 |
||||
|
||||
|
||||
|
||||
Previous Article | Next Article
Volume 17, Number 9, Issue of May 1, 1997 pp. 3157-3167
Copyright ©1997 Society for NeuroscienceThe 5-HT3 Receptor Is Present in Different Subpopulations of GABAergic Neurons in the Rat Telencephalon
Received Oct. 1, 1996; revised Jan. 27, 1997; accepted Feb. 10, 1997.
Marisela Morales and Floyd E. Bloom The Scripps Research Institute, Department of Neuropharmacology, La Jolla, California 92037
The type 3 serotonin receptor (5-HT3R) is a ligand-gated ion channel whose presence in the CNS has been established by radioligand binding, in situ hybridization, and immunohistochemical analysis. To analyze further the role of the 5-HT3R in the CNS, we used in situ hybridization and immunocytochemistry to determine that 5-HT3R-expressing neurons are mainly GABA-containing cells in the rat telencephalon. We determined that 5-HT3R/GABA-containing neurons do not exhibit somatostatin immunoreactivity but often contain cholecystokinin (CCK) immunoreactivity. 5-HT3R-expressing cells with CCK immunoreactivity were observed in the neocortex, olfactory cortex, hippocampus, and amygdala. The 5-HT3R/CCK interneurons represent between 35 and 66% of the total population of CCK-containing cells in the neocortex.
Further characterization of the 5-HT3R/GABAergic neurons was based on their calcium-binding protein immunoreactivity and showed that these neurons lack parvalbumin (PV) and represent a subpopulation of calbindin (CB)-containing interneurons that were preferentially present in the CA1-CA3 subfield of the hippocampus. Although some 5-HT3R/GABAergic neurons with calretinin (CR) were found in the neocortex, olfactory cortex, hippocampus, and amygdala, these neurons were more often present in the agranular insular and piriform cortices.
We conclude that the neuronal expression of the 5-HT3R is selective within the GABA neuron population in the rat telencephalon. These 5-HT3R-expressing interneurons might contain CCK, CB, and CR. We suggest that serotonin through the 5-HT3R may regulate GABA and CCK neurotransmission in the telencephalon.
Key words: 5-HT3R; serotonin; GABA; interneurons; calcium-binding proteins; cholecystokinin
This article has been cited by other articles:
Y. Noam, W. J. Wadman, and J. A. van Hooft
On the voltage-dependent Ca2+ block of serotonin 5-HT3 receptors: a critical role of intracellular phosphates
J. Physiol., August 1, 2008; 586(15): 3629 - 3638.
[Abstract] [Full Text] [PDF]
J. Jackson, C. T. Dickson, and B. H. Bland
Median Raphe Stimulation Disrupts Hippocampal Theta Via Rapid Inhibition and State-Dependent Phase Reset of Theta-Related Neural Circuitry
J Neurophysiol, June 1, 2008; 99(6): 3009 - 3026.
[Abstract] [Full Text] [PDF]
W. Xiong, M. Hosoi, B.-N. Koo, and L. Zhang
Anandamide Inhibition of 5-HT3A Receptors Varies with Receptor Density and Desensitization
Mol. Pharmacol., February 1, 2008; 73(2): 314 - 322.
[Abstract] [Full Text] [PDF]
K. B. Fink and M. Gothert
5-HT Receptor Regulation of Neurotransmitter Release
Pharmacol. Rev., December 1, 2007; 59(4): 360 - 417.
[Abstract] [Full Text] [PDF]
S. A. Hart, M. A. Snyder, T. Smejkalova, and C. S. Woolley
Estrogen Mobilizes a Subset of Estrogen Receptor-{alpha}-Immunoreactive Vesicles in Inhibitory Presynaptic Boutons in Hippocampal CA1
J. Neurosci., February 21, 2007; 27(8): 2102 - 2111.
[Abstract] [Full Text] [PDF]
B. Kocsis, V. Varga, L. Dahan, and A. Sik
Serotonergic neuron diversity: Identification of raphe neurons with discharges time-locked to the hippocampal theta rhythm
PNAS, January 24, 2006; 103(4): 1059 - 1064.
