QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (42) Citing Articles via Google Scholar Google Scholar Articles by Berton, F. Articles by Marquèze, B. Search for Related Content PubMed PubMed Citation Articles by Berton, F. Articles by Marquèze, B.

 Previous Article  |  Next Article 

Volume 17, Number 4, Issue of February 15, 1997 pp. 1206-1216
Copyright ©1997 Society for Neuroscience

Developmental Regulation of Synaptotagmin I, II, III, and IV mRNAs in the Rat CNS

Received July 29, 1996; revised Nov. 14, 1996; accepted Nov. 22, 1996.

Frédérique Berton, Cécile Iborra, Jeanne-Andrée Boudier, Michael J. Seagar, and Béatrice Marquèze

Institut National de la Santé et de la Recherche Médicale U 374, Institut Jean Roche, Faculté de Médecine-Secteur Nord, 13916 Marseille Cedex 20, France

Synaptotagmin I is an abundant synaptic vesicle protein that has an essential function in mediating Ca²⁺-triggered neurotransmitter release. We have analyzed the distribution of four neural synaptotagmin isoforms during postnatal development of the rat CNS by in situ hybridization. Synaptotagmin I, II, III, and IV genes have distinct patterns of spatiotemporal expression except in cerebellum granule cells, where the four transcripts were detected during the formation of parallel fiber/Purkinje cell synapses. Throughout development synaptotagmin I mRNAs were widely expressed in brain, whereas synaptotagmin II transcripts were predominant in spinal cord. At all stages synaptotagmin III mRNAs were expressed uniformly in most neurons examined, although at a low level. Synaptotagmin I, II, and III gene expressions mainly increased during development and persisted in adulthood, mirroring neuronal differentiation. Conversely, synaptotagmin IV transcripts were predominant during perinatal development in a heterogeneous population of neurons and subsequently were expressed uniformly at a low level. Intense labeling was observed in the hippocampal CA3 field and in the subiculum, but not in the CA1 field, of the newborn rat. In cerebral cortex, lamina-specific labeling was detected with a high expression in cell layer V. Only a small number of Purkinje cell clusters were labeled in the flocculus and paraflocculus of the cerebellum. Heterogeneous sets of neurons expressing synaptotagmin IV gene also were observed in spinal cord. We thus speculate that synaptotagmin IV may a play a role in the development of the mammalian nervous system.

Key words: synaptotagmin; exocytosis; neurotransmitter release; development; synaptogenesis; in situ hybridization

This article has been cited by other articles:

				D. Doischer, J. Aurel Hosp, Y. Yanagawa, K. Obata, P. Jonas, I. Vida, and M. Bartos Postnatal Differentiation of Basket Cells from Slow to Fast Signaling Devices J. Neurosci., November 26, 2008; 28(48): 12956 - 12968. [Abstract] [Full Text] [PDF]

				L. Cnops, T.-T. Hu, K. Burnat, and L. Arckens Influence of Binocular Competition on the Expression Profiles of CRMP2, CRMP4, Dyn I, and Syt I in Developing Cat Visual Cortex Cereb Cortex, May 1, 2008; 18(5): 1221 - 1231. [Abstract] [Full Text] [PDF]

				C. Delaloy, J. Hadchouel, M. Imbert-Teboul, M. Clemessy, A.-M. Houot, and X. Jeunemaitre Cardiovascular Expression of the Mouse WNK1 Gene during Development and Adulthood Revealed by a BAC Reporter Assay Am. J. Pathol., July 1, 2006; 169(1): 105 - 118. [Abstract] [Full Text] [PDF]

				G. J. Murphy, L. L. Glickfeld, Z. Balsen, and J. S. Isaacson Sensory Neuron Signaling to the Brain: Properties of Transmitter Release from Olfactory Nerve Terminals J. Neurosci., March 24, 2004; 24(12): 3023 - 3030. [Abstract] [Full Text] [PDF]

				M. Fukuda, E. Kanno, Y. Ogata, C. Saegusa, T. Kim, Y. P. Loh, and A. Yamamoto Nerve Growth Factor-dependent Sorting of Synaptotagmin IV Protein to Mature Dense-core Vesicles That Undergo Calcium-dependent Exocytosis in PC12 Cells J. Biol. Chem., January 24, 2003; 278(5): 3220 - 3226. [Abstract] [Full Text] [PDF]

				D. Wang and L. Maler Differential roles of Ca2+/calmodulin-dependent kinases in posttetanic potentiation at input selective glutamatergic pathways PNAS, June 9, 1998; 95(12): 7133 - 7138. [Abstract] [Full Text] [PDF]

				K. Palm, N. Belluardo, M. Metsis, and T o. Timmusk Neuronal Expression of Zinc Finger Transcription Factor REST/NRSF/XBR Gene J. Neurosci., February 15, 1998; 18(4): 1280 - 1296. [Abstract] [Full Text] [PDF]

				G. D. Ferguson, X.-N. Chen, J. R. Korenberg, and H. R. Herschman The Human Synaptotagmin IV Gene Defines an Evolutionary Break Point between Syntenic Mouse and Human Chromosome Regions but Retains Ligand Inducibility and Tissue Specificity J. Biol. Chem., November 17, 2000; 275(47): 36920 - 36926. [Abstract] [Full Text] [PDF]

				G. D. Ferguson, S. G. Anagnostaras, A. J. Silva, and H. R. Herschman Deficits in memory and motor performance in synaptotagmin IV mutant mice PNAS, May 9, 2000; 97(10): 5598 - 5603. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help