QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (99) Citing Articles via Google Scholar Google Scholar Articles by Challacombe, J. F. Articles by Letourneau, P. C. Search for Related Content PubMed PubMed Citation Articles by Challacombe, J. F. Articles by Letourneau, P. C.

 Previous Article  |  Next Article 

Volume 17, Number 9, Issue of May 1, 1997 pp. 3085-3095
Copyright ©1997 Society for Neuroscience

Dynamic Microtubule Ends Are Required for Growth Cone Turning to Avoid an Inhibitory Guidance Cue

Received Nov. 7, 1996; revised Feb. 5, 1997; accepted Feb. 10, 1997.

Jean F. Challacombe, Diane M. Snow, and Paul C. Letourneau

Department of Cell Biology and Neuroanatomy, The University of Minnesota, Minneapolis, Minnesota 55455

Growth cone turning is an important mechanism for changing the direction of neurite elongation during development of the nervous system. Our previous study indicated that actin filament bundles at the leading margin direct the distal microtubular cytoskeleton as growth cones turn to avoid substratum-bound chondroitin sulfate proteoglycan. Here, we investigated the role of microtubule dynamics in growth cone turning by using low doses of vinblastine and taxol, treatments that reduce dynamic growth and shrinkage of microtubule ends. We used time-lapse phase-contrast videomicroscopy to observe embryonic chick dorsal root ganglion neuronal growth cones as they encountered a border between fibronectin and chondroitin sulfate proteoglycan in the presence and absence of 4 nM vinblastine or 7 nM taxol. Growth cones were fixed and immunocytochemically labeled to identify actin filaments and microtubules containing tyrosinated and detyrosinated -tubulin.

Our results show that after contact with substratum-bound chondroitin sulfate proteoglycan, vinblastine- and taxol-treated growth cones did not turn, as did controls; instead, they stopped or sidestepped. Even before drug-treated growth cones contacted a chondroitin sulfate proteoglycan border, they were narrower than controls, and the distal tyrosinated microtubules were less splayed and were closer to the leading edges of the growth cones. We conclude that the splayed dynamic distal ends of microtubules play a key role in the actin filament-mediated steering of growth cone microtubules to produce growth cone turning.

Key words: microtubule; growth cone; turning; actin filament; chondroitin sulfate proteoglycan; dynamic instability

This article has been cited by other articles:

				S. Geraldo and P. R. Gordon-Weeks Cytoskeletal dynamics in growth-cone steering J. Cell Sci., October 15, 2009; 122(20): 3595 - 3604. [Abstract] [Full Text] [PDF]

				T. Shintani, M. Ihara, S. Tani, J. Sakuraba, H. Sakuta, and M. Noda APC2 Plays an Essential Role in Axonal Projections through the Regulation of Microtubule Stability J. Neurosci., September 16, 2009; 29(37): 11628 - 11640. [Abstract] [Full Text] [PDF]

				F. Mimura, S. Yamagishi, N. Arimura, M. Fujitani, T. Kubo, K. Kaibuchi, and T. Yamashita Myelin-associated Glycoprotein Inhibits Microtubule Assembly by a Rho-kinase-dependent Mechanism J. Biol. Chem., June 9, 2006; 281(23): 15970 - 15979. [Abstract] [Full Text] [PDF]

				A. M. Rajnicek, L. E. Foubister, and C. D. McCaig Growth cone steering by a physiological electric field requires dynamic microtubules, microfilaments and Rac-mediated filopodial asymmetry J. Cell Sci., May 1, 2006; 119(9): 1736 - 1745. [Abstract] [Full Text] [PDF]

				M. L. Lemons, S. Barua, M. L. Abanto, W. Halfter, and M. L. Condic Adaptation of Sensory Neurons to Hyalectin and Decorin Proteoglycans J. Neurosci., May 18, 2005; 25(20): 4964 - 4973. [Abstract] [Full Text] [PDF]

				N. Trivedi, P. Marsh, R. G. Goold, A. Wood-Kaczmar, and P. R. Gordon-Weeks Glycogen synthase kinase-3{beta} phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons J. Cell Sci., March 1, 2005; 118(5): 993 - 1005. [Abstract] [Full Text] [PDF]

				C. Bouquet, S. Soares, Y. von Boxberg, M. Ravaille-Veron, F. Propst, and F. Nothias Microtubule-Associated Protein 1B Controls Directionality of Growth Cone Migration and Axonal Branching in Regeneration of Adult Dorsal Root Ganglia Neurons J. Neurosci., August 11, 2004; 24(32): 7204 - 7213. [Abstract] [Full Text] [PDF]

				L. J. Turner, S. Nicholls, and A. Hall The Activity of the Plexin-A1 Receptor Is Regulated by Rac J. Biol. Chem., August 6, 2004; 279(32): 33199 - 33205. [Abstract] [Full Text] [PDF]

				L. H. Suh, S. F. Oster, S. S. Soehrman, G. Grenningloh, and D. W. Sretavan L1/Laminin Modulation of Growth Cone Response to EphB Triggers Growth Pauses and Regulates the Microtubule Destabilizing Protein SCG10 J. Neurosci., February 25, 2004; 24(8): 1976 - 1986. [Abstract] [Full Text] [PDF]

				K. B. Buck and J. Q. Zheng Growth Cone Turning Induced by Direct Local Modification of Microtubule Dynamics J. Neurosci., November 1, 2002; 22(21): 9358 - 9367. [Abstract] [Full Text] [PDF]

