 |
|||||
|
|||||
|
|||||
|
|||||
|
|||||
Previous Article | Next Article
Volume 17, Number 7, Issue of April 1, 1997 pp. 2314-2323
Copyright ©1997 Society for NeuroscienceRapid Exocytosis in Single Chromaffin Cells Recorded from Mouse Adrenal Slices
Received Dec. 3, 1996; accepted Jan. 21, 1997.
Tobias Moser and Erwin Neher Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Faßberg, D-37077, Göttingen, Germany
We report here that brief depolarizations such as action potentials trigger exocytosis in thin mouse adrenal slices. The secretory rates obtained in membrane capacitance recordings from chromaffin cells in slices are faster than those observed in isolated cells. Fast exocytosis in slices is attributable to the rapid release of a small pool of vesicles. The pool recovers from depletion with a time constant of 10 sec. Recruitment of the rapidly released vesicles is strongly hindered by the fast Ca2+ chelator BAPTA and much less by the slower chelator EGTA. We suggest that these vesicles are located in close proximity to Ca2+ channels. Spatial coupling of Ca2+ entry and exocytosis may be sensitive to cell isolation and culture.
Key words: exocytosis; membrane capacitance measurement; chromaffin; adrenal slice; calcium chelators; calcium-secretion coupling; neuroendocrine; calcium current; secretory depression
This article has been cited by other articles:
M. M. Wu, A. Llobet, and L. Lagnado
Loose coupling between calcium channels and sites of exocytosis in chromaffin cells
J. Physiol., November 15, 2009; 587(22): 5377 - 5391.
[Abstract] [Full Text] [PDF]
S. Misler
Unifying concepts in stimulus-secretion coupling in endocrine cells and some implications for therapeutics
Advan Physiol Educ, September 1, 2009; 33(3): 175 - 186.
[Abstract] [Full Text] [PDF]
I. Iankova, C. Chavey, C. Clape, C. Colomer, N. C. Guerineau, N. Grillet, J.-F. Brunet, J.-S. Annicotte, and L. Fajas
Regulator of G Protein Signaling-4 Controls Fatty Acid and Glucose Homeostasis
Endocrinology, November 1, 2008; 149(11): 5706 - 5712.
[Abstract] [Full Text] [PDF]
X.-W. Chen, Y.-Q. Feng, C.-J. Hao, X.-L. Guo, X. He, Z.-Y. Zhou, N. Guo, H.-P. Huang, W. Xiong, H. Zheng, et al.
DTNBP1, a schizophrenia susceptibility gene, affects kinetics of transmitter release
J. Cell Biol., October 20, 2008; 181(5): 791 - 801.
[Abstract] [Full Text] [PDF]
Y. Liu, C. Schirra, D. R. Stevens, U. Matti, D. Speidel, D. Hof, D. Bruns, N. Brose, and J. Rettig
CAPS Facilitates Filling of the Rapidly Releasable Pool of Large Dense-Core Vesicles
J. Neurosci., May 21, 2008; 28(21): 5594 - 5601.
[Abstract] [Full Text] [PDF]
A. Neef, D. Khimich, P. Pirih, D. Riedel, F. Wolf, and T. Moser
Probing the Mechanism of Exocytosis at the Hair Cell Ribbon Synapse
J. Neurosci., November 21, 2007; 27(47): 12933 - 12944.
[Abstract] [Full Text] [PDF]
T. Rose, S. Efendic, and M. Rupnik
Ca2+-Secretion Coupling Is Impaired in Diabetic Goto Kakizaki rats
J. Gen. Physiol., June 1, 2007; 129(6): 493 - 508.
[Abstract] [Full Text] [PDF]
H. Wang, S.-a. Chan, M. Ogier, D. Hellard, Q. Wang, C. Smith, and D. M. Katz
Dysregulation of brain-derived neurotrophic factor expression and neurosecretory function in mecp2 null mice.
J. Neurosci., October 18, 2006; 26(42): 10911 - 10915.
[Abstract] [Full Text] [PDF]
I. Dudanova, S. Sedej, M. Ahmad, H. Masius, V. Sargsyan, W. Zhang, D. Riedel, F. Angenstein, D. Schild, M. Rupnik, et al.
