 |
 Previous Article | Next Article 
Volume 17, Number 7,
Issue of April 1, 1997
pp. 2348-2354
Copyright ©1997 Society for Neuroscience
Nucleus-Specific Chloride Homeostasis in Rat Thalamus
Received Dec. 17, 1996; revised Jan. 17, 1997; accepted Jan. 22, 1997.
Daniel Ulrich and
John R. Huguenard
Department of Neurology and Neurological Sciences, Stanford
University School of Medicine, Stanford, California 94305
Synchronous thalamic network activity occurring during slow wave
sleep and paroxysmal discharges critically depends on the ability of
thalamocortical relay cells and inhibitory neurons of the nucleus
reticularis thalami (nRt) to fire bursts of action potentials.
Inhibitory synaptic potentials (IPSPs) originating from nRt cells are
crucial in deinactivating T-channels and thus promoting burst firing in
relay cells, but the functional role of intra-nRt IPSPs is less well
understood. A major factor that regulates the net effects of IPSP
generation is the chloride equilibrium potential
(ECl). Here we applied the perforated
patch-clamp technique, using the cation-selective ionophore gramicidin
to assess the reversal potential of chloride in nRt and relay cells in
brain slices. We found that the reversal potential of GABA-induced
membrane currents (EGABA) was
significantly more hyperpolarized in relay ( 81 ± 2.6 mV), as compared with nRt cells (71 ± 2.5 mV).
EGABA was not significantly different from
the reversal potential of evoked IPSCs
(EIPSC; 82 ± 4.4 mV) in relay cells.
In both relay and reticular neurons the chloride gradient was collapsed
partially by the chloride cation cotransport blocker furosemide,
suggesting an active chloride extrusion mechanism in thalamic neurons.
Given the relatively hyperpolarized resting potentials (approximately 70 mV) reported for nRt and relay cells during in
vitro thalamic oscillations, we conclude that under these
conditions GABAA IPSPs lead to significant
hyperpolarization in relay cells. By contrast, intra-nRt inhibition
essentially would be shunting, i.e., would produce minimal membrane
polarization but still could reduce the amplitude of excitatory
events.
Key words:
GABA;
IPSP;
somatosensory;
nRt;
perforated patch;
inhibition;
chloride transport
This article has been cited by other articles:
|
|
|
|
 
V. S. Sohal, S. Pangratz-Fuehrer, U. Rudolph, and J. R. Huguenard
Intrinsic and synaptic dynamics interact to generate emergent patterns of rhythmic bursting in thalamocortical neurons.
J. Neurosci.,
April 19, 2006;
26(16):
4247 - 4255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Jia, L. Pignataro, C. M. Schofield, M. Yue, N. L. Harrison, and P. A. Goldstein
An Extrasynaptic GABAA Receptor Mediates Tonic Inhibition in Thalamic VB Neurons
J Neurophysiol,
December 1, 2005;
94(6):
4491 - 4501.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Hill and G. Tononi
Modeling Sleep and Wakefulness in the Thalamocortical System
J Neurophysiol,
March 1, 2005;
93(3):
1671 - 1698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. S. Sohal and J. R. Huguenard
Inhibitory Interconnections Control Burst Pattern and Emergent Network Synchrony in Reticular Thalamus
J. Neurosci.,
October 1, 2003;
23(26):
8978 - 8988.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. DESTEXHE and T. J. SEJNOWSKI
Interactions Between Membrane Conductances Underlying Thalamocortical Slow-Wave Oscillations
Physiol Rev,
October 1, 2003;
83(4):
1401 - 1453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Bacci, J. R. Huguenard, and D. A. Prince
Functional Autaptic Neurotransmission in Fast-Spiking Interneurons: A Novel Form of Feedback Inhibition in the Neocortex
J. Neurosci.,
February 1, 2003;
23(3):
859 - 866.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. J Gentet and D. Ulrich
Strong, reliable and precise synaptic connections between thalamic relay cells and neurones of the nucleus reticularis in juvenile rats
J. Physiol.,
February 1, 2003;
546(3):
801 - 811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. DeFazio, S. Heger, S. R. Ojeda, and S. M. Moenter
Activation of A-Type {gamma}-Aminobutyric Acid Receptors Excites Gonadotropin-Releasing Hormone Neurons
Mol. Endocrinol.,
December 1, 2002;
16(12):
2872 - 2891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bazhenov, I. Timofeev, M. Steriade, and T. J. Sejnowski