[Abstract] [Full Text] [PDF]
J. D. Shepard, Y. Liu, P. Sassone-Corsi, and G. Aguilera
Role of Glucocorticoids and cAMP-Mediated Repression in Limiting Corticotropin-Releasing Hormone Transcription during Stress
J. Neurosci., April 20, 2005; 25(16): 4073 - 4081.
[Abstract] [Full Text] [PDF]
M. V. Puig, F. Artigas, and P. Celada
Modulation of the Activity of Pyramidal Neurons in Rat Prefrontal Cortex by Raphe Stimulation In Vivo: Involvement of Serotonin and GABA
Cereb Cortex, January 1, 2005; 15(1): 1 - 14.
[Abstract] [Full Text] [PDF]
P. Somogyi and T. Klausberger
Defined types of cortical interneurone structure space and spike timing in the hippocampus
J. Physiol., January 1, 2005; 562(1): 9 - 26.
[Abstract] [Full Text] [PDF]
M. Blatow, A. Caputi, and H. Monyer
Molecular diversity of neocortical GABAergic interneurones
J. Physiol., January 1, 2005; 562(1): 99 - 105.
[Abstract] [Full Text] [PDF]
M. V. Puig, N. Santana, P. Celada, G. Mengod, and F. Artigas
In Vivo Excitation of GABA Interneurons in the Medial Prefrontal Cortex through 5-HT3 Receptors
Cereb Cortex, December 1, 2004; 14(12): 1365 - 1375.
[Abstract] [Full Text] [PDF]
N. Santana, A. Bortolozzi, J. Serrats, G. Mengod, and F. Artigas
Expression of Serotonin1A and Serotonin2A Receptors in Pyramidal and GABAergic Neurons of the Rat Prefrontal Cortex
Cereb Cortex, October 1, 2004; 14(10): 1100 - 1109.
[Abstract] [Full Text] [PDF]
A. Neumeister, A. C. Nugent, T. Waldeck, M. Geraci, M. Schwarz, O. Bonne, E. E. Bain, D. A. Luckenbaugh, P. Herscovitch, D. S. Charney, et al.
Neural and Behavioral Responses to Tryptophan Depletion in Unmedicated Patients With Remitted Major Depressive Disorder and Controls
Arch Gen Psychiatry, August 1, 2004; 61(8): 765 - 773.
[Abstract] [Full Text] [PDF]
A. V. Harrell and A. M. Allan
Improvements in Hippocampal-Dependent Learning and Decremental Attention in 5-HT3 Receptor Overexpressing Mice
Learn. Mem., September 1, 2003; 10(5): 410 - 419.
[Abstract] [Full Text] [PDF]
T. F. FREUND, I. KATONA, and D. PIOMELLI
Role of Endogenous Cannabinoids in Synaptic Signaling
Physiol Rev, July 1, 2003; 83(3): 1017 - 1066.
[Abstract] [Full Text] [PDF]
J. A. van Hooft and W. J. Wadman
Ca2+ Ions Block and Permeate Serotonin 5-HT3 Receptor Channels in Rat Hippocampal Interneurons
J Neurophysiol, April 1, 2003; 89(4): 1864 - 1869.
[Abstract] [Full Text] [PDF]
Z. Xiang and D. A. Prince
Heterogeneous Actions of Serotonin on Interneurons in Rat Visual Cortex
J Neurophysiol, March 1, 2003; 89(3): 1278 - 1287.
[Abstract] [Full Text] [PDF]
R. C. Foehring, J. F. M. van Brederode, G. A. Kinney, and W. J. Spain
Serotonergic Modulation of Supragranular Neurons in Rat Sensorimotor Cortex
J. Neurosci., September 15, 2002; 22(18): 8238 - 8250.
[Abstract] [Full Text] [PDF]
I. Ferezou, B. Cauli, E. L. Hill, J. Rossier, E. Hamel, and B. Lambolez
5-HT3 Receptors Mediate Serotonergic Fast Synaptic Excitation of Neocortical Vasoactive Intestinal Peptide/Cholecystokinin Interneurons
J. Neurosci., September 1, 2002; 22(17): 7389 - 7397.
[Abstract] [Full Text] [PDF]
M. Morales and S.-D. Wang
Differential Composition of 5-Hydroxytryptamine3 Receptors Synthesized in the Rat CNS and Peripheral Nervous System
J. Neurosci., August 1, 2002; 22(15): 6732 - 6741.
[Abstract] [Full Text] [PDF]
F.-M. Zhou and J. J. Hablitz
Activation of Serotonin Receptors Modulates Synaptic Transmission in Rat Cerebral Cortex
J Neurophysiol, December 1, 1999; 82(6): 2989 - 2999.