Important Contribution of {alpha}-Neurexins to Ca2+-Triggered Exocytosis of Secretory Granules
J. Neurosci., October 11, 2006; 26(41): 10599 - 10613.
[Abstract] [Full Text] [PDF]
A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho
Calcium signaling and exocytosis in adrenal chromaffin cells.
Physiol Rev, October 1, 2006; 86(4): 1093 - 1131.
[Abstract] [Full Text] [PDF]
G. Arroyo, J. Fuentealba, N. Sevane-Fernandez, M. Aldea, A. G. Garcia, and A. Albillos
Amperometric Study of the Kinetics of Exocytosis in Mouse Adrenal Slice Chromaffin Cells: Physiological and Methodological Insights
J Neurophysiol, September 1, 2006; 96(3): 1196 - 1202.
[Abstract] [Full Text] [PDF]
J. Xu, K. S. Tang, V. B. Lu, C. P. Weerasinghe, A. Tse, and F. W. Tse
Maintenance of quantal size and immediately releasable granules in rat chromaffin cells by glucocorticoid
Am J Physiol Cell Physiol, November 1, 2005; 289(5): C1122 - C1133.
[Abstract] [Full Text] [PDF]
S. Sedej, T. Rose, and M. Rupnik
cAMP increases Ca2+-dependent exocytosis through both PKA and Epac2 in mouse melanotrophs from pituitary tissue slices
J. Physiol., September 15, 2005; 567(3): 799 - 813.
[Abstract] [Full Text] [PDF]
S.-A. Chan, L. Polo-Parada, L. T. Landmesser, and C. Smith
Adrenal Chromaffin Cells Exhibit Impaired Granule Trafficking in NCAM Knockout Mice
J Neurophysiol, August 1, 2005; 94(2): 1037 - 1047.
[Abstract] [Full Text] [PDF]
H. Yang, H. Liu, Z. Hu, H. Zhu, and T. Xu
PKC-induced Sensitization of Ca2+-dependent Exocytosis Is Mediated by Reducing the Ca2+ Cooperativity in Pituitary Gonadotropes
J. Gen. Physiol., February 28, 2005; 125(3): 327 - 334.
[Abstract] [Full Text] [PDF]
S. Gopel, Q. Zhang, L. Eliasson, X.-S. Ma, J. Galvanovskis, T. Kanno, A. Salehi, and P. Rorsman
Capacitance measurements of exocytosis in mouse pancreatic {alpha}-, {beta}- and {delta}-cells within intact islets of Langerhans
J. Physiol., May 1, 2004; 556(3): 711 - 726.
[Abstract] [Full Text] [PDF]
S.-A. Chan and C. Smith
Low frequency stimulation of mouse adrenal slices reveals a clathrin-independent, protein kinase C-mediated endocytic mechanism
J. Physiol., December 15, 2003; 553(3): 707 - 717.
[Abstract] [Full Text] [PDF]
Y. Yang, S. Udayasankar, J. Dunning, P. Chen, and K. D. Gillis
A highly Ca2+-sensitive pool of vesicles is regulated by protein kinase C in adrenal chromaffin cells
PNAS, December 24, 2002; 99(26): 17060 - 17065.
[Abstract] [Full Text] [PDF]
A. O. Martin, M.-N. Mathieu, C. Chevillard, and N. C. Guerineau
Gap Junctions Mediate Electrical Signaling and Ensuing Cytosolic Ca2+ Increases between Chromaffin Cells in Adrenal Slices: A Role in Catecholamine Release
J. Neurosci., August 1, 2001; 21(15): 5397 - 5405.
[Abstract] [Full Text] [PDF]
J. K. Angleson and W. J. Betz
Intraterminal Ca2+ and Spontaneous Transmitter Release at the Frog Neuromuscular Junction
J Neurophysiol, January 1, 2001; 85(1): 287 - 294.