Model of Thalamocortical Slow-Wave Sleep Oscillations and Transitions to Activated States
J. Neurosci.,
October 1, 2002;
22(19):
8691 - 8704.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. J. Slaght, N. Leresche, J.-M. Deniau, V. Crunelli, and S. Charpier
Activity of Thalamic Reticular Neurons during Spontaneous Genetically Determined Spike and Wave Discharges
J. Neurosci.,
March 15, 2002;
22(6):
2323 - 2334.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Bevan, P. J. Magill, N. E. Hallworth, J. P. Bolam, and C. J. Wilson
Regulation of the Timing and Pattern of Action Potential Generation in Rat Subthalamic Neurons In Vitro by GABA-A IPSPs
J Neurophysiol,
March 1, 2002;
87(3):
1348 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. De Jeu and C. Pennartz
Circadian Modulation of GABA Function in the Rat Suprachiasmatic Nucleus: Excitatory Effects During the Night Phase
J Neurophysiol,
February 1, 2002;
87(2):
834 - 844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Martina, S. Royer, and D. Pare
Cell-Type-Specific GABA Responses and Chloride Homeostasis in the Cortex and Amygdala
J Neurophysiol,
December 1, 2001;
86(6):
2887 - 2895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Adam, P. G. Finlayson, and D.W.F. Schwarz
Membrane Properties of Principal Neurons of the Lateral Superior Olive
J Neurophysiol,
August 1, 2001;
86(2):
922 - 934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. DeFazio, S. Keros, M. W. Quick, and J. J. Hablitz
Potassium-Coupled Chloride Cotransport Controls Intracellular Chloride in Rat Neocortical Pyramidal Neurons
J. Neurosci.,
November 1, 2000;
20(21):
8069 - 8076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bazhenov, I. Timofeev, M. Steriade, and T. Sejnowski
Spiking-Bursting Activity in the Thalamic Reticular Nucleus Initiates Sequences of Spindle Oscillations in Thalamic Networks
J Neurophysiol,
August 1, 2000;
84(2):
1076 - 1087.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Bevan, C. J. Wilson, J. P. Bolam, and P. J. Magill
Equilibrium Potential of GABAA Current and Implications for Rebound Burst Firing in Rat Subthalamic Neurons In Vitro
J Neurophysiol,
May 1, 2000;
83(5):
3169 - 3172.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. S. Sohal, M. M. Huntsman, and J. R. Huguenard
Reciprocal Inhibitory Connections Regulate the Spatiotemporal Properties of Intrathalamic Oscillations
J. Neurosci.,
March 1, 2000;
20(5):
1735 - 1745.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Zhu and F.-S. Lo
Three GABA Receptor-Mediated Postsynaptic Potentials in Interneurons in the Rat Lateral Geniculate Nucleus
J. Neurosci.,
July 15, 1999;
19(14):
5721 - 5730.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Bevan, P. A. C. Booth, S. A. Eaton, and J. P. Bolam
Selective Innervation of Neostriatal Interneurons by a Subclass of Neuron in the Globus Pallidus of the Rat
J. Neurosci.,
November 15, 1998;
18(22):
9438 - 9452.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. S. Hollrigel, S. T. Ross, and I. Soltesz
Temporal Patterns and Depolarizing Actions of Spontaneous GABAA Receptor Activation in Granule Cells of the Early Postnatal Dentate Gyrus
J Neurophysiol,
November 1, 1998;
80(5):
2340 - 2351.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bazhenov, I. Timofeev, M. Steriade, and T. J. Sejnowski
Computational Models of Thalamocortical Augmenting Responses
J. Neurosci.,
August 15, 1998;
18(16):
6444 - 6465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Debarbieux, J. Brunton, and S. Charpak
Effect of Bicuculline on Thalamic Activity: A Direct Blockade of IAHP in Reticularis Neurons
J Neurophysiol,
June 1, 1998;
79(6):
2911 - 2918.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Bazhenov, I. Timofeev, M. Steriade, and T. J. Sejnowski
Cellular and Network Models for Intrathalamic Augmenting Responses During 10-Hz Stimulation
J Neurophysiol,
May 1, 1998;
79(5):
2730 - 2748.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. V. Sanchez-Vives, T. Bal, and D. A. McCormick
Inhibitory Interactions between Perigeniculate GABAergic Neurons
J. Neurosci.,
November 15, 1997;
17(22):
8894 - 8908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Ulrich and J. R. Huguenard
GABAA-Receptor-Mediated Rebound Burst Firing and Burst Shunting in Thalamus
J Neurophysiol,
September 1, 1997;
78(3):
1748 - 1751.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
