WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Discover www.zeiss.de/functionality
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (112)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jordán, J.
Right arrow Articles by Miller, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jordán, J.
Right arrow Articles by Miller, R. J.

 Previous Article  |  Next Article 

Volume 17, Number 4, Issue of February 15, 1997 pp. 1397-1405
Copyright ©1997 Society for Neuroscience

p53 Expression Induces Apoptosis in Hippocampal Pyramidal Neuron Cultures

Received Oct. 1, 1996; revised Nov. 27, 1996; accepted Dec. 4, 1996.

Joaquín Jordán1, María F. Galindo1, Jochen H. M. Prehn1, Ralph R. Weichselbaum2, Michael Beckett2, Ghanashyam D. Ghadge3, Raymond P. Roos3, Jeffrey M. Leiden4, and Richard J. Miller1

Departments of 1 Pharmacological and Physiological Sciences, 2 Radiation and Cell Oncology, 3 Neurology, and 4 Medicine, The University of Chicago, Chicago, Illinois 60637

The tumor suppressor gene p53 has been implicated in the induction of apoptosis in dividing cells. We now show that overexpression of p53 using an adenoviral vector in cultured rat hippocampal pyramidal neurons causes widespread neuronal death with features typical of apoptosis. p53 overexpression did not induce p21, bax, or mdm2 in neurons. X-irradiation of hippocampal neurons induced p53 immunoreactivity and cell death associated with features typical of apoptosis. Overexpression of a constitutively active nonphosphorylatable form of the retinoblastoma gene product blocked x-irradiation-induced neuronal death. However, overexpression of the cyclin-dependent kinase inhibitor p21 did not. Treatment of neurons with transforming growth factor-beta 1 protected them from x-irradiation. These results are consistent with a role for p53 in nerve cell death that is distinct from its actions relating to cell cycle arrest.

Key words: adenovirus; irradiation; retinoblastoma; tumor suppressor genes; overexpression; p21; transforming growth factor-beta 1




This article has been cited by other articles:


Home page
 J. Neurosci.Home page
T. Uo, Y. Kinoshita, and R. S. Morrison
Apoptotic Actions of p53 Require Transcriptional Activation of PUMA and Do Not Involve a Direct Mitochondrial/Cytoplasmic Site of Action in Postnatal Cortical Neurons
J. Neurosci., November 7, 2007; 27(45): 12198 - 12210.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
A. J. Chucair, N. P. Rotstein, J. P. SanGiovanni, A. During, E. Y. Chew, and L. E. Politi
Lutein and Zeaxanthin Protect Photoreceptors from Apoptosis Induced by Oxidative Stress: Relation with Docosahexaenoic Acid
Invest. Ophthalmol. Vis. Sci., November 1, 2007; 48(11): 5168 - 5177.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Endo, H. Kamada, C. Nito, T. Nishi, and P. H. Chan
Mitochondrial translocation of p53 mediates release of cytochrome c and hippocampal CA1 neuronal death after transient global cerebral ischemia in rats.
J. Neurosci., July 26, 2006; 26(30): 7974 - 7983.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. D. Pozniak, F. Barnabe-Heider, V. V. Rymar, A. F. Lee, A. F. Sadikot, and F. D. Miller
p73 Is Required for Survival and Maintenance of CNS Neurons
J. Neurosci., November 15, 2002; 22(22): 9800 - 9809.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
C. CROCHEMORE, T. M. MICHAELIDIS, D. FISCHER, J.-P. LOEFFLER, and O. F. X. ALMEIDA
Enhancement of p53 activity and inhibition of neural cell proliferation by glucocorticoid receptor activation
FASEB J, June 1, 2002; 16(8): 761 - 770.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
A. G. ESTEVEZ and J. JORDAN
Nitric Oxide and Superoxide, a Deadly Cocktail
Ann. N.Y. Acad. Sci., May 1, 2002; 962(1): 207 - 211.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Yamaguchi, M. Taniguchi, O. Hori, S. Ogawa, N. Tojo, N. Matsuoka, S.-i. Miyake, K. Kasai, H. Sugimoto, M. Tamatani, et al.
Peg3/Pw1 Is Involved in p53-mediated Cell Death Pathway in Brain Ischemia/Hypoxia
J. Biol. Chem., January 4, 2002; 277(1): 623 - 629.
[Abstract] [Full Text]

