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Volume 17, Number 24, Issue of December 15, 1997 pp. 9565-9572

Isoform Specificity in the Relationship of Actin to Dendritic Spines

Received July 28, 1997; revised Sept. 29, 1997; accepted Oct. 3, 1997.

Stefanie Kaech, Maria Fischer, Thierry Doll, and Andrew Matus

Friedrich Miescher Institute, 4002 Basel, Switzerland

Dendritic spines contain high concentrations of actin, but neither the isoforms involved nor the mechanism of accumulation is known. In situ hybridization with specific probes established that beta - and gamma -cytoplasmic actins are selectively expressed at high levels by spine-bearing neurons. Transfecting cultured hippocampal neurons with epitope-tagged actin isoforms showed that cytoplasmic beta - and gamma -cytoplasmic actins are correctly targeted to spines, whereas alpha -cardiac muscle actin, which is normally absent from neurons, formed aggregates in dendrites. The transfected actin cDNAs contained only coding domains, suggesting that spine targeting involves amino acid sequences in the proteins, an interpretation supported by experiments with chimeric cDNAs in which C-terminal actin sequences were found to be determinative in spine targeting. By contrast to actin, microtubule components, including tubulin and MAP2, were restricted to the dendritic shaft domain. The close association of cytoplasmic actins with spines together with their general involvement in cell surface motility further supports the idea that actin motility-based changes in spine shape may contribute to synaptic plasticity.

Key words: cytoskeleton; dendrites; synapses; neuroanatomy; brain; central nervous system; microtubules; microtubule-associated proteins; MAP2; gene expression; plasticity




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