; dendrites; endoplasmic reticulum; synaptic activity; hippocampal slice cultures; X-ray microanalysis

This article has been cited by other articles:

				M. Patterson, J. Sneyd, and D. D. Friel Depolarization-induced Calcium Responses in Sympathetic Neurons: Relative Contributions from Ca2+ Entry, Extrusion, ER/Mitochondrial Ca2+ Uptake and Release, and Ca2+ Buffering J. Gen. Physiol., January 1, 2007; 129(1): 29 - 56. [Abstract] [Full Text] [PDF]

				B. Zuber, I. Nikonenko, P. Klauser, D. Muller, and J. Dubochet The mammalian central nervous synaptic cleft contains a high density of periodically organized complexes PNAS, December 27, 2005; 102(52): 19192 - 19197. [Abstract] [Full Text] [PDF]

				J. M. Power and P. Sah Intracellular calcium store filling by an L-type calcium current in the basolateral amygdala at subthreshold membrane potentials J. Physiol., January 15, 2005; 562(2): 439 - 453. [Abstract] [Full Text] [PDF]

				A. Verkhratsky Physiology and Pathophysiology of the Calcium Store in the Endoplasmic Reticulum of Neurons Physiol Rev, January 1, 2005; 85(1): 201 - 279. [Abstract] [Full Text] [PDF]

				N. B. Pivovarova, H. V. Nguyen, C. A. Winters, C. A. Brantner, C. L. Smith, and S. B. Andrews Excitotoxic Calcium Overload in a Subpopulation of Mitochondria Triggers Delayed Death in Hippocampal Neurons J. Neurosci., June 16, 2004; 24(24): 5611 - 5622. [Abstract] [Full Text] [PDF]

				N. B. Pivovarova, L. D. Pozzo-Miller, J. Hongpaisan, and S. B. Andrews Correlated Calcium Uptake and Release by Mitochondria and Endoplasmic Reticulum of CA3 Hippocampal Dendrites after Afferent Synaptic Stimulation J. Neurosci., December 15, 2002; 22(24): 10653 - 10661. [Abstract] [Full Text] [PDF]

				J. Meldolesi Rapidly Exchanging Ca2+ Stores: Ubiquitous Partners of Surface Channels in Neurons Physiology, August 1, 2002; 17(4): 144 - 149. [Abstract] [Full Text] [PDF]

				V. Durr and M. Egelhaaf In Vivo Calcium Accumulation in Presynaptic and Postsynaptic Dendrites of Visual Interneurons J Neurophysiol, December 1, 1999; 82(6): 3327 - 3338. [Abstract] [Full Text] [PDF]

				S. Koizumi, P. Lipp, M. J. Berridge, and M. D. Bootman Regulation of Ryanodine Receptor Opening by Lumenal Ca2+ Underlies Quantal Ca2+ Release in PC12 Cells J. Biol. Chem., November 19, 1999; 274(47): 33327 - 33333. [Abstract] [Full Text] [PDF]

				N. B. Pivovarova, J. Hongpaisan, S. B. Andrews, and D. D. Friel Depolarization-Induced Mitochondrial Ca Accumulation in Sympathetic Neurons: Spatial and Temporal Characteristics J. Neurosci., August 1, 1999; 19(15): 6372 - 6384. [Abstract] [Full Text] [PDF]

				L. D. Pozzo-Miller, T. Inoue, and D. D. Murphy Estradiol Increases Spine Density and NMDA-Dependent Ca2+ Transients in Spines of CA1 Pyramidal Neurons From Hippocampal Slices J Neurophysiol, March 1, 1999; 81(3): 1404 - 1411. [Abstract] [Full Text] [PDF]

				A. Gardiol, C. Racca, and A. Triller Dendritic and Postsynaptic Protein Synthetic Machinery J. Neurosci., January 1, 1999; 19(1): 168 - 179. [Abstract] [Full Text] [PDF]

				J. Meldolesi and T. Pozzan The Heterogeneity of ER Ca2+ Stores Has a Key Role in Nonmuscle Cell Signaling and Function J. Cell Biol., September 21, 1998; 142(6): 1395 - 1398. [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help