				A. W. Schaefer, N. Kabir, and P. Forscher Filopodia and actin arcs guide the assembly and transport of two populations of microtubules with unique dynamic parameters in neuronal growth cones J. Cell Biol., July 8, 2002; 158(1): 139 - 152. [Abstract] [Full Text] [PDF]

				F.-Q. Zhou, C. M. Waterman-Storer, and C. S. Cohan Focal loss of actin bundles causes microtubule redistribution and growth cone turning J. Cell Biol., May 28, 2002; 157(5): 839 - 849. [Abstract] [Full Text] [PDF]

				A. B. Nixon, G. Grenningloh, and P. J. Casey The Interaction of RGSZ1 with SCG10 Attenuates the Ability of SCG10 to Promote Microtubule Disassembly J. Biol. Chem., May 10, 2002; 277(20): 18127 - 18133. [Abstract] [Full Text] [PDF]

				M. D. Kim, P. Kolodziej, and A. Chiba Growth Cone Pathfinding and Filopodial Dynamics Are Mediated Separately by Cdc42 Activation J. Neurosci., March 1, 2002; 22(5): 1794 - 1806. [Abstract] [Full Text] [PDF]

				E. W. Dent and K. Kalil Axon Branching Requires Interactions between Dynamic Microtubules and Actin Filaments J. Neurosci., December 15, 2001; 21(24): 9757 - 9769. [Abstract] [Full Text] [PDF]

				N. Kabir, A. W. Schaefer, A. Nakhost, W. S. Sossin, and P. Forscher Protein Kinase C Activation Promotes Microtubule Advance in Neuronal Growth Cones by Increasing Average Microtubule Growth Lifetimes J. Cell Biol., March 5, 2001; 152(5): 1033 - 1044. [Abstract] [Full Text] [PDF]

				H. Kamiguchi and V. Lemmon Recycling of the Cell Adhesion Molecule L1 in Axonal Growth Cones J. Neurosci., May 15, 2000; 20(10): 3676 - 3686. [Abstract] [Full Text] [PDF]

				M. L. Condic, D. M. Snow, and P. C. Letourneau Embryonic Neurons Adapt to the Inhibitory Proteoglycan Aggrecan by Increasing Integrin Expression J. Neurosci., November 15, 1999; 19(22): 10036 - 10043. [Abstract] [Full Text] [PDF]

				G. Gallo and P. C. Letourneau Different Contributions of Microtubule Dynamics and Transport to the Growth of Axons and Collateral Sprouts J. Neurosci., May 15, 1999; 19(10): 3860 - 3873. [Abstract] [Full Text] [PDF]

				R. Goold, R Owen, and P. Gordon-Weeks Glycogen synthase kinase 3beta phosphorylation of microtubule-associated protein 1B regulates the stability of microtubules in growth cones J. Cell Sci., January 10, 1999; 112(19): 3373 - 3384. [Abstract] [PDF]

				A. Prokop, J. Uhler, J. Roote, and M. Bate The kakapo Mutation Affects Terminal Arborization and Central Dendritic Sprouting of Drosophila Motorneurons J. Cell Biol., November 30, 1998; 143(5): 1283 - 1294. [Abstract] [Full Text] [PDF]

				S. Zakharenko and S. Popov Dynamics of Axonal Microtubules Regulate the Topology of New Membrane Insertion into the Growing Neurites J. Cell Biol., November 16, 1998; 143(4): 1077 - 1086. [Abstract] [Full Text] [PDF]

				I. Tint, T. Slaughter, I. Fischer, and M. M. Black Acute Inactivation of Tau Has No Effect on Dynamics of Microtubules in Growing Axons of Cultured Sympathetic Neurons J. Neurosci., November 1, 1998; 18(21): 8660 - 8673. [Abstract] [Full Text] [PDF]

				K. E. Sawin and P. Nurse Regulation of Cell Polarity by Microtubules in Fission Yeast J. Cell Biol., July 27, 1998; 142(2): 457 - 471. [Abstract] [Full Text] [PDF]

				T. Kinnunen, M. Kaksonen, J. Saarinen, N. Kalkkinen, H. B. Peng, and H. Rauvala Cortactin-Src Kinase Signaling Pathway Is Involved in N-syndecan-dependent Neurite Outgrowth J. Biol. Chem., April 24, 1998; 273(17): 10702 - 10708. [Abstract] [Full Text] [PDF]

				D. M. Suter, L. D. Errante, V. Belotserkovsky, and P. Forscher The Ig Superfamily Cell Adhesion Molecule, apCAM, Mediates Growth Cone Steering by Substrate-Cytoskeletal Coupling J. Cell Biol., April 6, 1998; 141(1): 227 - 240. [Abstract] [Full Text] [PDF]

				B. Antonsson, D. B. Kassel, G. Di Paolo, R. Lutjens, B. M. Riederer, and G. Grenningloh Identification of in Vitro Phosphorylation Sites in the Growth Cone Protein SCG10. EFFECT OF PHOSPHORYLATION SITE MUTANTS ON MICROTUBULE-DESTABILIZING ACTIVITY J. Biol. Chem., April 3, 1998; 273(14): 8439 - 8446. [Abstract] [Full Text] [PDF]

				A Rajnicek and C McCaig Guidance of CNS growth cones by substratum grooves and ridges: effects of inhibitors of the cytoskeleton, calcium channels and signal transduction pathways J. Cell Sci., January 12, 1997; 110(23): 2915 - 2924. [Abstract] [PDF]

				Y. Gu and Y. Ihara Evidence That Collapsin Response Mediator Protein-2 Is Involved in the Dynamics of Microtubules J. Biol. Chem., June 9, 2000; 275(24): 17917 - 17920. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help