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Volume 17, Number 3, Issue of February 1, 1997 pp. 917-923
Copyright ©1997 Society for Neuroscience

Visual Pigment Assignments in Regenerated Retina

Received Aug. 13, 1996; revised Oct. 10, 1996; accepted Nov. 11, 1996.

David A. Cameron, M. Carter Cornwall, and Edward F. MacNichol Jr.

Department of Physiology, Boston University School of Medicine, Boston, Massachusetts 02118

Retinas of adult teleost fish can regenerate after injury. Two important issues regarding this phenomenon are the assembly of the regenerated retina and the neuronal images of the visual scene that the regenerated retina produces. Here we report experiments in which the visual pigment content of photoreceptors derived from native and regenerated sunfish retinas was determined by microspectrophotometry. In native retina, there is an apparently perfect correspondence between cone morphology and visual pigment content; all rods contain a middle-wavelength pigment, all single cones contain a different middle-wavelength pigment, and all double cone members contain a long-wavelength pigment. The visual pigments in regenerated rods and double cones were the same as in native retina; however, triple cones, a morphology never observed in native retina, contained the long-wavelength pigment. Moreover, although ~60% of regenerated single cones contained the expected middle-wavelength pigment, all other single cones contained the long-wavelength pigment. This mismatch between morphology of regenerated single cones and their visual pigment assignment indicated the following: (1) There is a degree of independence between the mechanisms that establish cone morphology and pigment content during regeneration, which suggests that cone photoreceptor regeneration is not a straightforward recapitulation of the normal cone photoreceptor developmental plan. (2) Although anomalous, the long-wavelength single cones may enable regenerated retina to restore the native spectral sampling of the visual scene.

Key words: retina; regeneration; photoreceptors; visual pigments; microspectrophotometry; rods; cones

This article has been cited by other articles:

				M. M. Mader and D. A. Cameron Photoreceptor Differentiation during Retinal Development, Growth, and Regeneration in a Metamorphic Vertebrate J. Neurosci., December 15, 2004; 24(50): 11463 - 11472. [Abstract] [Full Text] [PDF]

				J. Lem, N. V. Krasnoperova, P. D. Calvert, B. Kosaras, D. A. Cameron, M. Nicolo, C. L. Makino, and R. L. Sidman Morphological, physiological, and biochemical changes in rhodopsin knockout mice PNAS, January 19, 1999; 96(2): 736 - 741. [Abstract] [Full Text] [PDF]

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