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Volume 17, Number 6, Issue of March 15, 1997 pp. 2079-2087
Copyright ©1997 Society for Neuroscience

Local Homogeneity of Cell Cycle Length in Developing Mouse Cortex

Received Aug. 5, 1996; revised Dec. 17, 1996; accepted Dec. 20, 1996.

Li Cai^{1, 2}, Nancy L. Hayes¹, and Richard S. Nowakowski^{1, 2}

¹ Department of Neuroscience and Cell Biology and ² Physiology and Neurobiology Graduate Program, Rutgers University and University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854

We have measured the amount of variation in the length of the cell cycle for cells in the pseudostratified ventricular epithelium (PVE) of the developing cortex of mice on embryonic day 14. Our measurements were made in three cortical regions (i.e., the neocortex, archicortex, and periarchicortex) using three different methods: the cumulative labeling method (CLM), the percent labeled mitoses (PLM) method, and a comparison of the time needed for the PLM to ascend from 0 to 100% with the time needed for the PLM to descend from 100 to 0%. These 3 different techniques provide different perspectives on the cytokinetic parameters. Theoretically, CLM gives an estimate for a maximum value of the total length of the cell cycle (T_C), whereas PLM gives an estimate of a minimum value of T_C. The difference between these two estimates indicates that the range for T_C is ±1% of the mean T_C for periarchicortex, ±7% for neocortex, and ±8% for archicortex. This was confirmed by a lengthening of the PLM descent time in comparison with its ascent time. The sharpness of the transitions and the flatness of the plateau of the PLM curves indicate that 99% of the proliferating cells are within this narrow estimated range for T_C; hence, only ~1% deviate outside of a relatively restricted range from the average T_C of the population. In the context of the possible existence within the cortical PVE of two populations with markedly dissimilar cell cycle kinetics from the mean, one such population must comprise ~99% of the total population, and the other, if it exists, is only ~1% of the total. This seems to be true for all three cortical regions. The narrow range of T_C indicates a homogeneity in the cell cycle length for proliferating cells in three different cortical regions, despite the fact that progenitor cells of different lineages may be present. It further predicts the existence of almost synchronous interkinetic nuclear movements of the proliferating cells in the ventricular zone during early development of the cerebral cortex.

Key words: neuronogenesis; mouse; cell proliferation; ventricular zone; S phase labeling; bromodeoxyuridine

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