[Abstract] [Full Text] [PDF]
C.D. Paspalas and G.C. Papadopoulos
Noradrenergic Innervation of Peptidergic Interneurons in the Rat Visual Cortex
Cereb Cortex, December 1, 1999; 9(8): 844 - 853.
[Abstract] [Full Text] [PDF]
A. E. Dubin, R. Huvar, M. R. D'Andrea, J. Pyati, J. Y. Zhu, K. C. Joy, S. J. Wilson, J. E. Galindo, C. A. Glass, L. Luo, et al.
The Pharmacological and Functional Characteristics of the Serotonin 5-HT3A Receptor Are Specifically Modified by a 5-HT3B Receptor Subunit
J. Biol. Chem., October 22, 1999; 274(43): 30799 - 30810.
[Abstract] [Full Text] [PDF]
D. D. Mott, Q. Li, M. M. Okazaki, D. A. Turner, and D. V. Lewis
GABAB-Receptor-Mediated Currents in Interneurons of the Dentate-Hilus Border
J Neurophysiol, September 1, 1999; 82(3): 1438 - 1450.
[Abstract] [Full Text] [PDF]
D. G. Rainnie
Serotonergic Modulation of Neurotransmission in the Rat Basolateral Amygdala
J Neurophysiol, July 1, 1999; 82(1): 69 - 85.
[Abstract] [Full Text] [PDF]
K. R. Svoboda, C. E. Adams, and C. R. Lupica
Opioid Receptor Subtype Expression Defines Morphologically Distinct Classes of Hippocampal Interneurons
J. Neurosci., January 1, 1999; 19(1): 85 - 95.
[Abstract] [Full Text] [PDF]
G. E. Stutzmann, B. S. McEwen, and J. E. LeDoux
Serotonin Modulation of Sensory Inputs to the Lateral Amygdala: Dependency on Corticosterone
J. Neurosci., November 15, 1998; 18(22): 9529 - 9538.
[Abstract] [Full Text] [PDF]
Y. Kawaguchi and T. Shindou
Noradrenergic Excitation and Inhibition of GABAergic Cell Types in Rat Frontal Cortex
J. Neurosci., September 1, 1998; 18(17): 6963 - 6976.
[Abstract] [Full Text] [PDF]
P. De Deurwaerdere, L. Stinus, and U. Spampinato
Opposite Change of In Vivo Dopamine Release in the Rat Nucleus Accumbens and Striatum That Follows Electrical Stimulation of Dorsal Raphe Nucleus: Role of 5-HT3 Receptors
J. Neurosci., August 15, 1998; 18(16): 6528 - 6538.
[Abstract] [Full Text] [PDF]
B. Roerig and L. C. Katz
Modulation of Intrinsic Circuits by Serotonin 5-HT3 Receptors in Developing Ferret Visual Cortex
J. Neurosci., November 1, 1997; 17(21): 8324 - 8338.
[Abstract] [Full Text] [PDF]
G. E. Stutzmann and J. E. LeDoux
GABAergic Antagonists Block the Inhibitory Effects of Serotonin in the Lateral Amygdala: A Mechanism for Modulation of Sensory Inputs Related to Fear Conditioning
J. Neurosci., June 1, 1999; 19(11): RC8 - RC8.
[Abstract] [Full Text] [PDF]
I. Aradi and I. Soltesz
Modulation of network behaviour by changes in variance in interneuronal properties
J. Physiol., January 1, 2002; 538(1): 227 - 251.
[Abstract] [Full Text] [PDF]
|
|
|
|
 |
|