Home page
 IOVSHome page
L. E. Politi, N. P. Rotstein, and N. G. Carri
Effect of GDNF on Neuroblast Proliferation and Photoreceptor Survival: Additive Protection with Docosahexaenoic Acid
Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 3008 - 3015.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Shinoura, Y. Muramatsu, M. Nishimura, Y. Yoshida, A. Saito, T. Yokoyama, T. Furukawa, A. Horii, M. Hashimoto, A. Asai, et al.
Adenovirus-mediated Transfer of p33ING1 with p53 Drastically Augments Apoptosis in Gliomas
Cancer Res., November 1, 1999; 59(21): 5521 - 5528.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
R.-W. Chen, P. A. Saunders, H. Wei, Z. Li, P. Seth, and D.-M. Chuang
Involvement of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and p53 in Neuronal Apoptosis: Evidence That GAPDH Is Upregulated by p53
J. Neurosci., November 1, 1999; 19(21): 9654 - 9662.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
S. P. Cregan, J. G. MacLaurin, C. G. Craig, G. S. Robertson, D. W. Nicholson, D. S. Park, and R. S. Slack
Bax-Dependent Caspase-3 Activation Is a Key Determinant in p53-Induced Apoptosis in Neurons
J. Neurosci., September 15, 1999; 19(18): 7860 - 7869.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Shinoura, Y. Yoshida, R. Tsunoda, M. Ohashi, W. Zhang, A. Asai, T. Kirino, and H. Hamada
Highly Augmented Cytopathic Effect of a Fiber-mutant E1B-defective Adenovirus for Gene Therapy of Gliomas
Cancer Res., July 1, 1999; 59(14): 3411 - 3416.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
X. Xu, D. Yang, T. Wyss-Coray, J. Yan, L. Gan, Y. Sun, and L. Mucke
Wild-type but not Alzheimer-mutant amyloid precursor protein confers resistance against p53-mediated apoptosis
PNAS, June 22, 1999; 96(13): 7547 - 7552.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Taniura, K. Matsumoto, and K. Yoshikawa
Physical and Functional Interactions of Neuronal Growth Suppressor Necdin with p53
J. Biol. Chem., June 4, 1999; 274(23): 16242 - 16248.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
Z.-H. Qin, R.-W. Chen, Y. Wang, M. Nakai, D.-M. Chuang, and T. N. Chase
Nuclear Factor kappa B Nuclear Translocation Upregulates c-Myc and p53 Expression during NMDA Receptor-Mediated Apoptosis in Rat Striatum
J. Neurosci., May 15, 1999; 19(10): 4023 - 4033.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
M. D. Johnson, Y. Kinoshita, H. Xiang, S. Ghatan, and R. S. Morrison
Contribution of p53-Dependent Caspase Activation to Neuronal Cell Death Declines with Neuronal Maturation
J. Neurosci., April 15, 1999; 19(8): 2996 - 3006.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
C. N. G. Anderson and A. M. Tolkovsky
A Role for MAPK/ERK in Sympathetic Neuron Survival: Protection against a p53-Dependent, JNK-Independent Induction of Apoptosis by Cytosine Arabinoside
J. Neurosci., January 15, 1999; 19(2): 664 - 673.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
C. Ghiani, A. Eisen, X Yuan, R. DePinho, C. McBain, and V Gallo
Neurotransmitter receptor activation triggers p27(Kip1 )and p21(CIP1) accumulation and G1 cell cycle arrest in oligodendrocyte progenitors
Development, January 2, 1999; 126(5): 1077 - 1090.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
U. V. Shenoy, E. M. Richards, X.-C. Huang, and C. Sumners
Angiotensin II Type 2 Receptor-Mediated Apoptosis of Cultured Neurons from Newborn Rat Brain
Endocrinology, January 1, 1999; 140(1): 500 - 509.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
G. D. Ghadge, L. Ma, S. Sato, J. Kim, and R. P. Roos
A Protein Critical for a Theiler's Virus-Induced Immune System-Mediated Demyelinating Disease Has a Cell Type-Specific Antiapoptotic Effect and a Key Role in Virus Persistence
J. Virol., November 1, 1998; 72(11): 8605 - 8612.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
F. Berger, F. H. Gage, and S. Vijayaraghavan
Nicotinic Receptor-Induced Apoptotic Cell Death of Hippocampal Progenitor Cells
J. Neurosci., September 1, 1998; 18(17): 6871 - 6881.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurosci.Home page
H. Xiang, Y. Kinoshita, C. M. Knudson, S. J. Korsmeyer, P. A. Schwartzkroin, and R. S. Morrison
Bax Involvement in p53-Mediated Neuronal Cell Death
J. Neurosci., February 15, 1998; 18(4): 1363 - 1373.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Lakkaraju, J. M. Dubinsky, W. C. Low, and Y.-E. Rahman
Neurons Are Protected from Excitotoxic Death by p53 Antisense Oligonucleotides Delivered in Anionic Liposomes
J. Biol. Chem., August 17, 2001; 276(34): 32000 - 32007.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-