QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (19) Citing Articles via Google Scholar Google Scholar Articles by Kind, P. C. Articles by Hockfield, S. Search for Related Content PubMed PubMed Citation Articles by Kind, P. C. Articles by Hockfield, S.

 Previous Article  |  Next Article 

Volume 17, Number 4, Issue of February 15, 1997 pp. 1471-1480
Copyright ©1997 Society for Neuroscience

Phospholipase C-1 Is Present in the Botrysome, an Intermediate Compartment-Like Organelle, and Is Regulated by Visual Experience in Cat Visual Cortex

Received Sept. 18, 1996; revised Nov. 18, 1996; accepted Dec. 2, 1996.

Peter C. Kind¹, Gail M. Kelly¹, Hugh J. L. Fryer¹, Colin Blakemore², and Susan Hockfield¹

¹ Section of Neurobiology, Yale University School of Medicine, New Haven, Connecticut 06511, and ² University Laboratory of Physiology, Oxford, OX1 3PT, United Kingdom OX1 3PT

Monoclonal antibody Cat-307 identifies a 165 kDa neuronal protein expressed in the cat visual cortex during the period of sensitivity to alterations in visual experience (). Dark-rearing, which prolongs the sensitive period, also prolongs the expression of the Cat-307 protein. The Cat-307 protein localizes to an organelle, here called the botrysome (from the Greek botrys, cluster of grapes), that is located between the endoplasmic reticulum (ER) and Golgi apparatus. The botrysome is composed of small ring-shaped profiles with electron-dense coats. The size and morphology of the rings and their coats are similar to those described for ER to Golgi transport vesicles. Biochemically, the Cat-307 protein cofractionates with microsomes and partitions with subunits of the coatomer proteins that coat ER-to-Golgi transport vesicles. Partial amino acid sequencing reveals that the Cat-307 protein is phospholipase C-1, the G-protein-dependent phosphodiesterase that hydrolyses phosphatidylinositol 4,5 biphosphate into inositol 1,4,5 triphosphate and diacylglycerol after the stimulation of a variety of neurotransmitter receptors at the cell surface. These results suggest a role for phospholipase C-1 and the botrysome in developmental plasticity and provide a possible link between receptor activation at the cell surface and protein transport during neuronal development.

Key words: Cat-307, phospholipase C, Golgi apparatus, endoplasmic reticulum, dark-rearing, protein transport, transport vesicles, critical period

This article has been cited by other articles:

				M. Liao, J. Shen, Y. Zhang, S.-H. Li, X.-J. Li, and H. Li Immunohistochemical Localization of Huntingtin-associated Protein 1 in Endocrine System of the Rat J. Histochem. Cytochem., December 1, 2005; 53(12): 1517 - 1524. [Abstract] [Full Text] [PDF]

				T. L. Spires, Z. Molnar, P. C. Kind, P. M. Cordery, A. L. Upton, C. Blakemore, and A. J. Hannan Activity-dependent Regulation of Synapse and Dendritic Spine Morphology in Developing Barrel Cortex Requires Phospholipase C-{beta}1 Signalling Cereb Cortex, April 1, 2005; 15(4): 385 - 393. [Abstract] [Full Text] [PDF]

				C.-A. Gutekunst, S.-H. Li, H. Yi, R. J. Ferrante, X.-J. Li, and S. M. Hersch The Cellular and Subcellular Localization of Huntingtin-Associated Protein 1 (HAP1): Comparison with Huntingtin in Rat and Human J. Neurosci., October 1, 1998; 18(19): 7674 - 7686. [Abstract] [Full Text] [PDF]

				C. Smyth, G. Logan, R. P. Weinberger, P. B. Rowe, I. E. Alexander, and J. A. Smythe Identification of a Dynamic Intracellular Reservoir of CD86 Protein in Peripheral Blood Monocytes That Is Not Associated with the Golgi Complex J. Immunol., June 1, 1998; 160(11): 5390 - 5396. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help