[Abstract] [Full Text] [PDF]
A. Albillos, E. Neher, and T. Moser
R-Type Ca2+ Channels Are Coupled to the Rapid Component of Secretion in Mouse Adrenal Slice Chromaffin Cells
J. Neurosci., November 15, 2000; 20(22): 8323 - 8330.
[Abstract] [Full Text] [PDF]
V. Dinkelacker, T. Voets, E. Neher, and T. Moser
The Readily Releasable Pool of Vesicles in Chromaffin Cells Is Replenished in a Temperature-Dependent Manner and Transiently Overfills at 37{degrees}C
J. Neurosci., November 15, 2000; 20(22): 8377 - 8383.
[Abstract] [Full Text] [PDF]
A. Tse and A. K Lee
Voltage-gated Ca2+ channels and intracellular Ca2+ release regulate exocytosis in identified rat corticotrophs
J. Physiol., October 1, 2000; 528(1): 79 - 90.
[Abstract] [Full Text] [PDF]
T. Moser and D. Beutner
Kinetics of exocytosis and endocytosis at the cochlear inner hair cell afferent synapse of the mouse
PNAS, January 18, 2000; 97(2): 883 - 888.
[Abstract] [Full Text] [PDF]
S Gopel, T Kanno, S Barg, J Galvanovskis, and P Rorsman
Voltage-gated and resting membrane currents recorded from B-cells in intact mouse pancreatic islets
J. Physiol., December 15, 1999; 521(3): 717 - 728.
[Abstract] [Full Text] [PDF]
F. Amzica and D. Neckelmann
Membrane Capacitance of Cortical Neurons and Glia During Sleep Oscillations and Spike-Wave Seizures
J Neurophysiol, November 1, 1999; 82(5): 2731 - 2746.
[Abstract] [Full Text] [PDF]
M. Villarroya, R. Olivares, A. Ruiz, M. F Cano-Abad, R. de Pascual, R. B Lomax, M. G Lopez, I. Mayorgas, L. Gandia, and A. G Garcia
Voltage inactivation of Ca2+ entry and secretion associated with N- and P/Q-type but not L-type Ca2+ channels of bovine chromaffin cells
J. Physiol., April 15, 1999; 516(2): 421 - 432.
[Abstract] [Full Text] [PDF]
C. Smith
A Persistent Activity-Dependent Facilitation in Chromaffin Cells Is Caused by Ca2+ Activation of Protein Kinase C
J. Neurosci., January 15, 1999; 19(2): 589 - 598.
[Abstract] [Full Text] [PDF]
A. Elhamdani, Z. Zhou, and C. R. Artalejo
Timing of Dense-Core Vesicle Exocytosis Depends on the Facilitation L-Type Ca Channel in Adrenal Chromaffin Cells
J. Neurosci., August 15, 1998; 18(16): 6230 - 6240.
[Abstract] [Full Text] [PDF]
N. C. Guerineau, X. Bonnefont, L. Stoeckel, and P. Mollard
Synchronized Spontaneous Ca2+ Transients in Acute Anterior Pituitary Slices
J. Biol. Chem., April 24, 1998; 273(17): 10389 - 10395.
[Abstract] [Full Text] [PDF]
T. Moser
Low-conductance intercellular coupling between mouse chromaffin cells in situ
J. Physiol., January 1, 1998; 506(1): 195 - 205.
[Abstract] [Full Text] [PDF]
S. Mennerick, D. Zenisek, and G. Matthews
Static and Dynamic Membrane Properties of Large-Terminal Bipolar Cells From Goldfish Retina: Experimental Test of a Compartment Model
J Neurophysiol, July 1, 1997; 78(1): 51 - 62.
[Abstract] [Full Text] [PDF]
T. Moser and E. Neher
Estimation of mean exocytic vesicle capacitance in mouse adrenal chromaffin cells
PNAS, June 24, 1997; 94(13): 6735 - 6740.
[Abstract] [Full Text] [PDF]
E. Ales, N. H. Gabilan, M. F. Cano-Abad, A. G. Garcia, and M. G. Lopez
The Sea Anemone Toxin Bc2 Induces Continuous or Transient Exocytosis, in the Presence of Sustained Levels of High Cytosolic Ca2+ in Chromaffin Cells
J. Biol. Chem., November 22, 2000; 275(48): 37488 - 37495.
[Abstract] [Full Text] [PDF]
J. E. Keller and E. A. Neale
The Role of the Synaptic Protein SNAP-25 in the Potency of Botulinum Neurotoxin Type A
J. Biol. Chem., April 13, 2001; 276(16): 13476 - 13482.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|