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Volume 17, Number 7, Issue of April 1, 1997 pp. 2314-2323
Copyright ©1997 Society for Neuroscience

Rapid Exocytosis in Single Chromaffin Cells Recorded from Mouse Adrenal Slices

Received Dec. 3, 1996; accepted Jan. 21, 1997.

Tobias Moser and Erwin Neher

Department of Membrane Biophysics, Max-Planck-Institute for Biophysical Chemistry, Am Faßberg, D-37077, Göttingen, Germany

We report here that brief depolarizations such as action potentials trigger exocytosis in thin mouse adrenal slices. The secretory rates obtained in membrane capacitance recordings from chromaffin cells in slices are faster than those observed in isolated cells. Fast exocytosis in slices is attributable to the rapid release of a small pool of vesicles. The pool recovers from depletion with a time constant of 10 sec. Recruitment of the rapidly released vesicles is strongly hindered by the fast Ca²⁺ chelator BAPTA and much less by the slower chelator EGTA. We suggest that these vesicles are located in close proximity to Ca²⁺ channels. Spatial coupling of Ca²⁺ entry and exocytosis may be sensitive to cell isolation and culture.

Key words: exocytosis; membrane capacitance measurement; chromaffin; adrenal slice; calcium chelators; calcium-secretion coupling; neuroendocrine; calcium current; secretory depression

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